500 Preparation of Taurin 
led us to look for an easier method. In our hands the method 
described below has given better yields of taurin than that used 
by Miyake and has enabled us to work with larger quantities of 
material at less expense. Several kilos of taurin hawe been pre- 
pared by this method. | : 
After removal of the shell and intestinal tract of the abalone, 
the muscle is finely ground, heated in a steam sterilizer for a 
number of hours, and the juice removed by pressure. The re- 
sultant cake is reground and again extracted by addition of dis- 
tilled water and heating. To each 18 liters of the combined ex- 
tracts is added sufficient glacial acetic acid (about 15 cc.) to 
make the concentration 0.02 n. This gives the maximum pre- 
cipitate of protein. After heating for several hours the liquid 
is transferred to settling bottles, the clear supernatant liquid 
syphoned off, and the remainder filtered. To the combined fil- 
trates 100 cc. of strong hydrochloric acid are added, the mixture 
is again heated, and a small additional precipitate removed as 
before. The clear liquid is evaporated to about one-third of its 
volume, 500 ce. of strong hydrochloric acid are added, and the 
mixture boiled until a test sample shows no precipitation of pro- 
tein on addition of several volumes of alcohol. The liquid is 
further concentrated and taurin precipitated by the addition of 
sufficient alcohol to give a concentration of 60 to 70 per cent. 
On cooling, large amounts of taurin crystals separate, which are 
filtered and washed with 60 per cent alcohol. The taurin is fur- 
ther purified by boiling with charcoal, crystallizing from water, 
and washing the crystals with small amounts of water until free 
from sulfates. Determination of sulfur gave theoretical values. 
Eight dozen abalones (74 kilos of muscle) gave a yield of 340 
gm. of taurin. 
An additional yield of 22 gm. was obtained by precipitating 
the sulfates in the mother liquor by addition of barium hydroxide 
and crystallizing the taurin. This gave a total yield of 362 gm. 
