ZooKeys 857: 105-1 38 (20 | 9) A peer-reviewed open-access journal RE RCT #ZooKeys http:/ /Zz00 keys -pen soft.net Launched to accelerate biodiversity research A review of some new or little-known species of the genus Gnorimoschema (Lepidoptera, Gelechiidae) from the Palaearctic region Oleksiy Bidzilya', Peter Huemer’, Kari Nupponen?, Jan Sumpich* | Institute for Evolutionary Ecology of the National Academy of Sciences of Ukraine, 37 Academician Lebedev str, 03143, Kiev, Ukraine 2 Tiroler Landesmuseen Betriebsges.m.b.H., Natural History Collections, Krajnc-Str. 1, A-6060 Hall in Tirol, Austria 3 Merenneidontie 19 D, FI-02320 Espoo, Finland 4 National Museum, Natural History Museum, Department of Entomology, Cirkusovd 1470, CZ-193 00 Praha 9 - Hornt Pocernice, Czech Republic Corresponding author: Oleksiy Bidzilya (olexbid@gmail.com) Academic editor: E. van Nieukerken | Received 28 February 2019 | Accepted 23 April 2019 | Published 25 June 2019 http://zoobank.org/E71 9F FD4-3703-4F78-864D-884997 162527 Citation: Bidzilya O, Huemer P, Nupponen K, Sumpich J (2019) A review of some new or little-known species of the genus Gnorimoschema (Lepidoptera, Gelechiidae) from the Palaearctic region. ZooKeys 857: 105-138. https://doi. org/10.3897/zookeys.857.34188 Abstract Six new species of Gnorimoschema Busck, 1900 are described: G. pamira sp. nov. (Tadzhikistan), G. brachyptera sp. nov. (Russia: Buryatia), G. a/taica sp. nov. (Russia: Altai), G. tabazhok sp. nov. (Russia, Altai, Tuva), G. yakovlevi sp. nov. (Russia: Altai, Buryatia), G. kozlovi sp. nov. (Mongolia). A new syno- nym is established: G. mikkolai Povolny, 1994 syn. nov. of G. radkevichi Piskunov, 1980. Gnorimoschema montanum Povolny, 1966, sp. rev., stat. nov. is taken out from synonymy with G. soffneri (Riedl, 1965). An annotated check-list of the genus Gnorimoschema in the Palaearctic region is provided. Keywords New species, new records, new synonym, systematic, distribution, brachyptery, Russia, Siberia, Tadzhikistan, Mongolia, DNA barcoding Copyright Oleksiy Bidzilya et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 106 Oleksiy Bidzilya et al. / ZooKeys 857: 105-138 (2019) Introduction The Gnorimoschemini is an extremely species rich tribe in the subfamily Gelechiinae. Altogether about 900 species and 44 genera are known world-wide. The tribe is most diverse in the Palaearctic region, where more than 300 species from 21 genera are known (Povolny 2002; Bidzilya and Li 2010; Huemer and Karsholt 2010). The high- est generic diversity is found in the Neotropics, with about 180 species known from the Nearctic region (Povolny 2002; Lee et al. 2009; Huemer and Karsholt 2010). Stud- ies on the Oriental, Australian and Afrotropical fauna of Gnorimoschemini are rather fragmentary; however, the tribe is likely less diverse in these regions than in the Hol- arctic and Nearctic. Despite the progress in the study of Palaearctic Gnorimoschemini in the last decades (Li and Bidzilya 2008; Huemer and Karsholt 2010; Bidzilya and Li 2016), the tribe is still in need of considerable taxonomic and faunistic study especially in central and eastern regions, where the discovery of many new taxa is expected. The classification of the Gelechiidae is under dispute (Ponomarenko 2005; Karsholt et al. 2013). However, authors generally agree to place Gnorimoschemini in the subfamily Gelechiinae. Within this subfamily the Gnorimoschemini share with Gelechiini synapomorphy such as the conspicuous dilation of the lateral parts of the vinculum (Huemer and Karsholt 