Zoosyst. Evol. 98 (2) 2022, 285-303 | DO! 10.3897/zse.98.83749 > PENSUFT. Gee BERLIN Two new species of the genus Floresorchestia (Crustacea, Amphipoda, Talitridae) from Amphawa Estuary, Samut Songkhram Province, Thailand Anotai Suklom!, Tosaphol Saetung Keetapithchayakul’, Azman Abdul Rahim*, Koraon Wongkamhaeng? 1 Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand 2 Marine Ecosystem Research Centre, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia https://zoobank. org/5B12CCFC-3474-4F BE-8D4D-CDB971D36085 Corresponding author: Koraon Wongkamhaeng (koraon@gmail.com) Academic editor: Luiz F. Andrade # Received 14 March 2022 Accepted 21 June 2022 Published 15 July 2022 Abstract Two new species of Floresorchestia (Crustacea: Amphipoda) are described from Amphawa Estuary, Samut Songkhram Province, Thailand. Floresorchestia amphawaensis sp. nov. is a riparian-hoppers living near agricultural and urban areas, being distin- guished by the following character states: left mandible lacinia mobilis 4-dentate; gnathopod 2 palm reaching about 34%; telson as broad as long, with four robust setae per lobe. Floresorchestia pongrati sp. nov. are described as riparian-hoppers living in the moist area near Mae Klong canal banks with a unique left mandible lacinia mobilis 5-dentate; gnathopod 2 palm reaching 30% and telson with seven robust setae per lobe. The status and the problem of diagnostic character states of the genus Floresorchestia are discussed. Key Words Floresorchestiinae, riparian-hoppers, Southeast Asia, Talitridae Introduction Talitridae Rafinesque, 1815 is the only amphipod group adapted to live in terrestrial habitats. They show a global distribution, covering Northern and Southern Hemispheres and present a high diversity with 116 genera and about 358 species (Lowry and Myers 2019). Representatives of this family are also well recorded within the Southeast Asia Region with a total of 24 species (Asiaorchestia Lowry & Myers, 2019: 1 species; Floresorchestia Bousfield, 1984: 9 species; Gazia Lowry & Springthorpe, 2019: 1 species; Morinaga Lowry & Myers, 2019: 1 species; Platorchestia Bousfield, 1982: 2 species; 7alorchestia Dana, 1852: 9 species; and Vietorchestia Dang & Le, 2011: 1 species) (Azman et al. 2022). The subfamily Floresorchestiinae Lowry & Springthorpe, 2019 was introduced to re-order the Floresorchestia-complex, with the division into three genera (Austropacifica, Floresorchestia and Gazia). The three genera share one indicative synapomorphic character in the presence of slits on the epimera (slits may vary on either epimera 1-3, 2 or 2—3). The genus Floresorchestia contains 24 valid species confined to the Indo-Pacific (Lowry and Springthorpe 2019) and East Afrotropical realms, comprising five ecological types (Lowry and Springthorpe 2019). Recent discoveries have also found three new terrestrial species, one being from China (F) xueli Tong & Hou in Tong, Hao, Liu, Li & Hou, 2021), one from Thailand (F’ kongsemae Suklom, Danaisawadi & Wongkamhaeng, 2021) and another one from Kenya (F. mkomani Bichang’a & Hou in Bichang’a, Kioko, Liu, Li & Hou, 2021). Although previous studies on terrestrial amphipods in Thailand report four species of Floreshorchestiinae, Copyright Suklom, A. et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 286 such as G. samroiyodensis Azman, Wongkamhaeng & Damrongrojwattana, 2014, F. boonyanusithii Wongkam- haeng, Damrongrojwattana & Pattaratumrong, 2016, FE. buraphana Wongkamhaeng, Damrongrojwattana & Pattaratumrong, 2016 and F) kongsemae (Azman et al. 2014; Wongkamhaeng et al. 2016; Suklom et al. 2021), this number is still low given the whole picture of talitrid diversity in Thailand. Here, we describe two new Flore- sorchestia species, F'. amphawaensis sp. nov. and F. pon- grati sp. nov. including comparative morphology with four Floresorchestiinae species in Thailand. In addition, an updated key to the male Floresorchestia in Southeast Asia is provided. Materials and methods This study is based upon material collected from leaf litter in a mangrove forest of Amphawa