I. S. Falk and C.-E. A. Winslow 459 
Effects on Pancreatin. 
The influence of bromate upon the action of pancreatin was 
next studied in the same way. The buffer solution in this case 
was made up with 50 cc. of 0.067 m K,HPQu,, 17.5 ec. of 0.2 nN 
NaOH, and 82.5 ce. of water, giving an initial pH value of 10.8. 
The titration method was the same as outlined above. 
Experiment V.—July 30, 1917. 3 gm. of Merck’s casein, 0.5 gm. of 
Merck’s pancreatin, to 100 cc. Digestion period, 53 hours. 
Increase in amino-acids in 25 
Solution. cc. Cc.0.2 Nn NaOH to 
neutralize. 
Buffer + casein. 
(73 6“ wa ' 0.85 
«“ « + pancreatin. 4.00 
“ “ “ ; 4.20 3.95 
: “ ‘c 3.70 
‘“ “e «+ KBrO; 1: 100 3.90 
‘ ‘ 66 Ce 3.45° 3,95 
‘< “ 6c 66 4.45 
‘“ <3 ‘“ Ss 1: 500 3.85 
“« ‘“ “ “ 3.60 3.60 
“ ‘< 73 6c 3407” 
“ “ “ 6c ie 1,000 2.70 
‘“ “ “ a 3.10 3.00 
(73 66 “ (15 3.10 
‘ « “ “ 1; 10,000 3.70 
“ “c 66 OS Oro OU 
“ “ 6 6 3.50 
This experiment appears to indicate that bromate in strong 
concentrations (1: 100 to 1: 10,000) exerts an inhibitive action 
upon pancreatin. | 