2010). Povolny (1964, 2002) did not specify auta- pomorphies for Gnorimoschemini, but defined the tribe mainly by a combination of genitalia characters. Currently, the monophyly of the tribe is supported by the hook- like signum and a lateral zone of microtrichia in the ostial area (Huemer and Karsholt 1999, 2010; Ponomarenko 2005). The generic classification of Gnorimoschemini is poorly developed. A phylogeny of the tribe proposed by Povolny and Sustek (1988) and based on methods of numeri- cal taxonomy includes seven groups of genera. These groups are rather weakly defined and their taxonomic status remains uncertain. The diagnostic characters of male and female genitalia of Gnorimoschema Busck, 1900 were recently discussed (Huemer and Karsholt 2010; Li and Bidzilya 2017). Within the tribe, Gnorimoschema is most close- ly related to the Nearctic genus Neoschema Povolny, 1967 and more distantly to the mainly Neotropical genus Symmetrischema Povolny, 1967 (Povolny 1991). Most of the Palaearctic species of Gnorimoschema are difficult to separate from other Gnorimoschemini externally and often are confused with other species, e.g. of the genus Scrobipalpa Janse, 1951. However, some mainly large-sized species of Gnorimoschema are distinguished from other genera by the narrow elongated wings. Additionally, the males can be recognized by the characteristic shape of the uncus and terminal portion of valvae which are usually protruded and clearly visible under a binocular microscope. The species of Gnorimoschema inhabit primarily open landscapes. In the Palaearctic region they are most diverse in xeromontane habitats. Several species are restricted to sand dunes and sandy riverbanks in the northern and central Palaearctic. Gnorimoschema is most diverse in the Nearctic region where 95 species are known (Lee et al. 2009). The Palaearctic species of the genus were studied intensively by Pov- olny, who contributed tremendously to the taxonomy and diversity of the genus (Po- volny 1966, 1967, 1984, 1992, 1994, 2002). After that, Gnorimoschema was later A review of some new or little-known species of the genus Gnorimoschema ... 107 revised in Europe (Huemer and Karsholt 2010) and China (Li and Bidzilya 2017). As a result, twenty-one species have been recorded from the Palaearctic region, the taxonomic state of several species was clarified and some new synonymies proposed. This contribution aims to describe six additional new species from Tadzhikistan, Rus- sia (Southern Siberia) and Mongolia, to clarify the taxonomic state of some species and to add several new country records. The description of new species is supported morphologically and, for the majority, confirmed by DNA barcodes (mtCOI gene). We also provide an annotated check-list which includes all recent changes in taxonomy and distribution of Palaearctic species of Gnorimoschema. Material and methods Specimens Adults were collected by light trapping or by hand netting. Male and female genitalia were dissected and prepared using standard methods (Huemer and Karsholt 1999). The present contribution is based on material deposited in the following collections: LMK Landesmuseum Karnten, Klagenfurt, Austria MZH Finnish Museum of Natural History, Helsinki, Finland NMPC National Museum Prague, Czech Republic NUPP Research collection of Kari & Timo Nupponen, Espoo, Finland SMNK _ Staatliches Museum ftir Naturkunde Karlsruhe, Germany TLMF _ Tiroler Landesmuseum Ferdinandeum, Hall in Tirol, Austria ZIN Zoological Institute Russian Academy of Sciences, Sankt-Petersburg, Russia