Zoosyst. Evol. 96 (2) 2020, 723-745 | DOI 10.3897/zse.96.56097 Ate BERLIN First records of Pseudozeuxidae and Metapseudinae (Metapseudidae) (Crustacea, Tanaidacea) in Southwestern Atlantic, with descriptions of two new species Juliana Lopes Segadilha!, Cristiana Silveira Serejo! 1 Laboratorio de Carcinologia, Departamento de Invertebrados, Museu Nacional/Universidade Federal do Rio de Janeiro, Quinta da Boa Vista, 20940-040, Rio de Janeiro, RJ, Brazil 2 Programa de Pos-graduagdo em Ciéncias Bioldgicas (Zoologia) do Museu Nacional, Rio de Janeiro, Brazil http://zoobank. org/3B79D590-23A B-42EB-9ADD-2DEE0A17C4C7 Corresponding author: Juliana Lopes Segadilha (julianasegadilha@gmail.com) Academic editor: Sammy De Grave @ Received 2 July 2020 # Accepted 10 September 2020 @ Published 19 November 2020 Abstract Based on specimens collected from eulittoral zone in rocky shores of northeast of Rio de Janeiro (Brazil) on 2017, two new ta- naidaceans species from two different suborders are described: Apseudomorpha brasiliensis sp. nov. (Apseudomorpha, Metapseu- didae) and Pseudozeuxo fischeri sp. nov. (Tanaidomorpha, Pseudozeuxidae). Diagnostic characters of Apseudomorpha brasiliensis are mandible palp article-2 and article-3 with six and nine finely penicillate setae on inner margin, respectively; pereopod-1 carpus and propodus with two and four ventral spines, respectively; pleonites 2 and 5 with pleura having long distal seta; uropod exopod shorter than endopod articles 1-2 combined, endopod four-articled. Pseudozeuxo fischeri is characterized by pereopods 1-3 coxa with long seta about half as long as basis; pereopods 2-3 carpus with ventrodistal seta; propodus with two ventral setae; pereopods 4—6 propodus with two ventral spines and one seta; uropod endopod two- and exopod one-articled. This is the first record of the family Pseudozeuxidae and the metapseudid subfamily Metapseudinae from the Southwestern Atlantic (Brazil). Remarks on their associations with macroalgae and identification keys to world species of Apseudomorpha and Pseudozeuxo are provided. Key Words Apseudomorpha, Brazil, new record, Rio de Janeiro, rocky shores, Tanaidomorpha Introduction Gutu (1998) registered 29 species of the order Ta- naidacea from the Brazilian coast, 17 belonging to the Current knowledge about the crustacean order Tanaida- cea Dana, 1849 is still significantly underdeveloped, with the number of World species recognized likely to be an order of magnitude too low, with tanaidaceans potentially matching the orders Amphipoda and Isopoda in diversity mainly in environments as deep waters (Blazewicz-Pasz- kowycz et al. 2012). The Brazilian Tanaidacea fauna is still poorly known, although recent papers have revised upwards the number of recorded species 1n the past twen- ty years (Santos and Hansknecht 2007; Larsen et al. 2009; Santos et al. 2012; Aratjo-Silva et al. 2013; Segadilha et al. 2018; Segadilha et al. 2019). suborder Apseudomorpha Sieg, 1980 and 12 to the sub- order Tanaidomorpha Sieg, 1980. Larsen et al. (2009) up- dated Gutu’s catalog and listed 41 species in 31 genera and 12 families for the Brazilian area, of which 26 species belong to Apseudomorpha and 15 to Tanaidomorpha. In their latest work, Segadilha et al. (2019) registered a total of 54 species for Brazil. Nevertheless, the Brazilian ta- naidacean diversity 1s yet still relatively under-researched due to few specialists compared with other areas with a wider tradition of study of this group, such as Europe and United States, which makes evident the need for further studies along the Brazilian coast. Copyright Juliana Lopes Segadilha, Cristiana Silveira Serejo. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 724 Among coastal environments, rocky shores stand out for the high biodiversity of macroalgae, invertebrates, fish and seabirds, in addition to great ecological and economic im- portance (Messano et al. 2020). Rocky shore environments often cover large areas, although in Brazil they are almost ex- clusive to the southeast and south regions (Oigman-Pszczol et al. 2004), with the exception of the small rock formation present near the Cabo de Santo Agostinho, Pernambuco (Coutinho and Zalmon 2009). Thereby, the main part of the Brazilian coast where the rocky shores are among the most important ecosystems comprises Cabo Frio (Rio de Janeiro) to Cabo de Santa Marta (Sao Paulo) (Coutinho and Zalm- on 2009). The order Tanaidacea, among other Peracaridans, has great importance associated with secondary biological substrates in rocky shores due to its high density and diver- sity of species (Edgar and Moore 1986). The present study is part of the Project Rocky Shores: ecology, impacts, and conservation in the areas of Regiao dos Lagos and the north Fluminense, which aims to sur- vey biodiversity, study the abundance and structure of invertebrate macrofauna communities and their correla- tions with environmental parameters in rocky shores of the northern part of Rio de Janeiro (Macaé, Rio das Os- tras and Armacao dos Buzios). Material and methods The Project Rocky Shores: ecology, impacts, and conser- vation in the areas of Regiao dos Lagos and the north Fluminense has been executed by the Institute of Biodi- versity and