346 © QUEENSLAND AGRICULTURAL JOURNAL. [1 Sepr., 1901. 
As I have said further back, the experiments of Nicolle and Adil-Bey 
furnish a fresh proof of the persistence of the Pzroplasma in the organism; 
they show, moreover, that the action of the microbe of bovine plague is parti- 
cularly favourable to the Piroplasma. 
There is no cattle plague in the Argentine ; nevertheless, I have tried to 
find out if there might not be other diseases to take its place. I have tried 
the bacteria of anthrax, some Pasteurella,* and hog-cholera. 
In the trial by anthrax I have never succeeded in inducing the re-appear- 
ance of the Pzroplasma in the organisms of animals which had already suffered 
from malaria, however many had succumbed to the effects of injection of 
anthrax. With the Pasteurella, and notably with the cocco-bacillus of boyine 
pasteurellosis, I have succeeded, but it was with the greatest difficulty, and in 
the long run, only by making the animals completely cachectic. 
Baeample: Karly in April, 1899, the malaria was raging ina herd of cattle in 
the province of Santa Fé. I.asked for several convalescent beasts, and these arrived 
at my laboratory very much emaciated on the 25th of the same month. I had them 
isolated in stalls, as they were harbourers of ticks. 
On July 3 these animals were in better condition; one of them received a sub- 
cutaneous injection of 3 c.c. of a slightly virulent culture of bovine pasteurellose ; 
temperature, 38°5 C. I took the temperature every day, and drew a few drops of 
blood from the jugular for the purpose of microscopic examination. The inoculations 
with cultures of bovine pasteurellose were as under :— 
July 6.+£90 ¢.c. of culture (in peptone broth), sub-cutaneous. 
10.—70 «.c. iF 3 ip 
12.—4 ¢.c. 2 (peritoneum of guinea-pig), sub-cutaneous. 
» 13.—10 ec. 5 ( 4 i; ) 5 
» 16.—20 c.c. A ( ey a: ) mp 
» 20.—15 c.c. _ i ¥} af 
The animal had abscesses at the points of injection; it became emaciated, was 
anemic, and had the fever after each tresh inoculation. 
July 24.—20 c.c. of culture in broth in the jugular. 
July 25.—Temperature, 41°8 C. 
July 26.—Temperature, 41°5 C. 
July 27.—Temperature, 41°3 C. 
July 28.—Temperature, 41° C. 
July 29.—Temperature, 40°8 C. U 
July 30.—Temperature, 38°8 C. The animal was very ill, anemic and emaciated, 
but at no time from the beginning of the experiment were any hematozoa present in 
the corpuscles. 
From Ist to 9th August, it became daily weaker; still, it fed a little. 
On 10th August I found, in a preparation, four or five red corpuscles with 
heematozoic types. ‘The temperature was 39° C. 
On the 11th I found four. infected corpuscles. The animal was now unable to 
rise; it had an arthritis in each hock, determined by injections of pasteurellose; 
temperature, 39°2 C. 
On the 12th I saw two corpuscles containing Piroplasma; temperature, 39°7 C. 
August 131h.—Temperature, 39° C. Again an infected corpuscle. 
August 14th.—Temperature, 39°1. Nothing to be seen in the corpuscles; the 
animal was very sick, ate almost nothing, and was reduced to the condition of a skeleton. 
August 15th.—Temperature, 37°9 C. Nothing in the corpuscles. 
August 16th.—Temperature, 37°5 OC. Nothing in the corpuscles. The animal 
appeared to be at the point of death. 
August 17th.—Temperature, 35°5 C. Itdiedin the evening. Atthe post-mortem, 
apart from the anemia, I found no lesion whatever which denoted malaria, and 
nowhere did I find the Piroplasma. 
The appearance of hematozoa was certainly favoured by the injections, for 
ordinarily, when they are observed to re-appear spontaneously, it is only to 
disappear on the following day ; in the example given here, on the contrary, 
there was a true mild attack. 
I have also made experiments with hog-cholera, but succeeded no better—a 
fact which proves the specially fayourable action of the micro-organism of 
bovine plague for the Piroplasma bigeminum. 
* Of the whole group of hemorrhagic septicemia. 
. 
es ee 
rE nt 
_— 
a 
