1V Scientific Proceedings 
confidence in the correctness of our conclusions, these observa- 
tions are not to be’ considered as necessarily final. 
When kafir forms the sole diet of white rats, it has about the 
same nutritional value as. corn. Kafir is seriously deficient in 
calcium, but when this element (Ca lactate) is supplied, the ani- 
mals grow and attain maturity, though the growth period is con- 
siderably prolonged. Growth on kafir and ash mixture is ap- 
parently more rapid than on corn and ash mixture. 
Females receiving kafir and a salt mixture became opens 
and delivered young, but in no case did these litters survive more 
than a few days. | 
The proteins of kafir seemed to have a biological value equal 
to that of corn in inducing growth. 
Kafir is probably deficient in one of the accessory substances. 
A comparison of the data obtained by feeding kafir and by feed- 
ing corn indicates that the fat-soluble A, as described by McCollum, 
is a limiting factor in kafir. 
Our investigations.on the nutritive properties of kafirin were 
brought more nearly to completion and the data has been pub- 
lished in detail.!. Kafirin, when it forms the sole source of pro- 
tein in the diet, is apparently inadequate for growth or for the 
maintenance of young animals (rats). Lysine is the first limit- 
ing factor of kafirin, and cystine is the second. The results in- 
dicate that lysine is indispensable not only’ for growth, but also 
for the maintenance of young animals. 
THE RELATION BETWEEN PHOSPHORUS METABOLISM AND THE 
SECRETION OF MILK FAT. 
By EDWARD B. MEIGS anp N. R. BLATHERWICK. 
(From the Bureau, of Animal Industry, United States Department of 
Agriculture, Washington.) 
Blood samples have been obtained nearly simultaneously from 
the jugular and mammary veins of milking cows, and the plasma 
from such samples has been analyzed for total, lipoid, and inor- 
ganic phosphorus, the object being to determine whether the 
concentrations of these kinds of phosphorus were appreciably 
1 Hogan, A. G., J. Biol. Chem., 1918, xxxiii, 151. 