Sustainability NUPEM/Universidade Federal do Rio de Janeiro with financial support of the Brazilian Biodiversity Fund (FUNBIO). Sampling was carried out at four stations: Areias Ne- gras Beach (AN) (22°31'48.98"S, 41°55'30.00"W) at Rio das Ostras; Cavaleiros Beach (CA) (22°24'17.67"S, 41°47'42.53"W) at Macaé; Calhetas Island, Santana Archipelago (AS) (22°23'54.46"S, 41°41'42.22"W), lo- cated in front of Macaé; and two points along the Rasa Beach (Bl and B2) (22°44'2.00"S, 41°57'27.36"W) (22°44'0.42"S, 41°57'26.42"W), at Armacao dos Buzios. At each site, collections were made from three zona- tion strata of the eulittoral zone: upper (A), intermediate (B) and lower (C). The strata were defined according to the dominant group of organisms covering each stratum, being: Upper = green macroalgae (Chlorophyta — Ulva, Enteromorpha, Chaetomorpha), bivalves (Brachidontes spp.) and small barnacles (Chthamalus spp.); Interme- diate = coralline macroalgae (Rhodophyta, e.g., Coral- lina, Cryptonemia, Gelidium, Jania) and large barnacles (Tetraclita stalactifera), and Lower = brown macroalgae (Ochrophyta, e.g., Sargassum, Dictyota). Five replicates were taken in each stratum, along 30 meters of extension on each shore. Sampling was made during low tides (< 0.2 m). The area of each sampled unit was delimited by a guadrat of 20x20 cm (0.04 m7) and samples were scraped with spatulas. zse.pensoft.net Segadilha, J.L., Serejo, C.S.: Pseudozeuxidae and Metapseudidae (Tanaidacea) from Brazil Specimens were sorted at the Laboratorio Integrado de Biologia de Vertebrados, located in NUPEM/UFRJ. The algae were previously dipped and slightly agitated in buck- ets with water to remove the largest number of attached or- ganisms, which were passed through a 1 mm mesh sieve. The material retained in the sieve (> 1mm) was separated, preserved in 70% ethanol and subsequently identified. The washed macroalgae and other larger organisms (e.g., sea urchins, bivalves) were carefully observed in stereomicro- scope to search for attached organisms, which were also included and identified. Drawings were made using a microscope Zeiss with a camera lucida and digitalized with WACOM Tablet using the program Adobe Illustrator CC 2017. The appendages were dissected using chemically sharpened tungsten-wire needles. Body length was measured from the tip of the rostrum to the tip of the pleotelson, and pereonite width at the broadest part. The length/width ratio was calcu- lated from the measurements made in the middle length and width of an article. The measurements of cheliped articles and the morphological terminology follow that used by Blazewicz-Paszkowycz (2007). The articulated protrusions on the distal edge of the maxillipedal en- dites are called ‘gustatory cusps’ as proposed by Sega- dilha et al. (2018) (equivalent to ‘cusps’ sensu Bird and Larsen (2009). The maps illustrating the distribution of the described species were prepared using Quantum GIS v.2.16.3 Software. All material cited herein (MNRJ 29854-29871) was stored at Museu Nacional/UFRJ and was saved during the fire in this institution in September 2018, being available for future analysis. Abbreviations L:W as long as wide MNRJ Museu Nacional/Universidade Federal do Rio de Janeiro, Brazil TBL total body length Stn station Results Four species of Tanaidacea belonging to four different families were identified in the material collected by the Project Rocky Shores in Rio de Janeiro (Brazil). One species was from the suborder Apseudomorpha (fam- ily Metapseudidae: Apseudomorpha_ brasiliensis sp. nov.) and three were from the suborder Tanaidomor- pha — two belonging to the superfamily Paratanaoidea (families Leptocheliidae Lang, 1973: Chondrochelia dubia (Kroyer, 1842) and Pseudozeuxidae Sieg, 1982: Pseudozeuxo fischeri sp. nov.), and one of the superfam- ily Tanaidoidea (family Tanaididae Nobili, 1906: Zeuxo coralensis Sieg, 1980). Herein we describe the two new species as seen below. Zoosyst. Evol. 96 (2) 2020, 723-745 Systematics Order Tanaidacea Dana, 1849 Suborder Apseudomorpha Sieg, 1980 Superfamily Apseudoidea Leach, 1814 Family Metapseudidae Lang, 1970 Subfamily Metapseudinae Lang, 1970 Genus Apseudomorpha Miller, 1940 http://zoobank.org/E6C 1 EC34-1A58-4426-B8D5-937138CDE171 Apseudomorpha Miller, 1940: 315. Apseudomorpha — Lang 1970: 603. — Gutu 1972: 303. — Gutu, 1981: 96, 103, 106. — Sieg 1986: 32. — Gutu 1987: 35, 38, 40. — Gutu 1991: 355. — Gutu 1996a: 85, 88. — Gutu 1996b: 138, 145. — Heard 2002: 373. — Heard et al. 2004: 55; 59. — Larsen 2005: 34. — Gutu 2006: 6, 16, 23, 26, 29, 36, 39, 41-44, 47-48, 120, 180-182, 190, 204. — Gutu 2007: 65. — Gutu 2009: 101, 103, 109— 111, 117. — Heard et al. 2009: 251. — Stepien and Blazewicz-Pasz- kowycz 2013: 560. — Heard et al. 2018: 302, 308 — Bird 2019: 68, Tab. 10 — Morales-Nufiez et al. 2019: 213-214, 227, 229-230, Tab. 2, Fig. 10. Diagnosis. See Morales-Ntfiez et al. 2019. Type species. Apseudomorpha oahuensis Miller, 1940. Species included. Apseudomorpha albida (Shiino, 1951); A. avicularia (Barnard, 1914); A. brasiliensis sp. nov.; A. drummi Morales-Nutfiez, Heard & Bird, 2019; A. fontainei Gutu, 1987; A. glebosa (Menzies, 1953); A. hirsuta (Stebbing, 1910); A. magdalenensis (Menzies, 1953); A. martinicana