District, Samut Songkhram Province, Thailand (Fig. 1). Specimens were collected using a pit-fall trap and were then carefully trans- ferred into plastic containers. They were fixed in 70% eth- anol and preserved in 95% ethanol. The specimens were ifr i . _ Thailand Tha Chin River Mae Klong River Suklom, A. et al.: Two Floresorchestia from Thailand examined under a compound microscope and later select- ed for dissection. The dissected appendages of specimens were examined and representative figures were produced using camera lucida attached to an Olympus CH30 light microscope. The pencil drawings were scanned and dig- itally inked using an iPad via the Procreate application. Final plates were prepared using Adobe Photoshop CC 2017. Distributions maps were plotted using SimpleMap- pr (Shorthouse 2010). The palm measurement length was made following Lowry and Springthorpe (2015) as a per- centage of the length of the propodus of male gnathopod 2. The percentage is calculated using the formula 100(1 - a/b)% (Fig. 2), where ‘a’ is the length of the posterior margin measured from the seta at the corner of the palm to the base of the propodus and ‘b’ is the length of the propodus measured from the base of the dactylus to the base of the propodus. Setae and mouthparts were made following Zimmer et al. (2009). Abbreviations for Figs 4-13: A, antenna; EP, epimera; G, gnathopod; LL, lower lip; MD, mandible; MX, maxilla; MP, maxilliped; P, pereopod; PL pleopod; TL, telson; U, uropod; UL, upper lip; R, right; L, left; 2, male; 9, female. Chao Phraya River Figure 1. Map showing the sampling area. Red circle represents type locality of Floresorchestia amphawaensis sp. nov. and blue circle represents type locality of F? pongrati sp. nov. zse.pensoft.net Zoosyst. Evol. 98 (2) 2022, 285-303 287 Figure 2. The measurement method for the length of male gnathopod 2 palm and posterior margin of propodus. Results Systematics Superfamily Talitroidea Bulycheva, 1957 Family Talitridae Rafinesque, 1815 Subfamily Floresorchestiinae Myers & Lowry, 2020 Floresorchestia Bousfield, 1984 Orchestia floresiana group Bousfield, 1971: 267. Floresorchestia Bousfield, 1984: 205——Miyamoto and Morino 2008: 838.—Lowry and Springthorpe 2009: 121. Type species. Orchestia floresiana Weber, 1892, original designation. Diagnostic description (modified from Lowry and Springthorpe 2015, 2019). Antenna | short, not longer than article 4 of antenna 2 peduncle. Antenna 2 pedun- cular articles slender; article 3 without ventral process. Mandible left lacinia mobilis 4—5-cuspidate. Maxilliped palp article 2 distomedial lobe well developed, article 4 reduced, button-shaped. Gnathopod 1 subchelate; poste- rior margin of merus, carpus and propodus each with lobe covered in palmate setae. Gnathopod 2 subchelate; dac- tylus distally attenuated (except Floresorchestia papeari Lowry & Springthorpe, 2015). Pereopods 3—7 cuspidac- tylate. Pereopods 6—7 not sexually dimorphic. Pleopods all well developed, biramous. Epimera 1-3, 2—3 or 2 with slits just above ventral margins, vestigial on EP1 (except F. xeuli Tong, Hao, Liu, Li & Hou, 2021). Uropods 1 and uropod 2 not sexually dimorphic. Uropod 1 outer ramus without marginal robust setae. Uropod 2 outer ramus with marginal robust setae. Uropod 3 ramus subequal in length to peduncle. Telson with 3—7 robust setae. Female (sexually dimorphic characters). Gnatho- pod 1 posterior margin of merus, carpus and propodus each without lobe covered in palmate setae. Gnathopod 2 mitten-shaped. Oostegites on gnathopod 2 to pereopod 5, setae straight. Species composition. Floresorchestia contains 27 species + 2 new species: Ff. amphawaensis sp. nov.; F. andrevo Lowry & Springthorpe, 2015; F. anomala (Chevreux, 1901); FF. anoquesana (Bousfield, 1971); F. anpingensis Miyamoto & Morino, 2008; F. boonyanusithii Wongkamhaeng, Dumrongrojwattana & Pattaratumrong, 2016; F. buraphana Wongkamhaeng, Dumrongrojwattana & Pattaratumrong, 2016; F. floresiana zse.pensoft.net 288 (Weber, 1892); F. hanoiensis Hou & Li, 2003; F. kalili Lowry & Springthorpe, 