ZMKU = _ Zoological Museum Kiev Taras Shevchenko National University, Ukraine Photographic documentation Pinned specimens were photographed with an Olympus E-410 digital camera at- tached to an Olympus SZX12 microscope or with Canon 750D and MP-E-65 mm lens. Slide-mounted genitalia were photographed with a Canon EOS 600D digital camera mounted on an Olympus U-CTR30-2 trinocular head combined with a Carl Zeiss microscope body. Sets of 10-20 images were taken for each specimen and assembled to deep-focused images using Helicon Focus 6 and edited in Adobe Photoshop CS5. DNA Barcoding DNA barcode sequences of the mitochondrial COI gene — a 658 base-pair long segment of the 5’ terminus of the mitochondrial COI gene (cytochrome c oxidase 1) 108 Oleksiy Bidzilya et al. / ZooKeys 857: 105-138 (2019) — were obtained from 139 new specimens. Some specimens already sequenced, from private or published data (Mutanen et al. 2016), were also included in our dataset. DNA samples from dried legs were prepared according to prescribed standards using the high-throughput protocol of de Waard et al. (2008). Samples were processed in the Canadian Centre for DNA Barcoding (CCDB, Biodiversity Institute of Ontario, University of Guelph). Sequences were submitted to GenBank. Details of successfully sequenced voucher specimens, including complete geographic data and images, can be accessed in the Barcode of Life Data Systems (BOLD; Ratnasingham and Hebert 2007) in the public dataset “DS-LEPALGNO Lepidoptera of the Palearctic - Gelechiidae/ Gnorimoschema’ dx.doi.org/10.5883/DS-LEPALGNO. Degrees of intra- and interspecific variation in the DNA barcode fragments were calculated under the Kimura 2 parameter (K2P) model of nucleotide substitu- tion using analytical tools in BOLD systems v. 4.0 (http://www.boldsystems.org). A neighbour-joining tree of DNA barcode data of currently sequenced Palaearctic taxa was constructed using MEGAG (Tamura et al. 2013) under the K2P model for nucleotide substitutions. Furthermore, we checked the congruence of taxonomy with Barcode Index Numbers (BIN) proposed by Ratnasingham and Hebert (2013). This system clus- ters sequences into so-called Operational Taxonomic Units (OTUs), regardless of their previous taxonomic assignment. It is based on a two-stage algorithm that groups the sequences in a cluster and automatically assigns new sequences. All sequences > 500 bp and covering some other quality requirements are recorded independently of the project origin and assigned to a BIN (Ratnasingham and Hebert 2013). Ultimately, the BIN system is a tried and tested means of checking the concordance between morpho-taxonomically based species determinations and COI sequence data. Terminology The descriptive terminology of the genitalia structures generally follows Huemer and Karsholt (2010). Results Molecular results From 139 specimens of Gnorimoschema we obtained 113 sequences with 104 barcode sequences longer than 500 bp, which were used for analyses. The sequenced 23 morpho- species all group in different clusters (Fig. 1) and also can be separated at species level. Intraspecific distances range from 0% to 2.98% (mean 0.46%). Distances to the nearest A review of some new or little-known species of the genus Gnorimoschema ... 