Dalhousie Springs hydrobiids y 
material used in the genetic analysis is listed in Appendix 1 (see also Ponder, 1989 for overall 
locality details). Latitudes and longitudes have been calculated using a GIS system (Maplnfo ver. 
3.01; Mapping Information Systems, 1985-1991), after digitizing the spring details based on the 
locality map in Zeidler and Ponder (1989, fig. 2). The spring numbers used follow Zeidler and 
Ponder (1989) and are shown in Fig. 1. 
Morphology studies. Dissection and preparation of material for SEM were by standard methods 
(Ponder er а!., 1993). Morphological observations presented here for the three new species are based 
Оп the type populations. The majority of populations have also been examined in less detail to 
confirm the main anatomical characters and a summary of their shell measurements and ratios are 
presented (Appendix 2). 
Shell measurements were obtained using the methods described by Ponder ef al. (1989), those 
Parameters measured being listed below. The number of opercular pegs was counted only in the 
Species of Fluvidona, those in the new genus being too ill-formed in many specimens to count 
accurately. In the statistics given in each description male and female shell measurements have been 
Pooled and the range of morphology taken from the total pool of data available for that locality. 
hese are given separately in the measurement tables presented for each type lot and in Appendix 2 
(Tables 2 and 3). Statistical analysis was, performed using SYSTAT (Wilkinson, 1992). Material is 
held in the South Australian Museum, Adelaide (SAM) and the Australian Museum, Sydney (AMS). 
Abbreviations used in tables of measurements: 
AL — shell aperture length; AW — shell aperture width; BW — length of last (body) whorl of shell; 
Cv - convexity ratio of penultimate whorl of shell; OL — length of operculum; OS - length of white 
орегсшаг smear; OW — width of operculum; PH - height of longest opercular peg; PL — length of 
area occupied by opercular pegs; PN — number of opercular pegs; SL — shell length; SW — shell 
Width; TW — number of teleoconch whorls. 
Electrophoresis. Standard methods for cellulose acetate electrophoresis were used (Richardson et al., 
1986; Hebert and Beaton, 1989; Ponder et al., 1991). Snails were homogenized in about 20 pl (10 ul 
for small individuals) of buffer, providing enough sample for up to 12 gels. Where more than one 
locus encoding the same enzyme was found, they were designated numerically in order of decreasing 
mobility, Allozymes identified for each locus within a species are designated in the same way. The 
enzymes scored for each species, together with abbreviations, Enzyme Commission Numbers, and 
the number of loci are listed in Table 1. The computer package BIOSYS-1 (Swofford and Selander, 
1989) was used to assist analysis. 
Table 1. The numbers of interpretable loci. The columns give the enzyme name, its abbreviation, E.C. number and the 
number of interpretable loci for: A, Dalhousia spp.; and B, Fluvidona. 
ENZYME ABBREVIATION E.C. NO. A B 
Alcohol dehydrogenase ADH 1.1.1.1 1 
Ikaline phosphatase ALEP 3.1.3.1 1 1 
Spartate aminotransferase AAT 2.6.1.1 2 2 
Enolase ENO 42.1.11 1 1 
Esterase EST ЫН! 3 B 
Fumarate hydratase FH 4.2.1.2 1 
-Galactosidase GAL 3.2.1.23 1 
Glycerol-3-phosphate dehyd. GPD 1.1.1.8 І 1 
lucosephosphate isomerase GPI 5.3.1.9 1 1 
lutamate-pyruvate transamin. GPT 2.6.1.2 1 
Hexokinase HK 2.7.1.1 1 
Hydroxybutyrate dehydrogenase HBDH 1.1.1.30 1 
Isocitrate dehydrogenase IDH 1.1.1.42 2 2 
Leucine aminopeptidase LAP 34.11 1 
Malate dehydrogenase MDH 1.1.1.37 2 2 
