CompCytogen 14(4): 639-643 (2020) COMPARATIVE — *reerrerewet open-access oven

doi: 10.3897/CompCytogen.v | 4i4.56080 Kas Cyto genetics

http://com pcytogen.pensoft. net International Journal of Plant & Animal Cytogenetics,

Karyosystematics, and Molecular Systematics

Corrigenda: Cytogenetic markers as a tool for
characterization of hybrids of Astyanax Baird &
Girard, 1854 and Hyphessobrycon Eigenmann, 1907.
Comparative Cytogenetics 14(2): 231-242. https://doi.
org/10.3897/CompCytogen.vl4i2.49513

Caio Augusto Gomes Goes', Sandro Natal Daniel', Lucas Henrique Piva’,
George Shigueki Yasui’, Roberto Ferreira Artoni®, Diogo Teruo Hashimoto’,
Fausto Foresti?, Fabio Porto-Foresti'!

| Universidade Estadual Paulista (UNESP) “filio de Mesquita Filho”, Faculdade de Ciéncias, Edmundo
Carrijo Coube Avenue, Bauru, SP Brazil 2 Centro nacional de Pesquisa e Conservagéo da Biota Aquatica
Continental (CEPTA-ICMBIO), Prefeito Euberto Nemésio Pereira Godéi Highway, Pirassununga, SP. Brazil
3 Universidade Estadual de Ponta Grossa, Setor de Ciéncias Biolégicas e da Satuide, Santos Andrade Square,
Ponta Grossa, PR, Brazil 4 Universidade Estadual Paulista (UNESP) “filio de Mesquita Filho”, Centro de
Aquicultura da UNESP Prof: Paulo Donato Castelane Acess way, Jaboticabal, SR Brazil 5 Universidade
Estadual Paulista “Julio de Mesquita Filho” (UNESP), Instituto de Biociéncias, Prof. Montenegro Avenue,
Botucatu, SP Brazil

Corresponding author: Fabio Porto-Foresti (fpforesti@fc.unesp.br)

Academic editor: Inna Kuznetsova | Received 1 July 2020 | Accepted 7 August 2020 | Published 29 December 2020
http://zoobank.org/60AD5B7B-0154-4EED-86FE-8C57E 1 12B602

Citation: Goes CAG, Daniel SN, Piva LH, Yasui GS, Artoni RF, Hashimoto DT, Foresti FE Porto-Foresti F (2020)
Corrigenda: Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and
Hyphessobrycon Eigenmann, 1907. Comparative Cytogenetics 14(2): 231-242. https://doi.org/10.3897/CompCytogen.
v14i2.49513. Comparative Cytogenetics 14(4): 639-643. https://doi.org/10.3897/CompCytogen.v14i4.56080

After the publication of our article, we detected some inconsistencies in figures and
figure captions. The nominal species Astyanax altiparanae was recently recognized as a
new junior synonym of Astyanax lacustris Litken, 1875. Thus, we corrected this issue
in all figures. Captions of figure 3, 5 and 6 were also incorrect. Corrected figures and
captions are as follows:

Copyright Caio Augusto Gomes Goes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License
(CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

640 Caio Augusto Gomes Goes et al. / Comparative Cytogenetics 14(4): 639-643 (2020)

A. lacustris

Ke Wn Xa AAAK BY AD Cine GAA BA AAGA Ga an 68 46AA BAAN AMAG mower
41 2 3 4 5 6 7 8 9 10 11 12 13. 14 15° 8 419 22 23 24 25

16 17 1 20 21
a

m sm st
H. anisitsi

98 anne nn uu ROOD 08 56 AB UR AE OR oy te ne AR AR 00 On 08 Ad 08 aa nn
4 2 3 4 5 6 ZT 9 10 1 12

8 13 1415 16 17 18 19 20 21 22 23 24 25
m sm st a
A. fasciatus

BR a8 uc os ax 8643 6d an oa an OF BS 06 05 08 an 88 ba on we 00 06 a0
4 2 3 4 5 6 ve 8 i] 10 411 12 413 14 15 16 17 18 419 20 21 22 23 24

m sm st a

A, sehubarti

a8 aX a8 Bx is i it &u a3 ax BEAR EAAR KS OM BA Oo

10 12 13 14 15 16 17
a

m

Figure |. Karyotypes of parental individuals analyzed: A. Jacustris (3m+6sm+7st+9a), H. anisitsi
(Sm+1sm+10st+9a), A. fasciatus (5m+6sm+6st+7a) and A. schubarti (5m+5sm+5st+3a). Scale bar: 5um.