109 G. epithymella (N=12; AUT, FIN, ITA, NOR) BOLD:AAE7091 G. jalavai (n=3; RUS) BOLD:ADR7753 G. robustella (n=2; RUS) BOLD:ACB0967 G. pamira (n=2; TJK) BOLD:ADF4416 G. vastifica (n=1; RUS) BOLD:AAH4533 G. soffneri (n=1; GRC) BOLD:ADM1971 G. montanum (n=3; AFG) BOLD:ADF2656 G. hrbichii (n=6; DNK) BOLD:AAL5587, BOLD:AAA6744 G. nupponeni (n=4; RUS) BOLD:ACB3130 G. steueri (n=8; ITA, RUS) BOLD: ADE0307 G. radkevichi (n=3; RUS) BOLD:ACA7784 G. nordlandicolella (n=5; FIN, RUS) BOLD:AAA7774 G. streliciella (n=6; FIN, NOR) BOLD:AAF8271 G. fuscescens (n=3; RUS) BOLD:ADE0186 G. nilsi (n=1; AUT) BOLD: AAL3826 G. altaica (n=4; RUS) BOLD:AAI5506 G. brachyptera (n=2; RUS) BOLD:ADF2846 G. yakovlevi (n=2; RUS) BOLD:ADE8232 G. tabazhok (n=10; RUS) BOLD:ADD9963 G. hoefneri (N=6; AUT, ITA, SLO) BOLD:AAF8265 G. rufomaculata (n=1; RUS) BOLD:ADF3327 G. cinctipunctella (n=8; RUS) BOLD:ADE0188 G. valesiella (n=11; ESP, FIN, ITA, NOR, RUS) BOLD:AAA7775 /—— 0.01 = 1% Figure |. Neighbour-joining tree of Palaearctic Gnorimoschema species (Kimura 2 parameter). Note: the scale bar only applies to internal branches between species. The width of the triangles represents the sample size, and the height the relative genetic variation within the cluster (2x scale bar). neighbour vary from min. 1.39% to 6.44% (mean 3.96%) (Table 1). All successfully sequenced species, except for a single species pair (G. brachyptera — G. yakovlevi), are separated by their BINs (Barcode Index Number) in BOLD (Ratnasingham and Hebert 2013). Further information on the genetic results can be found under each species. 110 Oleksiy Bidzilya et al. / ZooKeys 857: 105-138 (2019) Table | COI sequences of Gnorimoschema species in the Palaearctic. Intraspecific mean K2P (Kimura 2 parameter) divergences, maximum pairwise distances, and distance to the nearest neighbour in percentage. Species Mean Intra-Sp Max Intra-Sp Nearest Species Distance to NN G. altaica 0.63 132 G. valesiella 329 G. brachyptera 0 0 G. yakovlevi 1.39 G. cinctipunctella 0.51 G92 G. rufomaculata 293 G. epithymella 0.3 0.8 G. jalavai 3.96 G. fuscescens 0.32 0.49 G. rufomaculata 3.6 G. herbichii 1.57 2.98 G. robustella 3.78 G. hoefneri 0.59 hay G. rufomaculata 3.44 G. jalavai 0.93 139 G. epithymella 3.96 G. montanum 0 0 G. herbichii 4.27 G. nilsi N/A 0 G. altaica 4.93 G. nordlandicolella 0.69 1.6 G. valesiella 4.14 G. nupponeni 0.15 0.31 G. valesiella 5.6 G. pamira 0 0 G. vastifica 5.26 G. radkevichi 0.1 0.15 G. valesiella 5.57 G. robustella 0.15 0.15 G. herbichii 3.78 G. rufomaculata N/A 0 G. cinctipunctella 2.93 G. soffneri N/A 0 G. herbichii 4.52 G. yakovlevi Oal'5 0.15 G. brachyptera 1939 G. steueri 0.89 2.18 G. nupponeni 6.44 G. streliciella 0.05 0.16 G. cinctipunctella 4.11 G. tabazhok 0.16 0.39 G. altaica 4.11 G. valesiella 0.47 0.98 G. altaica 3.29 G. vastifica N/A 0 G. robustella 4.91 Descriptions of new species Gnorimoschema pamira sp. nov. Figs 2, 3, 20, 34 Material examined. Holotype. TADZHIKISTANG; W-Pamir Mts., Pianj/Pamir River by Zugvand village; 37°00'55"N, 72°34'32"E; 2810 m; 25 Jul. 2013; K. Nup- ponen & R. Haverinen leg.; gen. slide 402/16, O. Bidzilya; TLMF Lep 21646; NUPP. Paratypes. 2 9; same data as for holotype; gen. slide 401/16, O. Bidzilya; TLMF Lep 21647; NUPP. Description. Adult (Figs 2, 3). Wingspan 15.8-16.0 mm. Head covered with white, brown-tipped scales; segment II of labial palpus white mixed with brown, inner surface white, with brush of modified scales on underside, segment II brown with white base and apex, acute, scape brown, densely mixed with white, flagellum grey, black-ringed; thorax white mottled with brown, tegulae with several brown scales; forewing covered with white, black-tipped scales, oblique narrow white fascia from about 1/8 of costal margin to 1/3 of fold, sub-costal vein mottled with brown to 2/3 length, dorsal margin brown under distal half of fold, brown spot in fold, short black streak edged with brown in mid wing, longer black streak with brown scales beneath A review of some new or little-known species of the genus Gnorimoschema ... 