Gutu, 2009; A. negoescuae Gutu, 2007; A. oahuensis Miller, 1940; A. ortizi Gutu, 2006; A. timaruvia (Chilton, 1882); A. veleronis (Menzies, 1953); A. vestafricana Gutu, 2006. Remarks. Family Metapseudidae is divided into four subfamilies: Chondropodinae Gutu, 2008; Metapseudi- nae; Msangiinae Gutu, 2006 and Synapseudinae Gutu, 1972 (WoRMS 2020a). The genus Apseudomorpha is in- cluded within the subfamily Metapseudinae and can be distinguished from similar genera Pseudoapseudomor- pha Gutu, 1991 and Plectrocopus Gutu, 2006 mainly by the carapace with an acute and smoother rostrum and the absence of an exopod on the cheliped (Gutu 2006). Lang (1949) replaced the species Apseudes timaruvia Chilton, 1882 into the genus Apseudomorpha. According to Gutu (2009), although it resembles the Apseudomor- pha species by several features, it was wrongly included in this genus, mentioning differences as follows: (1) an- tennule with inner and outer flagellum with numberous segments (six and 14, respectively); (2) antenna flagellum with eight segments; (3) pereopod-2 propodus with two rows of spines; (4) cheliped propodus configuration; and (5) pleopods setation. Indeed Gutu’s conclusion was that Apseudes timaruvia belongs to an unknown genus (Gutu 2009). More recently, Graham Bird examined the speci- 120 mens of Apseudomorphatimaruviaand also stated that this species probably belongs in another genus as it differs from the other species of Apseudomorpha by (1) only pleonite-3 epimeron with a long apical seta; (2) more nu- merous antennular articles (six inner, nine outer; not 14 as Gutu indicated); and (3) pereopods 1-3 carpus and propodus with a double row of spines (Morales-Nufiez et al. 2019). The species Parapseudes hirsutus Stebbing, 1910 was reclassified by Sieg (1983) in the genus Apseudomorpha. However, Gutu (2009) indicated that the characteristics of the pereopods 1-3 (including the large number of the sternal spines on carpus and propodus), the great length of the antennule and the large number of the flagella segments, correspond to the genus Pseudoapseudomor- pha. Yet, due to the absence of the cheliped description in Stebbing (1910), more specifically if the exopodite is present or not, Gutu (2009) refrained from classification changes. Thus, both species mentioned are still retained in the genus Apseudomorpha pending its complete re-de- scription (G. J. Bird in prep.). The genus Apseudomorpha has a worldwide distribu- tion, with 15 species located in tropical and temperate regions of the Pacific, Atlantic and Indian Oceans (Mo- rales-Nufiez et al. 2019 and present study). Apseudomorpha brasiliensis sp. nov. http://zoobank.org/9EE09B 11-2A85-4550-A7AC-BE36F698BB68 Figures 1-7 Material examined. Holotype: Brazil + 1 2 ovigerous, TL 2.4mm (MNRJ 29863), Stn CR.AS. — C4B, 7 Sep. 2017, Santana Archipelago, Macaé, Rio de Janeiro, Brazil. Allotype: Brazil * 1 ¢, TL 1.8 mm (MNRJ 29864), Stn CR.AS. — C4B, 7 Sep. 2017, Santana Archipelago, Macaé, Rio de Janeiro, Brazil. Paratypes: Brazil * 1 2 ovigerous and 1 3, dissected, TL 2.0 mm (MNRJ 29857) and 1.9 mm (MNRJ 29858) respectively, Stn CR.AS. — C5B, 7 Sep. 2017, Santana Ar- chipelago, Macaé, Rio de Janeiro; Brazil * 5 9 non-ovig- erous (MNRJ 29859), same station; Brazil « 1 2 oviger- ous (MNRJ 29860), Stn CR.AS. — CIB, 25 Jun. 2017, Santana Archipelago, Macaé, Rio de Janeiro; Brazil * 1 9 non-ovigerous (MNRJ 29856), Stn CR.AS. — C2B, 7 Sep. 2017, Santana Archipelago, Macaé, Rio de Janei- ro; Brazil * 3 9 ovigerous, 3 2 non-ovigerous and 2 3 (MNRJ 29865), Stn CR.AS. — C4B, 7 Sep. 2017, Santana Archipelago, Macaé, Rio de Janeiro; Brazil * 3 9 ovig- erous (MNRJ 29862), Stn CR.AS. — C2C, 7 Sep. 2017, Santana Archipelago, Macaé, Rio de Janeiro; Brazil ¢ 1 9 non-ovigerous (MNRJ 29861), Stn CR.AS. — CSC, 7 Sep. 2017, Santana Archipelago, Macaé, Rio de Janeiro; Brazil * 6 2 non-ovigerous and 2 4 (MNRJ 29854), Stn CR.AS. — F3B, 25 Jun. 2017, Cavaleiros, Macaé, Rio de Janeiro; Brazil * 2 9 non-ovigerous (MNRJ 29855), Stn CR.AS. — F3C, 25 Jun. 2017, Santana Archipelago, Ma- caé, Rio de Janeiro. zse.pensoft.net 726 Segadilha, J.L., Serejo, C.S.: Pseudozeuxidae and Metapseudidae (Tanaidacea) from Brazil Diagnosis. Female. Rostrum with rounded tubercles at base and bifurcate tip. Pleonites 2 and 5 with pleura having long distal seta. Antennule article-1 inner mar- gin with one blunt apophysis, outer flagellum with three segments. Mandible palp article-2 and article-3 with six and nine finely penicillate setae on inner margin respec- tively. Maxilliped palp article-1 outer margin with seta. Pereopod-1 basis with dorso-proximal margin lacking blunt, spiniform process; carpus and propodus with two and four ventral spines, respectively. Pereopods 1—2 basis with several setae along ventral margin. Pleop- ods biramous, exopod and endopod each with one long penicillate seta. Uropod exopod shorter than endopod segments 1—2 combined, endopod with four segments. Male. Cheliped propodus just wider than long, ven- tral margin with only three simple setae (without proxi- mal apophysis). Description. Based on ovigerous 9 holotype (MNRJ 29863) and paratype (MNRJ 29857). Body (Figs 1A, 2A). Length 2.4 mm, about 4.8 times L:W. Cephalothorax (Fig. 2A) about 