uséd, Subsequent work has shown no evidence that any fish survived this treatment, 
The poison was applied at 5:50 p.m., Jume 7, 1938, Within 15 minutes six fish were 
seen struggling at the surface of the water. As many more were seen within the next 
10 minutes, in addition to several hundred inch—long buffalo fry that were dying, 
Within an hour redmouth buffalo and carp wére gasping at the surface and the smaller 
kinds of fishes were dying rapidly, Before dark, three men had gathered up 748 black 
bullhneads, 16 large buffalo and 3 carp. 
When fish have been poisoned some of them float and can be picked up immediately, 
but a good share of them sink, In turbid water, or in deep water, dead fish cannot be 
gathered up until they bloat and rise to the surface, The surface of the lake was 
entirely cleared of fish each day for four days after the dose of poison. ‘The numbers 
of each of the important species handled each day are shown in table 2, All of the 
fish handled after the first day had risen from the bottom of the lake, 
TABLE 2,—-NUMBERS OF FISHES RISING TO THE 
SURFACE EACH DAY AFTER POISONING 
First Second Third Fourth 
Day Day Day Day 
Sune 5 N 
6 
Largemouth bass... 
White crappie .... 
Warmouth bass .... 
River ly ret oie ee 
Green sunfish .... 
Orauge-spotted sunfish, 
Channel<catie sn. 6: 6 
Black bullhead. ... 
Redmouth buffalo, , 
Carp. ceeee#s#e © 
Golden shiner... 
e e . e e 
e ° e e e e ° ° J ° e 
e e e ® ® ° e e e e e 
e e e e e e e e °s e . 
s e e e se e 
Twenty-two hours after the poison was applied collections were made with dip nets 
and minnow seiries to find out whether other animal life had been affected, Samples were 
taken from the northwest shore of the pond (where the concentration of poison was 
highest) and from mud dipped from deep water, The numbers and kinds of living and dead 
invertebrates are given in table 3, The poison in the ¢oncentration used seems to be 
TABLE 3,--KINDS AND NUMBERS OF AQUATIC INVERTEBRATES 
Crayfishes. .. 
Mayfly mymphs , 
Hydroporus. .. 
Palpomyia .. 
Midge larvae. , 
Pulmonate snails, 
Dragonfly nymphs, 
Oligochaetes, .. 
° ® + * e e e e 
a e e e s e e* @ 
Specific for fish, Most of the invertebrates were unaffected, and even gill—breathing 
vertebrates other than fish, such as frog tadpoles, survived, 