111 Figures 2-19. Guorimoschema adults 2 G. pamira sp. nov. — HT, male, Pamir (gen. slide 402/16, O. Bidzilya) 3 G. pamira sp. nov. — PT, female, Pamir (gen. slide 401/16, O. Bidzilya) 4 G. brachyptera sp. nov. — HT, female, Buryatia (gen. slide 160/16, O. Bidzilya) 5 G. brachyptera sp. nov. — PT, female, Buryatia 6 G. brachyptera sp. nov. — PT, male, Buryatia (gen. slide 159/16, O. Bidzilya) 7 G. brachyptera sp. nov. — PT, male, Buryatia (gen. slide 240/16, O. Bidzilya) 8 G. altaica sp. nov. — HT, male, Altai (gen. slide 31/18, O. Bidzilya) 9 G. altaica sp. nov. — PT, female, Altai (gen. slide 30/18, O. Bidzilya) 10 G. tabazhok sp. nov. — HT, male, Altai I | G. tabazhok sp. nov. — PT, male, Altai (gen. slide 1250, P. Huemer) 12 G. tabazhok sp. n. — PT, female, Altai (gen. slide 18595, J. Sumpich) 13 G. tabazhok sp. nov. — PT, female, Altai (gen. slide gen. slide GP 3_2.1.2019KN) 14 G. yakovlevi sp. nov. — HT, male, Altai (gen. slide 406/16, O. Bidzilya) 15 G. yakovlevi sp. nov. — PT, male, Altai 16 G. yakovlevi sp. nov. — PT, female, Buryatia (gen. slide 69/18, O. Bidzilya) 17 G. kozlovi sp. nov. — HT, male, Mongolia (gen. slide 236/15, O. Bidzilya) 18 G. radkevichi Pisk. — HT, female, Mongolia 19 G. radkevichi Pisk., male, Altai. 112 Oleksiy Bidzilya et al. / ZooKeys 857: 105-138 (2019) on 2/3 length in cell, diffuse white sub-apical fascia at 34 length, costal margin mottled white before apex, fringe white, black-tipped; hindwing and fringe light grey. Variation. The black pattern of one female paratype is more extensive making the specimen look darker, white basal fascia indistinct (Fig. 3). Male genitalia (Fig. 20). Uncus sub-rectangular, apex triangular, pointed; gnathos weakly curved, gradually narrowed apically; tegumen narrow, anteromedial emargina- tion triangular, extending to about half length of tegumen; valva broad at basal half, curved and constricted in middle, apex narrow, rounded; sacculus straight, as broad as valva in its narrowest mid length, distal portion narrow, strongly curved inwards, gap to vincular process very narrow; vinculum broad, posterior margin with deep and broad sub-ovate medial emargination, lateral process short, sub-rectangular, postero- lateral corner join with the tip of sacculus; saccus broad on base, sub-triangular, apex rounded, not extended beyond top of pedunculus; phallus broad, s-curved, with point- ed apex, caecum inflated, slightly exceeding half length of phallus. Female genitalia (Fig. 34). Papilla analis elongate, sub-ovate, densely covered with short setae; apophysis posterioris twice longer than segment VIII; segment VII evenly sclerotized, slightly broader than long in middle; sub-genital plates separated by narrow membranous area covered with fine microtrichia, widened anteriorly to thomboidal, strongly edged sub-ostial membrane; anterior margin of sternum VIII with distinct triangular anteromedial projection; apophysis anterioris 2/3 length of segment VIII, curved before apex; colliculum large, sub-quadrangular, two times as broad as ductus bursae; ductus bursae narrow, about of even width; corpus bursae pear-shaped, about as long as ductus bursae, signum on the left side near entrance of corpus bursae, base small, distal hook long, narrow, nearly straight except for curved and pointed apical