24% of TBL, shorter than pereonites 1—3 lengths combined, about 1.2 times L:W; rostrum convex with broad base, with rounded tu- bercles at base and median short bifurcate tip (Fig. 2B); eyelobes well defined, visual elements present. Carapace with simple seta near each ocular lobe (Fig. 2B). Pereon (Fig. 2A) about 60% of TBL, all pereonites wider than long, all with two simple setae on sub-distal dorsal margin and one seta on each anterolateral margins; pereonite-1 wider than others, rectangular with few min- ute setae on posterolateral margins; pereonites 2—5 lon- ger than pereonite-1; pereonite-6 shortest; pereonites 4—6 with weak anterolateral processes bearing setae. Figure 1. Digital image of Apseudomorpha brasiliensis sp. nov. Paratype female and allotype male (MNRJ 29865 and MNRJ 29864). Female, TL 2.4 mm; Male, TL 1.8 mm. Scale bar: 1.0 mm. zse.pensoft.net Zoosyst. Evol. 96 (2) 2020, 723-745 727 WY Ay Yi A B ws ~ Yd = Figure 2. Apseudomorpha brasiliensis sp. nov. Holotype, ovigerous female (MNRJ 29863). A Dorsal view; B enlargement of ros- trum; C enlargement of pleon and pleotelson. Scale bars: 1.0 mm for A and 0.1 mm for B—-C. zse.pensoft.net 728 Segadilha, J.L., Serejo, C.S.: Pseudozeuxidae and Metapseudidae (Tanaidacea) from Brazil Pleon (Figs 1A, 2A, C) about 16% of TBL, shorter than pereonites 1—2 lengths combined, about 1.2 times L:W; all pleonites sub-equal, wider than long, bearing pleop- ods; pleonite-1 laterally rounded (Fig. 2C); pleonites 2—5 prominent epimera with round tip (Fig 2C); pleonites 2 and 5 with long simple seta (Fig. 2C); pleonites 3—4 with small simple seta (Fig. 2C). Pleotelson (Figs 1A, 2A, C) as long as pereonite-6 (Fig. 2A); dorso-anteriorly with two tubercles, each with two simple setae (Fig. 2A, C); antero-laterally with tu- bercle with round tip and bearing simple distal seta (Fig. 2C); prominent triangular apex with two sub-distal setae (Fig. 2A, C). Antennule (Fig. 3A) shorter than cephalothorax. Pe- duncle with four articles. Article-1 2.2 times L:W; inner margin with two simple sub-distal setae and one blunt apophysis; outer margin with one distal pointed angle, with one simple and three penicillate setae proximally, with one long and one short simple and two penicillate setae medially, and with three simple setae distally. Ar- ticle-2 as long as wide; with seven simple and one peni- cillate setae distally. Article-3 just longer than wide; with two simple distal setae. Article-4 as long as wide; with two middle penicillate and one simple and one penicillate distal setae. Outer flagellum with three segments. Seg- ment-1 slightly longer than wide, with middle and two distal simple setae and one aesthetasc. Segment-2 about 1.3 times L:W; distally with three simple setae and one aesthetasc. Segment-3 2.5 times L:W, distally with five simple setae, one penicillate, and one aesthetasc. Inner flagellum with one segment, 2.8 times L:W, distally with three simple and two penicillate setae. Antenna (Fig. 3B) with eight articles. Article-1 inner margin with rounded distal process. Article-2 inner mar- gin with penicillate seta and distal triangular apophysis; outer margin scaly with two penicillate setae; squama present, longer than article-3, with two distal simple setae. Article-3 wider than long; inner sub-distal margin with simple seta and apophysis. Article-4 inner distal margin with two penicillate setae. Article-5 about 1.3 times L:W, longer than article-4, with two simple and six penicillate setae. Article-6 half-length of article-5, with two simple distal setae. Article-7 longer than article-6, inner distal margin with two simple setae; outer distal margin with simple seta. Article-8 shortest, with three simple and two penicillate distal setae. Mouthparts: Labrum not recovered. Mandibles (Fig. 3C—D). Right mandible incisor with four denti- cles (Fig. 3C); lacinia mobilis with several denticles on dorsal margin (Fig. 3C). Left mandible incisor with two denticles (Fig. 3D); /acinia mobilis broad with three den- ticles (Fig. 3D); setiferous lobe with four multi-furcate setae (Fig. 3D). Molar process of both mandibles dam- aged during dissection. Palp (Fig. 3D) article-1 shortest, inner margin with distal simple seta; article-2 longest, 1.8 times L:W, mid-inner margin with six setulate setae; article-3 with nine setulate inner setae. zse.pensoft.net Maxillule (Fig. 3E). Inner endite not recovered. Outer endite with ten (one setulose) distal and two sub-distal spines, margins finely setose; palp biarticulate with three distal setae. Maxilla (Fig. 4A). Margins finely setose; outer lobe of moveable endite with six setae; inner lobe of moveable endite with six setae; outer lobe of fixed endite with eleven and three plumose setae (Fig. 4A); inner lobe of fixed en- dite with eleven setae. Labium and epignath not recovered. Maxilliped (Fig. 4B—C). Coxa short and wide. Basis finely setose with microtrichia, wider than long, with two denticles on outer distal margin. Palp article-1 shortest, distal inner margin with long (passing article-3) simple seta, and distal outer margin expanded distally with sim- ple seta; article-2 longest, inner margin finely setulose proximally, with 14 simple setae, outer distal margin with strong spine; article-3 