2015; F. kongsemae Suklom, Danaisawadi & Wongkamhaeng, 2021; F’. /aurenae Lowry & Springthorpe, 2015; F. malayensis (Tattersall, 1922); F. mkomani Bichang’a & Hou in Bichang’a, Kioko, Liu, Li & Hou, 2021; F. oluanpi Lowry & Springthorpe, 2015; F. palau Lowry & Myers, 2013; F. papeari; F: pectenispina (Bousfield, 1970); F. pohnpei Lowry & Myers, 2013; F. poorei Lowry & Springthorpe, 2009; F.. pongrati sp. nov.; Ff. samoana (Bousfield, 1971); F’ serejoae Lowry & Springthorpe, 2015; F.. seringat Lowry & Springthorpe, 2015; F. thienemanni (Schellenberg, 1931); F vitilevana (J.L. Barnard, 1960); F. xueli Tong, Hao, Liu, Li & Hou, 2021; F. yap Lowry & Springthorpe, 2015; F. yehyuensis Miyamoto & Morino, 2008. Floresorchestia amphawaensis sp. nov. https://zoobank.org/37893B87-E408-4CDE-A 8FA-B1CA22047C1F Type material. Holotype. ¢, 7.8 mm, THNHM-lv-18763, THAILAND, Amphawa District, Samut Songkhram, Thailand (13°26'27.8"N, 99°57'41.3"E), 21 February 2021, Piyangkun Lueangjaroenkit; THNHM-lv-18764. Allotypes, 2 collected with holotype; THNHM- lv-18764; Paratypes 5 3 and 10 & collected with holo- type; THNHM-lv-18765. Type locality. man-made ditch in coconut planta- tion, Amphawa District, Samut Songkhram, Thailand (13°26'27.8"N, 99°57'41.3"E). Habitat. Riparian-hoppers living near agricultural and urban areas in Amphawa District. Etymology. Named for the District of Samut Songkh- ram, Thailand where the species occurs. Ecological type. Riparian-hoppers (edges of lakes un- der stones or in very wet vegetation, near (or in) streams, rivers, creeks, cascades and waterfalls). Description. Based on holotype, male, 7.8 mm, THNHM-lv-18763. Head (Fig. 3FA) Eye large (greater than 1/2 the length of the head). Antenna I (Fig. 4A1) short, rarely longer than article 4 of antenna 2 peduncle. Antenna 2 (Fig. 4A2) shorter than half body length; peduncular article slender; article 3 shortest; article 5 longer than article 4; flagellum with 13 articles, final article cone-shaped with an apical cluster of setae. Upper lip (Fig. 5UL) broad, deep, apex rounded, with- out robust setae. Lower lip (Fig. 5LL) present; without inner plates. Left mandible (Fig. SLMD) incisor 6-den- tate; lacinia mobilis 4-dentate and four plumose setae in one row; molar strong with 16 striations and one plumose seta. Right mandible (Fig. 5RMD) incisor 5-dentate; lac- inia mobilis with numerous cusps, group of three robust setae and cluster of fine setae; molar strong with 18 stria- tions and one plumose seta. Maxilla I (Fig. 5MX1) inner plate slender with two terminal plumose setae; outer plate with seven robust serrated setae; with small 1-articulate palp. Maxilla 2 (Fig. 5MX2) plates narrow, inner plate zse.pensoft.net Suklom, A. et al.: Two Floresorchestia from Thailand slightly shorter than outer; with 14 subapical robust se- tae, one plumose seta and 9 facial setae; outer plate with 18 apical robust setae in two rows and five simple setae in outer corner. Maxilliped (Fig. SMP) inner plate apical and subapical margins with plumose setae, robust setae and two large conical robust setae; outer plate with two rows subapical setae and plumose setae; palp article 2 di- stomedial lobe well developed with numerous setae; arti- cle 4 reduced, button-shaped. Pereon. (Figs 4, 6) Gnathopod I (Fig. 4G1) sexually dimorphic; subchelate, coxa smaller than coxa 2, ven- tral margin lined with five robust setae, anterior margin straight; basis expanded posteriorly, anterior margin with four setae, posterodistal margin with two setae; posterior of merus, carpus and propodus each with lobe covered in palmate setae; carpus 1.5x longer than propodus, anterior margin with two robust setae, posterior margin with four robust setae; propodus subtriangular with well-developed posterior lobe, anterior side with three groups of coupled robust setae, posterior margin with five robust setae, palm slightly serrated, lined with nine robust setae; dactylus cuspidactylate, shorter than palm. Gnathopod 2 (Fig. 4G2) sexually dimorphic; sub- chelate; coxa anterior margin with two setae and ventral margin with