A. lacustris x H. anisitsi

Bx axes BKAAxaAA BEAKAADAKKEBGAAKS NAAOOHARALOHOARARA
1.2 3 4 6 7 8 § 10 41 42 43 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 3334 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
si

A. lacustris x A. fasciatus

CCanssau GORUTECE Ed cn OhEbamenagece GARDAR seater nese

12 3 4 5 6 7 8 9 10 1 12 13 14 15 16 17 18 19 20 _21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49
= = m 7 ea ee ieee | a

m
A. lacustris x A, schubarti

Bekssn. Gaatanansan HEAAGEGAGEAAAARAARHAOAAA

12093 4 5 6 7 8 9 10 11 12 13 14 15 1617 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43
m sm st a

Spm

Figure 2. Karyotypes ofhybrid products of the genus Astyanax: A. lacustris x H. anisitsi(8m+7sm+17st+18a),
A. lacustris x A. fasciatus (8m+7sm+17st+18a) and A. lacustris x A. schubarti (8m+11sm+12st+12a), re-
spectively. Scale bar: 5um.

641

Hybridization between genders in fish

Figure 3. Heterochromatic markers obtained by C-banding on metaphase plates of A. lacustris (a, d, g),
A, fasciatus (b), A. schubarti (e), H. anisitsi (h) and hybrids A. lacustris x A. fasciatus (€), A. lacustris x
A. schubarti (f) and A. lacustris x H. anisitsi (i) after C-banding. The arrows indicate heterochromatic
blocks. The chromosomes number of A. /acustris and H. anisitsi hybrid is 2N = 51, the metaphase plate of
A. lacustris and. H. anisitsi hybrid (i) contains a heterochromatic chromosome (arrowhead). Scale bar: 5um.

642 Caio Augusto Gomes Goes et al. / Comparative Cytogenetics 14(4): 639-643 (2020)

A. lacustris A. schubarti F1 A. lacustris x A. schubarti

Figure 4. FISH with probes 5S (green) and 18S (red). The results are labeled as: A. lacustris (a, d, g), A. schu-
barti (b), hybrid A. lacustris x A. schubarti (€), A. fasciatus (e), hybrid A. lacustris x A. fasciatus (f), H. anisitsi
(h), and hybrid A. dacustris x H. anisitsi (i). The arrows indicate chromosomes inherited from A. /acustris, and

the arrowheads indicate chromosomes inherited from the other respective parents. Scale bar: 5m.

Hybridization between genders in fish 643

A. lacustris x H. anisitsi

1 2 3 4 &§ 6 FT 9 10 11 12 13 14 15 16 17 #18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33. (34 «(35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
sm st a

A. lacustris x A. fasciatus
4 5 6 7 8 9 1612 13 14 «15 22 23 24 25 26 27 28 29 30 31 32 33 3435 #36 37 38 #39 40 41 42 43 44 45 46 47 #48 49
m st a

A. lacustris x A. schubarti

Y [ss ONA
fi 18s rDNA
Wi Hetrochromatic blocks
ANRHE WI MULT Nia PLAT Nondang Te
3.4 5 6 7 8 1112-13 Hi i i i i 20 22 23 24 25 26 28 29 3334 35 36 it lt 41 42 43 (i Ss(DNA+ 18s rDNA
m * Inconsistences

Figure 5. Ideogram of hybrids strains.

A. lacustris
Nt LR Cn INDNNNNNOononnnng
1 2 3 4 5 6 7 8 9 10 41 12 13 14 15 16 17 18 19 20 21 22 23 24 25
sm st a
H. anisitsi
2 3 4 5 6 if 8 9 10 11 12 13.14 15 16 17 #18 #19 «20 621 22. 23 24 = 25
m sm st a
A. fasciatus
RORERE ONNNHaRKAAE NDT ODNON NON NOD
1 2 3 4 5 6 7 8 9 410 i 12 13 14 15 16 17 18 19 20 21 22 23 24
sm st a
A. schubarti

Hi 5s rDNA

Wi 18s rDNA
WB Hetrochromatic blocks
4 5 6 7 a 3 40 14 42 1344 45 1647 48

Wl 5s rDNA + Heterochromatic blocks
om st a Hf 5s rDNA + 18s rDNA

Figure 6. Ideogram of parental strains.