inner margin with eight simple se- tae; article-4 with six simple inner setae and two subdistal outer setae. Endite (Fig. 4B—C) with inner margin with seven basally-swollen setulate setae, four simple setae and four apically long simple setae; outer margin setulose with small denticles on mid margin. Cheliped (Fig. 5A). Basis, 1.5 times L:W; ventral mar- gin with distal simple seta. Merus triangular; ventral mar- gin with three simple setae sub-distally. Carpus 2.7 times L:W, just longer than basis, widest distally; ventral margin with one middle and two sub-distal simple setae; dorsal margin with middle and sub-distal simple setae. Propodus 1.9 times L:W; with two simple setae near articulation of dactylus (one long and one small); fixed finger with three ventral simple setae, with five sub-marginal simple setae on outer incisive margin; inner face with sub-distal and mid-ventral simple setae. Dactylus and unguis slightly longer than fixed finger; inner face with simple seta on ventral margin. Pereopod-1 (Fig. 6A). Coxa with two penicillate and four minute setae. Basis 3.6 times L:W; ventral margin with three penicillate and three simple setae, and one spine, one penicillate and one long seta distally; dorsal margin with seven penicillate setae. Ischitum with penicil- late and simple ventral seta. Merus 1.7 times L: W, widest distally; ventral margin with two simple setae and one sub-distal serrulate spine; outer margin with two middle setulate setae; distodorsal margin with simple seta and serrulate spine. Carpus as long as wide, widest distally, ventral margin with two simple setae and two serrulate spines; outer margin with middle setulate setae; distodor- sal margin with penicillate, two simple setae and serru- late spine. Propodus 2.5 times L:W, ventral margin with four serrulate spines and simple seta; outer margin with middle setulate setae; dorsal margin with two penicillate, simple seta and two (one sub-distal and one distal) serru- late spines, and one sub-distal simple seta; inner face with long penicillate and two pectinate setae distally. Dactylus together with unguis shorter than propodus, dactylus lon- ger than unguis; dactylus with two minute ventral setae and small sub-distal denticle. Unguis curved. Zoosyst. Evol. 96 (2) 2020, 723-745 729 Figure 3. Apseudomorpha brasiliensis sp. nov. Paratype, ovigerous female dissected (MNRJ 29857). A Antennule; B antenna; C right mandible; D left mandible; E maxillule. Scale bars: 0.1 mm. zse.pensoft.net 730 Segadilha, J.L., Serejo, C.S.: Pseudozeuxidae and Metapseudidae (Tanaidacea) from Brazil er wy \ Figure 4. Apseudomorpha brasiliensis sp. nov. Paratype, ovigerous female dissected (MNRJ 29857). A Maxilla; B maxilliped; C endite of maxilliped. Scale bar: 0.1 mm. Pereopod-2 (Fig. 6B) shorter and more gracile than pereopod-1. Coxa with two simple setae. Basis 3.8 times L:W; ventral margin with four simple setae and one long seta distally; dorsal margin with four sim- ple setae. Ischium wider than long, with simple ven- tral seta. Merus 1.7 times L:W, widest distally; ventral margin with two simple setae and two sub-distal ser- rulate spines; outer margin with middle simple seta; distodorsal margin with simple seta. Carpus about as long as wide; ventral margin with four serrulate spines; distodorsal margin with four simple setae and two ser- rulate spines. Propodus 2.6 times L:W; ventral margin with four serrulate spines and two simple setae; dorsal margin with penicillate and two (one sub-distal and one distal) serrulate spines, and one sub-distal simple seta (longer than dactylus); inner face with two pectinate se- tae distally. Dactylus together with unguis shorter than propodus, dactylus longer than unguis; dactylus with two (one in the middle and one sub-distal) ventral setae and small sub-distal denticle. Unguis curved. Pereopod-3 (Fig. 6C) similar to pereopod-2 but short- er. Coxa seta broken (as in pereopod-4). Basis ventral margin with two penicillate and one simple setae, and one stout seta distally; dorsal margin with penicillate and zse.pensoft.net two simple setae. Ischium with two ventral setae. Merus shorter. Carpus with only two distodorsal setae. Pereopod-4 (Fig. 6D). Coxa with simple seta. Basis three times L:W; ventral margin with two simple and one distal setae; dorsal margin with two penicillate and three simple setae. Ischium wider than long, with two ventral setae. Merus 1.5 times L:W; widest distally; ventral mar- gin with sub-distal simple seta and spine; outer margin with middle setulate seta; distodorsal margin with sim- ple seta. Carpus 1.6 times L:W; ventral margin with two simple setae and four spines; distodorsal margin with five simple setae. Propodus 2.3 times L:W; ventral margin with two spines; outer margin with middle setulate seta; dorsal margin with penicillate seta, and sub-distal spine and simple seta; inner face with row of five sub-distal pectinate plus one long simple setae distally. Dactylus to- gether with unguis shorter than propodus, dactylus longer than unguis; dactylus with two (one in the middle and one sub-distal) ventral setae and small sub-distal denticle. Unguis curved. Pereopod-5 (Fig. 6E) similar to pereopod-4. Pereopod-6 (Fig. 6F) similar to pereopod-5 but