1 Sepr., 1901.] QUEENSLAND AGRICULTURAL JOURNAL. 347 
From the preceding remarks we may conclude that it is not sufficient to 
find, after re-inoculation, an infected corpuscle in the blood of an animal which 
has recovered from malaria, to incriminate this second inoculation, since without 
the latter, some infected corpuscles might have been seen. When there is a 
true re-infection, Piroplasma are found in the corpuscles for several days in 
succession. 
As T have stated, this re-infection should always exist in adult cases, 
according to Nicolle and Adil-Bey. Now, my experiments prove the absolute 
contrary. 
The intra-venous or sub-cutaneous injection of a strong dose of malarial 
blood has absolutely no effect on animals which have lately resisted a first 
attack, especially it it has been a severe one. 
Here is one example from amongst many others :— 
On the 14th July, a cross-bred Durham bullock received a sub-cutaneous 
injection of 10 c.c. of virulent blood. For a few days thereafter it took the disease in 
a virulent form, with hemoglobinurea for three days. 
On the 23rd this animal was convalescent. On the 31st it was fairly well, had a 
good appetite, but was very anemic and emaciated ; since the 23rd, no more hzematozoa 
were found in the blood corpuscles. 1 injected into the jugular 10 c.c. of blood very 
rich in virulent hematozoa, which I found in a control. The blood was examined 
every day. On 9th August, { found a hematozoa; on the 14th I saw two corpuscles 
infected ; on the 18th, one. ‘During the interval, the animal disclosed no hematozoa 
in the corpuscles until Ist September, when I ceased to examine the blood daily. 
The second inoculation did not at all interfere with the convalescence of the 
animal, and I found no more hematozoa in its blood than in that of those which had 
not been inoculated a second time. 
Better still, if the second inoculation is effected at the precise moment 
when the hematozoa begin to disappear, it will produce no effect. I detail in 
this place a convincing experiment :— 
On July 31st, 1899, a bullock was inoculated sub-cutaneously with 10 c.c. of 
virulent blood. Temperature, 38°4 C. 
August 1—Temp., 38°7 C. 
Fe 2— ,, 39°90. There were a few hematozoa in the corpuscles. 
August 3— ,, 41°C. Many hematozoa; in the evening the urine was 
faintly tinged with red. 
August 4.—Temp., 41°4.C. Many Piroplasma; urine very red. There were 
only 2,000,000 red corpuscles per m.m.c. ; 
August 5.—Temp., 40°7 C. Urine, very red; animal lying down, very ill; 
hematozoa less numerous. 
August 6.—Temp., 3799 C. Animal improved; urine no longer red; began to 
feed ; hamatozoa scarce. 
In the evening, the subject received a sub-cutaneous injection of 20 c.c. of virulent 
blood, containing a large number of hematozoa. 
__ August 7.—Temp., 37°8 C. Continued improvement; the appetite returned a 
little; the urine, which was of normal colour, contained albumen. The hematozoa 
were exceedingly rare in the corpuscles. 
August 8.—Temp., 37°5 C. No hematozoa. 
» 9—  , 38°96 C. No hematozoa in the blood, but some giant cells, 
many of which are multi-nucleated. 
Aygust 10.—Temp., 38°3 C. No hematozoa. 
»  ll— ,, 38°70. Nothing in the corpuscles. 
» 1— , 38°99C. NoPiroplasma,butmany giantmulti-nucleated cells. 
August 138.—Temp., 38°8 C. Only in one corpuscle did I see a hematozoic type. 
» 14— ,, 38°9C. Nothing in the corpuscles. 
»  16—  ,, 38°8C. One corpuscle contained a hematozoa. 
» 16— ,, 38°96 C. No hematozoa; multi-nucleated cells rare. 
» L7— ,, 38°9C. Nothing in the corpuscles. 
» I8— , 38°92 C. No Piroplasma. 
7, abs Ts behetes any othing in the corpuscles. 
»  20.— ,, 38°3C. Micro-organisms in the corpuscles still absent. 
» 21— 4, 38°60. One corpuscle contained two types of hematozoa. 
» 23— ,, 39° ©. Nothing in the corpuscles. 
_ Up to the 6th September the temperature was normal, and I met with no 
Piroplasma. ‘The corpuscular reparation became normal. 
