1 Ocr., 1901.] QUEENSLAND AGRICULTURAL JOURNAL, ; 4.23 
Analysis of the Crude Ash. 
Per ent. 
Soluble in water ax; os fe a8 .. 73:05 
Insoluble in water, soluble in HCl ... tet ax JISEPA® 
Unburnt carbon $8 a td ae a 7:25 
Phosphorie acid = ae a. og «| APIS 
Chlorine ey, ay, aot ena ite 284 2°48 
Potash eu a ace 4 Jeet ae SA 
Soda se as byl 8 a eH 3°44 
Lime as i as oe in Ne 6°16 
Magnesia xx} Sua xm oe +0 bey 6°88 
Unburnt carbon me a is ibon a 7°25 
Carbonic acid by differ. mee oe ee xx PH 
The result of the analysis was obtained as follows, most operations being 
carried out in duplicate :— 
Per cent. Per cent. 
Extract by petroleum spirit (boiling under 45 degrees C.)... 1°02 1:10 
Extract by ether (water free) chlorophyll ... Sep .. 0-40 0°39 
Extract by absolute aleohol, principally glucose, with a trace 
of tannin, no alkaloids ... 420 cies is bon OB} lost. 
Extract by cold water el, scr ... 27°04 
As the previous treatment with ether and absolute aleohol made some of 
the albuminoids insoluble, a fresh watery extract of the flour was prepared. 
Per cent. Per cent, 
Extract by cold water of air-dried flour 40 «. 28:48 27°96 
This watery extract contained :— 
Ash otic on wh one ots 1°65 1:60 
Total nitrogen... Be: dex oe 344 w= L054 = 1:055 
Soluble albuminoids calculated from nitrogen bon | (EL) 6:59 
Soluble albuminoids coagulated when boiling th 
watery extract, vegetable casein ore oon ao PIES} 0 
Precipitated by absolute alcohol :— 
Ist. Mucilaginous substances ait 00% Ho BPP) 3:14. 
2nd. Dextrin  ... cx 0 oop wc 4:98 6°70 
When treating the watery extract with 4 vols. of absolute alcohol, there is 
adanger that part of the saponin is also precipitated with the dextrin, if the 
filtering is not done very quickly. As the second sample filtered very slowly, 
the dextrin contained a large amount of saponin, by giving the characteristic 
test, when treated with sulphuric acid. 
Not precipitated by absolute alcohol glucose ... auc we §=—-: 064 
For comparison with the usual method of extraction, extracts with benzol 
and 80 per cent. boiling alcohol were also made :— 
Per cent, 
Extract by benzol (C,H,) boiling at 81°C. or a0 ws 48 
Extract by 80 per cent. alcohol (Sp. Gr. 8483 at 15°) ... w. 19°88 
Of this extract was :— 
Soluble in absolute alcohol... vs ae fa 44 ».. 12°50. 
Insoluble in absolute alcohol, but soluble in water, and pre- 
cipitated by subacetate of lead PAE 5 Mi en YP 
The saponin was determined in accordance with the method recommended 
by Christophson and Otten (Dragendorff’s Plant Analysis, p. 68), by extracting 
with boiling 80 per cent. alcohol, filtering when hot, boiling off the aleohol of 
this extract, and precipitating the saponin with concentrated baryta water. 
For the quantitative determination, the residue of the flour, after having been 
treated and extracted with petroleum ether, ether and absolute alcohol were 
used, and gave in an average of several determinations—14°58 per cent. saponin. 
Larger quantities of the glucoside were prepared from fresh samples of the 