slight- ly smaller. Basis dorsal margin with two penicillate and two simple setae. Ischium with ventral setae. Merus Zoosyst. Evol. 96 (2) 2020, 723-745 731 Figure 5. Apseudomorpha brasiliensis sp. nov. Paratype, ovigerous female (MNRJ 29857) and male (MNRJ 29858) dissected. A Cheliped female; B cheliped male. Scale bar: 0.1 mm. zse.pensoft.net 732 ventral margin with sub-distal simple seta; outer margin with middle setulate seta; distodorsal margin with simple seta. Propodus ventral margin with two spines and sim- ple seta; outer margin with middle setulate seta; dorsal margin with penicillate seta, and sub-distal spine and two simple setae; distally with row of five pectinate and two simple setae. Pleopods (Fig. 7A). Five similar, biramous pairs. Bas- al article 5.8 times L:W, 2.4 times longer than both rami, naked. Exopod slightly longer than endopod, both uniar- ticulate and with long plumose seta distally. Uropod (Fig. 7B) biramous. Basal article 1.8 times L:W; inner margin with long distal simple seta; outer margin with one mid and two distal simple setae (one long and one small). Exopod of two segments, shorter than endopod segments 1—2 combined. Segment-1 with two simple distal setae. Segment-2 with two simple distal setae. Endopod shorter than pleon and pleotelson com- bined, with four segments. Segment-1 with two peni- cillate and one simple distal setae. Segments 2-4 about same length. Segment-2 with two penicillate and three simple setae on distal margin. Segment-3 with two peni- cillate and two simple distal setae. Terminal segment with one penicillate and four simple setae distally. Adult male. Length 1.8 mm. Similar to females except in the size of the chelipeds (allotype and paratype: MNRJ 29864, MNRJ 29858, respectively). Cheliped (Fig. 5B) robust. Basis longer than wide, with one distoventral and two proximal simple setae. Merus with middle and two distoventral simple setae. Carpus broad and short; distoventral margin with a pro- longation and three proximal setae. Propodus just wider than long; ventral margin with three simple setae; fixed finger short, with ten sub-marginal simple setae on out- er incisive margin; dorsal margin with two denticles and grasping edge, claw short. Dactylus curved, ventral mar- gin with mid-blunt apophysis and grasping edge with row of five thin spines. Etymology. The name is dedicated to Brazil, the country where the species were collected. Type locality. Eulittoral zone of rocky shores at Santana Archipelago, Macaé, Rio de Janeiro, Brazil. Distribution. This species was found exclusively at eulit- toral zone on rocky shores (macroalgae bank) of Santana Archipelago, Macaé, Rio de Janeiro, Brazil, Southwest- ern Atlantic (Fig. 13). Ecology. In total, 33 specimens of Apseudomorpha brasiliensis sp. nov. were found in eight of 60 quadrats (13.3%) collected at Santana Archipelago. This species was most abundant in the intermediate stratum (82%), however it was also found in the lower stratum (18%), being absent in the upper stratum. The substrate of the quadrats where the species was found was covered pre- dominantly by articulated calcareous algae (Rhodophy- zse.pensoft.net Segadilha, J.L., Serejo, C.S.: Pseudozeuxidae and Metapseudidae (Tanaidacea) from Brazil ta), covering a surface between 50-95% (average of 81% of the area). The intermediate stratum presented mainly Rhodophyta macroalgae, although brown algae Ochro- phyta also occurred. The predominant Rhodophyta gen- era were Corallina, Jania and Arthrocardia, with other taxa such as Gracilaria, Hypnea, Pterocladiella and Plo- camium. The lower stratum showed a greater coverage of brown algae, mainly of the genera Sargassum, Padina and Colpomenia. Remarks. The new species from Brazil is more similar to those Apseudomorpha species characterized by having long setae only on the second and fifth pleonites epimera, namely A. drummi, A. fontainei, A. glebosa, and A. mar- tinicana (Morales-Nufiez et al. 2019). Apseudomorpha brasiliensis sp. nov. differs from A. fontainei by having (1) mandibular palp article-1 with one inner simple distal seta (two in A. fontainei); (2) pereopods 4—5 carpus dist- odorsal long seta (longer than half of propodus); (3) bira- mous pleopods (uniramous in A. fontainei); and (4) uro- pod endopod with four segments (seven in A. fontainei). Apseudomorpha brasiliensis is different from A. glebo- sa by (1) pereopod-1 carpus and propodus ventral margin with two and four spines, respectively (three and five in A. glebosa); (2) pereopod-6 with two ventral spines (four in A. glebosa); and (3) each pleopod rami with long pen- icillate seta (only one rami with two setae in A. glebosa). The new species is distinguished from A. martinicana (1) antennule article-1 inner margin with one blunt apophysis (3-4 apophyses in A. martinicana), (2) antennule main flagellum with three segments (four in A. martinicana); (3) mandible palp with article-2 with six inner penicillate setae (three in A. martinicana), and (4) pereopods 2-3 propodus with four ventral spines (three in A. martinica). The new species from Brazil closely resembles A. drummi by having antennule main flagellum with three segments, pereopod-1 carpus and propodus with two and four ventral spines respectively