XV1 Scientific Proceedings 
action caused increases in the uric acid excretion of even greater 
magnitude than glycocollor alanine. Neither urea nor ammonium 
chloride administered in amounts comparable to the amino-acids 
had any influence on the uric acid output. These results are be- 
lieved to indicate that the rise in uric acid excretion following 
ingestion of protein food is not due mainly to the work of the di- 
gestive glands but to stimulation of the cellular metabolism by 
the amino-acids liberated in the digestion of the protein. Cre- 
atinine excretion was not affected in any of the experiments. 
Uric acid was determined colorimetrically according to Benedict 
and Hitchcock. 
ASPECTS OF HIPPURIC ACID CONJUGATION. 
By E. LACKNER, A. LEVINSON, anp WITHROW MORSE. 
(From the Nelson Morris Institute, Chicago.) 
The liver is concerned with several types of pairing, yet Sal- 
kowski reported that the principal seat of hippuric acid conjuga- 
tion is resident in the kidney. Salkowski’s observations have been 
repeated by others, and variations in the sweeping statements 
given by him have had to be made. In the dog, Kingsbury 
found that there are other organs concerned with this type of 
pairing. The purpose of the following experiments was first of 
all to determine whether the liver played a recognizable réle in 
hippuric acid synthesis, and, secondly, whether, if this is true, 
the principle could be utilized in detecting obscure liver involve- 
ments in man. 
Two male dogs were isolated’in metabolism cages and the food, 
water, and excretions observed over a period of 34 days. The 
urine was collected in 24 hour quantities. Hepatic involvement 
was made by the use of hydrazine sulfate, which, according to 
Underhill and Kleiner and to Gideon Wells, produced liver lesions _ 
while leaving the kidneys untouched. The truth of this is vouched 
for by the chloride figures of the present set of experiments as 
well as by the evidence of autopsy. Hippuric acid was deter- 
mined by the Folin-Flanders method; chlorides were determined 
by the method of Volhard; gasometric amino nitrogen determi- 
nations were made upon the hydrolyzed urine to determine the 
glycocoll participation. 