fifth. Diagnosis. The new species can be recognized externally by the rather contrasting, ereyish-black forewings with well-developed light brown pattern along veins and near dorsal margin. Gnorimoschema cinctipunctella (Erschoff, 1877) is more grey, the light brown pattern is usually less extensive, but some specimens look very similar. Gnorimoschema tabazhok is smaller in size (11.0-15.5 mm), more uniformly grey, black spots are less distinct. Gnorimoschema radkevichi Piskunov, 1980 is smaller in size (12.0— 14.0 mm) and has distinct black or light brown spot in the fold. The male genitalia are well recognizable by the sub-rectangular vincular process. Gnorimoschema bodillum Karsholt & Nielsen, 1974 is most similar to the new species regarding the male genitalia, but the vincular process is pointed, triangular rather than sub-rectangular, the sacculus is narrower, the vinculum is deeper emarginated medially and the phallus is narrower. The female genitalia are characterized by the strongly concave and well sclerotized anterior margin of sternum VIII in combination with unmodified sub-genital plate and narrow, straight with slightly curved apex of the signum. Gnorimoschema bodillum is similar but anterior margin of sternum VIII is less concave. Molecular data. BIN BOLD:ADF4416 (n=2). The intraspecific divergence of the barcode region is 0%. The distance to the nearest neighbour G. vastificum Braun, 1926 is 4.2% (p-dist). This distance is the proportion (p) of nucleotide sites at which A review of some new or little-known species of the genus Gnorimoschema ... 1B) Figures 20-25. Guorimoschema male genitalia 20 G. pamira sp. nov. — HT, Pamir (gen. slide 402/16, O. Bidzilya) 21 G. brachyptera sp. nov. — PT, Buryatia (gen. slide 159/16, O. Bidzilya) 22 G. brachyptera sp. nov. — PT, Buryatia (gen. slide 240/16, O. Bidzilya) 23 G. altaica sp. nov. — HT, Altai (gen. slide 31/18, O. Bidzilya) 24 G. tabazhok sp. nov. — PT, Altai (gen. slide 1250, P. Huemer) 25 G. tabazhok sp. nov. — PT, S Ural (gen. slide 43/18, O. Bidzilya) (gen. slide 43/18, O. Bidzilya). 114 Oleksiy Bidzilya et al. / ZooKeys 857: 105-138 (2019) two sequences being compared are different. It is obtained by dividing the number of nucleotide differences by the total number of nucleotides compared. It does not make any correction for multiple substitutions at the same site, substitution rate biases, or differences in evolutionary rates among sites. Distribution. Tadzhikistan (W Pamir). Biology. Host plant unknown. Adults were collected by light in late July at an elevation of 2800 m. The collecting site is the edge between a steep rocky slope and riverside sand dunes with plenty of Salix (Fig. 43). Etymology. The species name, a noun in apposition, reflects the distribution of the new species in the Pamir region of Tadzhikistan. Gnorimoschema brachyptera sp. nov. Figs 4—7, 21-22, 35-36 Material examined. Holotype. RUSSIA 9; S-Buryatia, Hamar Daban Mts., Murtoy Riv- er, Gusinoe ozero village 6 km NW; 51°11-13'N, 106°10-12'E; 700 m; forest steppe; 27 May 2006; K. Nupponen leg.; gen. slide 160/16, O. Bidzilya; TLMF Lep 21632; NUPP. Paratypes. 