and pleopods with each rami having one long penicillate seta. The former, howev- er, can be distinguished from A. drummi by: (1) antennule article-1 inner margin with one blunt apophysis (three apophyses in A. drummi); (2) mandible palp article-2 and article-3 with six and nine finely penicillate setae on inner margin respectively (five and eight in A. drummi), (3) pereopods 1—2 basis with several setae along ventral margin, except distally (maximum one in A. drummi); (4) uropod exopod shorter than endopod segments 1—2 combined (longer in A. drummi); and (5) uropod endopod with four segments (five in A. drummi). These compari- sons and others are developed in a key to the species of Apseudomorpha, shown below. Apseudomorpha brasiliensis is the first record of the subfamily Metapseudinae from the Southwestern Atlan- tic. Including the present data, the family Metapseudidae is now represented by four genera in three subfamilies in Brazil: Chondropodinae (Calozodion Gardiner, 1973 and Vestigiramus Gutu, 2009), Metapseudinae (Apseudomor- pha) and Synapseudinae (Synapseudes Miller, 1940). Zoosyst. Evol. 96 (2) 2020, 723-745 733 Figure 6. Apseudomorpha brasiliensis sp. nov. Paratype, ovigerous female dissected (MNRJ 29857). A—F Pereopods 1-6. Scale bar: 0.1 mm. zse.pensoft.net 734 Segadilha, J.L., Serejo, C.S.: Pseudozeuxidae and Metapseudidae (Tanaidacea) from Brazil Figure 7. Apseudomorpha brasiliensis sp. nov. Paratype, ovigerous female dissected (MNRJ 29857). A Pleopod; B uropod. Scale bars: 0.1 mm. Key to the known species (females) of Apseudomorpha (modified from Gutu 2009 and Morales- Nufiez et al. 2019): Pereopod-1 basis dorso-proximal margin with blunt, spiniform process....A. negoescuae [Indian Ocean: Réunion Island] Pereopod-1 basis dorso-proximal margin lacking blunt, SpiNifOrmM PrOCeSs ...............cceccsecceccececeeceecsetacsceeececcerseneseeneees 2 Pleonites 1-5 without a long seta on dorsolateral margins ......... A. avicularia [Southeastern Atlantic Ocean: Cape Town] Atleast the last pleanite:with a long séta-on :dorsolaterall Margin sis A. asiveccecees ss ccrsacsnes neces te texiti pr ve are nnseeeeeeaeisarermense ses 3 ap) 2) O) O/C bse | 251-1 | Ae Oe OR Scr TR, Vt SONOS A TAL SO, A. vestafricana [Eastern Atlantic Ocean: Mauritania] PRES DOCS ore Se tri OU NM MIT HOU SO ca sak wet Si a dat le Mea Atal ate Mabe de OEP A hE te Pi du Rl Steet ALE ein ket LNs i. Sta 4 Onilvtie last: pleonite withia lone seta-omelistalateral mares. cs ated esc ecns rots treeatenedee cet yeseatseredereersesnareebeeeee 5 Mere therm the last: pleanite witht a: lene Setaon-cistolAleraliiiar elise. nat caets, bith taamceeeer tse pense wer ees arian ee ane 6 Antennule first peduncular article with some dentiform processes on the proximal half of inner margin............ccccceeee ee deterrents amtes. named gees) alee shame ad eee een o srteel aes 23: 81-108. Gutu M (1987) Apseudomorpha fontainei new species of Tanaidacea (Crustacea from the Red Sea. Travaux du Muséum National d’ His- toire Naturelle 29: 35-41. Gutu M (1991) The description of a new genus and of two new spe- cies of Tanaidacea (Crustacea) from Western Indian Ocean. Travaux du Muséum National d’Histoire Naturelle 31: 355-364. zse.pensoft.net Gutu M (1996a) Tanaidaceans (Crustacea, Peracarida) from Brazil, with description of new taxa and systematical remarks on some families. Travaux du Muséum National d’Histoire Naturelle 36: 23-13. Gutu M (1996b) The synoptic table and key to superspecific taxa of re- cent Apseudomorpha (Crustacea, Tanaidacea). Travaux du Muséum National d’ Histoire Naturelle 36: 135-146. Gutu M (1998) Malacostraca—Peracarida. Tanaidacea. In: Young PS (Ed.) Catalogue of Crustacea of Brazil. Museu Nacional, Rio de Ja- neiro, 549-557. Gutu M (2006) New Apseudomorph taxa (Crustacea, Tanaidacea) of the World Ocean. Curtea Vec, Bucharest, 318 pp. Gutu M (2007) Contribution to the knowledge of the Indo-West-Pacific Apseudomorpha (Crustacea: Tanaidacea). Travaux du Muséum Na- tional d’Histoire Naturelle 50: 47-86. Gutu M (2009) A contribution to the knowledge of Metapseudids. De- scription of a new genus and three new species from the Caribbean Sea and the Indian Ocean (Crustacea: Tanaidacea: Apseudomorpha). Travaux du Muséum National d’Histoire Naturelle 52: 101-125. Heard RW (2002) 1 Contributions to the Study of East Pacific Crusta- ceans Annotated checklist and bibliography for the order Tanaida- cea (Crustacea: Malacostraca: Peracarida) reported from the Pacific coasts of the Americas (Alaska to Chile) and associated off shore island, 369-383. Heard RW, Breedy O, Vargas R (2009) Tanaidaceans. In: Wehrtmann JC (Ed.) Marine Biodiversity of Costa Rica, Central America. 245—256. https://doi.org/10.1007/978-1-4020-8278-8 22 Heard RW, Hansknecht T, Larsen K (2004) An illustrated identification guide to Florida Tanaidacea (Crustacea: Peracarida) occurring in depths of less than 200 m. State of Florida, Department of Environ- mental Protection, Tallahassee, 92 pp. Heard RW, Stepien A, Drumm DT, Blazewicz M, Anderson G (2018) Systematic and taxonomic observations on the subfamily Syn- apseudinae Gutu, 1972 and related metapseudid taxa (Crustacea: Tanaidacea: Apseudomorpha), with the erection of a new genus and descriptions of three new species. Zootaxa 4370: 301-344. https:// doi.org/10.11646/zootaxa.4370.4.1 Kakui K, Fujita Y (2018) Haimormus shimojiensis, a new genus and species of Pseudozeuxidae (Crustacea: Tanaidacea) from a subma- rine limestone cave in Northwestern Pacific. PeerJ 6:e4720: 1-15. https://doi.org/10.7717/peerj.4720 Lang K (1949) Contribution to the systematics and synonymics of the Tanaidacea. Arkiv for Zoologie, Series 1 42: 1-14. Lang K (1970) Taxonomische und phylogenetische Untersuchun- gen uber die Tanaidaceen. 5. Die Gattung Typhlotanais G.O. Sars, 1882, nebst Beschreibung einer neuen Art dieser Gattung. Dazu eine Berichtigung der Dornenzahl des Enditen der Maxillulae bei Typhlotanais peculiaris Lang, 1968. Arkiv for Zoologie 2: 267-291. Larsen K (2005) Deep-Sea Tanaidacea (Peracarida) from the Gulf of Mexico. Brill, Leiden, Boston, 381 pp. Larsen K, Araujo-Silva CL, Coelho PA (2009) Tanaidacea from Brazil. I. The family Tanaellidae Larsen & Wilson, 2002. Zootaxa 2141: 1-19. https://do1.org/10.11646/zootaxa.2141.1.1 Larsen K, Wilson GDF (2002) Tanaidacean phylogeny, the first step: The superfamily Paratanaidoidea. Journal of Zoological Systemat- ics and Evolutionary Research 40: 205-222. https://doi.org/10.1046/ j.1439-0469.2002.00193.x Zoosyst. Evol. 96 (2) 2020, 723-745 Masunari S (1983) Postmarsupial development and population dynam- ics of Leptochelia savignyi (Kroyer, 1842) (Tanaidacea). Crustacea- na 44: 151-162. https://doi.org/10.1163/156854083 X00776 Messano LVR de, Gongalves JEA, Ferreira CEL, Coutinho R (2020) Caracteriza¢ao ecolodgica dos ambientes de substrato consolidado. In: Baptista D, Granthom-Costa LV, Coutinho R (Eds) Biodiversi- dade Marinha dos costées rochosos de Arraial do Cabo: Historico, Ecologia e Conservacao. IEAPM, Rio de Janeiro: 48-75. Miller MA (1940) The Isopod Crustacea of the Hawaiian Islands (Che- lifera and Valvifera). Bernice Pauahi Bishop Museum Occasional Papers 15: 295-319. Morales-Nufiez AG, Heard RW, Bird GJ (2019) Two new apseudomor- phan species (Crustacea: Tanaidacea: Metapseudidae) from Mo‘orea Island (Society Islands, French Polynesia) with taxonomic keys. Zootaxa 4564: 1—213. https://doi.org/10.11646/zootaxa.4564.1.8 Oigman-Pszczol SS, Figueiredo MA de O, Creed JC (2004) Distribution of Benthic Communities on the Tropical Rocky Subtidal of Armacao dos Buzios, Southeastern Brazil. Marine Ecology 25: 173-190. https://doi.org/10.1111/j.1439-0485.2004.00018.x Pires AMS (1980) Ecological Studies on Intertidal and Infralitto- ral Brazilian Tanaidacea (Crustacea, Peracarida). Studies on Neotropical Fauna and Environment 15: 141-153. https://do1. org/10.1080/0165052800936057 1 Riggio S (2008) Synapseudes shiinoi Riggio, 1973, a species of Ta- naidacea found in the Mediterranean. Crustaceana 33: 153-162. https://doi.org/10.1163/156854077x00052 Santos KC dos, Hansknecht T (2007) Taraxapseudes n. gen., Taraxa- pseudes diversus (Lang, 1968) n. comb. and two new species of At- lantapseudes Bacescu, 1978 (Tanaidacea: Apseudidae) from Brazil and Madagascar, with a key for the genus. Zootaxa 1639: 23-39. https://doi.org/10.11646/zootaxa.1639.1.2 Santos KC dos, Santana W, Pires-Vanin AMS (2012) Postispinatus youngi n. gen., n. sp. (Apseudomorpha; Tanaidacea; Crustacea): 745 Phylogenetic analysis and taxonomic remarks about kalliapseudids. Zootaxa 3192: 24-38. https://doi.org/10.11646/zootaxa.3192.1.2 Segadilha JL, Gellert M, Blazewicz M (2018) A new genus of Tanaidacea (Peracarida, Typhlotanaidae) from the Atlantic slope. Marine Biodi- versity 48: 915-925. https://doi.org/10.1007/s12526-018-0856-y Segadilha JL, Serejo CS, Blazewicz M (2019) New species of Typhlota- naidae (Crustacea, Tanaidacea) from the Brazilian coast: genera Hamatipeda, Meromonakantha and Paratyphlotanais, with descrip- tion of Targaryenella gen. nov. Zootaxa 4661: 309-342. https://doi. org/10.11646/zootaxa.4661.2.4 Sieg J (1982) Uber Ein “Connecting Link” in Der Phylogenie Der Tanaidomorpha (Tanaidacea). Crustaceana 43: 65-77. https://doi. org/10.1163/156854082 X00092 Sieg J (1983) Tanaidacea. In: Gruner H-E, Holthuis LB (Eds) Crusta- ceorum Catalogus. The Hague, 552 pp. Sieg J (1986) Crustacea Tanaidacea of the Antarctic and the Subant- arctic — 1. On material collected at Tierra del Fuego, Isla de los Es- tados, and the west coast of the Antarctic Peninsula. In: Kornicker LS (Ed.) Biology of the Antarctic Seas 18 (Vol. 45). Washington, 180 pp. https://doi.org/10.1029/AR045 Stebbing TRR (1910) No. VI.- Isopoda from the Indian Ocean and British East Africa. The Percy Sladen Trust Expedition to the In- dian Ocean in 1905 (Vol. III). The Transactions of the Linne- an Society of London, Series 2, Zoology 14: 83-122. https://do1. org/10.1111/j.1096-3642.1910.tb00525.x Stepien A, Blazewicz M (2013) Four new species and two new gen- era of Metapseudidae (Crustacea: Tanaidacea: Apseudomorpha) from Australian coral reefs. Zootaxa 3717: 559-592. https://doi. org/10.11646/zootaxa.3717.4.7 WoRMS (2020a) Metapseudidae Lang, 1970. http://www.marinespecies. org/aphia. php? p=taxdetails&id=136155 [Accessed 12 August 2020] WoRMS (2020b) Pseudozeuxo Sieg, 1982. http://www.marinespecies. org/aphia. php? p=taxdetails&id=247008 [Accessed 12 August 2020] zse.pensoft.net