1 9, same data as for holotype; gen. slide 122/18, O. Bidzilya; TLMF Lep 21634; NUPP; 1 9, same data as for holotype; TLMF Lep 21633; NUPP; 1 3; same data as for holotype; gen. slide 159/16, O. Bidzilya; TLMF Lep 21636; NUPP; 1 &; same data as for holotype; gen. slide 240/16, O. Bidzilya; TLMF Lep 21635; NUPP; 1 3; Chita reg., 23 km N Kyra; 9 Aug. 1994; E. Ivanov leg.; gen. slide 90/15, O. Bidzilya; ZMKU; 1 3; same collecting data as for preceding; 10 Aug. 1994; P. Ustjuzhanin leg.; gen. slide 143/14, O. Bidzilya; ZMKU. Other material. RUSSIA 1 3; S-Buryatia, Hamar Daban Mnts., Murtoy River, Gusinoe ozero village 6 km NW; 51°11-13'N, 106°10-12'E, 700 m; forest steppe; 21 Jun. 2002; K. Nupponen leg.; gen. slide 194/16, O. Bidzilya; TLMF Lep 21645; NUPP. Description. Adult. Male (Figs 6, 7). Wingspan 12.8-13.5 mm. Head light grey, frons white; segment 2 of labial palpus white mixed with brown in distal half, inner surface white, with brush of modified scales on lower surface, segment III brown with white medial and apical rings, acute; scape brown with white apex, flagellum blackish- brown grey-ringed; thorax and tegulae covered with white brown-tipped scales; fore- wing brown, white oblique fascia from about 1/8 of costal margin to half length of the fold, diffuse white pattern in middle of cell, white broad subapical fascia on 3/4—4/5 length, paired black spots edged with brown in fold, small black prolonged spot mixed with brown in middle of cell, few black scales surrounded with brown in the corner of cell, fringe white, black-tipped; hindwing and fringe white. Variation. The paratype (gen. slide 240/16, O. Bidzilya) appears uniformly brown, white markings and black spots are indistinct (Fig. 7). Female (Figs 4, 5). Wingspan 11.1—-11.3 mm. As male, but hindwing shortened to 2/3—3/4 length of the forewing and stronger narrowed in apical 1/3, apical excavation less distinct, abdomen longer compared to male. A review of some new or little-known species of the genus Gnorimoschema ... 115 Figures 26-31. of Gnorimoschema male genitalia 26 G. tabazhok sp. nov. PT, Altai (gen. slide 416/16, O. Bidzilya) 27 G. tabazhok sp. nov. — PT, Tuva (gen. slide 319/16, O. Bidzilya) 28 G. tabazhok sp. nov. — PT, Altai (gen. slide GP 2_1.1.2019KN) 29 G. tabazhok sp. nov. — PT, Altai (gen. slide 19021, J. Sumpich) 30 G. yakovlevi sp. nov. — HT, Altai (gen. slide 406/16, O. Bidzilya) 31 G. yakovlevi sp. nov. — PT, Altai (gen. slide 1251, P. Huemer). 116 Oleksiy Bidzilya et al. / ZooKeys 857: 105-138 (2019) 0.5 mm Figures 32-33. Gnorimoschema male genitalia 32 G. kozlovi sp. nov. — HT, Mongolia (gen. slide 236/15, O. Bidzilya) 33 G radkevichi Pisk. — Altai (gen. slide 441/16, O. Bidzilya). Male genitalia (Figs 21, 22). Uncus sub-rectangular, apex triangular, pointed; gna- thos weakly curved, of even width, apex rounded; tegumen moderately broad, anterome- dial emargination triangular, extending to about half length of tegumen; valva broad at basal 1/3, then gradually curved, apex weakly widened, rounded; sacculus short, strongly broadened on base, distal portion narrow, curved inwards at right angle, gap to vincu- lar process narrow, triangular; vinculum broad, posterior margin with broad, shallow sub-triangular emargination, lateral process short, hump-shaped; saccus sub-triangular, gradually narrowed towards rounded or weakly pointed apex, usually not extended be- yond top of pedunculus; phallus narrow, straight, with needle-shaped, down-curved api- cal hook, group of short teeth before apex, caecum inflated, about 1/3 length of phallus. Variation. Valva varies in width; saccus extended beyond tip of pedunculus in some specimens. Female genitalia (Figs 35, 36). Papilla analis elongate, sub-triangular, densely cov- ered with short setae; apophysis posterioris 2.5—3 times longer than segment VII; segment VIII sub-quadrangular; subgenital plates medially strongly edged, separated with broadened posteriorly, membranous area covered with fine microtrichia, poste- rolateral sclerites sub-triangular, narrowly projecting anteromedially to the base of the apophysis anterioris, placed in middle of sternum VII; anterior margin of sternum VIII deeply concave, strongly sclerotized, medial opening distinct; apophysis ante- rioris about as long or slightly longer than segment VIII, straight; colliculum as long as broad; ductus bursae narrow, of even width, but inflated before colliculum; corpus bursae egg-shaped, about as long as ductus bursae, signum near entrance of corpus bursae, base elongated, distal hook weakly curved, apically narrowed. Diagnosis. The new species can be recognized externally by the contrasting, light grey forewing with black oblique fascia at 1/3, the distinct black markings edged with light brown in cell and in the fold and the white subapical fascia at 34. It resembles North European specimens of G. herbichii (Nowicki, 1864) (see Huemer and Karsholt 2010, pl. 1, fig. 2a—d) but the black markings are larger in G. brachyptera. The female A review of some new or little-known species of the genus Gnorimoschema ... Ly Figures 34-36. Gnorimoschema female genitalia 34 G. pamira sp. nov. — PT, Pamir (gen. slide 401/16, O. Bidzilya) 35 G. brachyptera sp. nov. — HT, Buryatia (gen. slide 160/16, O. Bidzilya) 36 G. brachyptera sp. nov. — PT, Buryatia (gen. slide 122/18, O. Bidzilya). 118 Oleksiy Bidzilya et al. / ZooKeys 857: 105-138 (2019) is well-defined by the brachypterous hindwings. The female of G. elbursicum Povol- ny, 1984 differs in the less contrasting, lighter, grey rather than brown forewing, the smaller size (8.2 mm) and the considerably narrower hindwing. The male genitalia are characterized by the sacculus, which is inflated on base with distal portion inwardly curved at right angle. Gnorimoschema fuscescens Li & Bidzilya, 2017 differs in the larger gap between the posterior margin of the vinculum and the distal portion of the sac- culus, and the valva with stronger inflated apex. Gnorimoschema steueri Povolny, 1975 differs by the longer sacculus, the shorter and broader saccus and the shorter phallus. The medially placed sub-triangular posterolateral sclerites in combination with the long apophysis anterioris (1.5 times longer than length of sternum VIII) and the short signum are characteristic for the female genitalia. Molecular data. BIN BOLD:ADF2846 (n=2), shared with G. yakovlevi. The mean intraspecific divergence of the barcode region is 0.15%. The distance to the nearest neighbour G. yakovlevi is 1.44% (p-dist). Distribution. Russia (Buryatia, Zabaikalskiy krai). Biology. Host plant unknown. Adults were collected in late May and August in dry steppe slopes with sparse vegetation (Fig. 44) at an elevation of 700-900 m. Etymology. ‘The species name, an adjective is derived from the Greek brachys, meaning short and the Greek ptéryx, meaning wing, referring to the shortened hind- wing, the most characteristic feature of this species. Remarks. An additional male from South Buryatia (gen. slide 194/16, O. Bidzilya) collected in June is larger (14.2 mm) and looks lighter and brighter, having more ex- tensive white pattern and well-developed orange-brown irroration around black spots. We have not found sufficient differences in the male genitalia between this specimen and additional males from the type-series. However, we decided to not include this specimen among the type-series due to the lack of females. Gnorimoschema altaica sp. nov. Figs 3395-23337 Material examined. Holotype. RUSSIA