seven setae; basis anterior margin smooth, slightly expanded, posterior margin with three robust setae; ischium with rounded lobe on anterior margin; posterior of merus, carpus and propodus glabrous; car- pus triangular, reduced, enclosed by merus and propodus, posterior lobe absent; propodus subovate, 1.4x as long as wide, palm reaching about 34%, posteromedial surface of propodus with groove, anterior side naked, palm lined with 11 robust setae; dactylus longer than palm fitted with facial groove, attenuated distally. Pereopod 3 (Fig. 6P3) coxa longer than broad with posterior process, anterior margin with seven robust se- tae; basis slightly expanded, anterior margin with three robust setae, posterior margin with three robust setae; is- chium shortest; merus longer than ischium, anterior and posterior margin of merus lined with clusters of robust se- tae; carpus and propodus slightly expanded; carpus poste- rior margin with cluster of robust setae; dactylus without notch on posterior margin. Pereopod 4 (Fig. 6P4) slightly shorter than pereopod 3; coxa longer than broad, anterior margin with five ro- bust setae and with small posterior process; basis longest, posterior margin with three robust setae; ischium short- est, posterodistal with two robust setae; merus and carpus shorter than those of pereopod 3; merus distally expand- ed, longer than carpus and propodus, anterior and pos- terior margin with five robust setae; carpus shorter than propodus posterior margin with three clusters of robust setae; propodus slender, with three clusters of robust se- tae in posterior side; dactylus slender and longer than that of pereopod 3, thickened proximally with a notch along posterior margin. Pereopod 5 (Fig. 6P5) coxa bilobed, anterior lobe dis- tinctly large than posterior lobe; basis anterior margin Zoosyst. Evol. 98 (2) 2022, 285-303 289 Figure 3. Male habitus of Floresorchestia spp. FA. F. amphawaensis sp. nov. holotype, male, 7.8 mm (THNHM-lv-18763), Ampha- wa District, Samut Songkhram, Thailand; FP. F’ pongrati sp. nov. holotype, male, 10 mm (THNHM-lv-19369), Amphawa District, Samut Songkhram, Thailand. Scale bars: 1 mm (FA, FP). with five robust setae, posterior margin with row of tiny setae; merus and carpus distally expanded; carpus short- er than propodus; propodus slender, longer than merus and carpus. Pereopod 6 (Fig. 6P6) coxa bilobed, posterior lobe larger than anterior; basis expanded, anterior margin with 10 robust setae, posterior margin with row of tiny setae; ischium shortest; merus distally expanded, anterior side with four clusters of robust setae; carpus expanded less than merus, anterior margin lined with four clusters of robust setae; propodus slender, longer than merus and carpus; dactylus slender with subapical robust setae. Pereopod 7 (Fig. 6P7) coxa reduced; posterior margin of basis with distinct minute serration (each serrate with small seta), anterior margin with 10 robust setae; mer- us and carpus slightly expanded: propodus longer than each merus and carpus; dactylus slender with subapical robust setae. zse.pensoft.net 290 Suklom, A. et al.: Two Floresorchestia from Thailand Figure 4. Floresorchestia amphawaensis sp. nov. holotype. Scale bars: 0.25 mm (A1, A2, G2); 0.5 mm (G1). Pleon. (Fig. 7) Pleopods all well developed. Pleopod 1 (Fig. 7PL1) peduncle without marginal setae; biramous, outer ramus subequal in length to peduncle; inner ramus with 11 articles; outer ramus with seven articles. Pleopod 2 (Fig. 7PL2) peduncle without marginal setae; biramous, inner ramus subequal to outer ramus, shorter than pedun- cle; inner ramus with seven articles; outer ramus with six articles. Pleopod 3 (Fig. 7PL3) peduncle without margin- al setae; biramous, inner ramus subequal to outer ramus, zse.pensoft.net shorter than peduncle; inner ramus with nine articles; out- er ramus more than six articles. Epimera (Fig. 7EP), Epimeron I posterodistal corner slightly protruding, without slit. Epimera 2 and 3 each with slits above ventral margin, with 21 and 10 slits, re- spectively. Epimeron 3 posteroventral corner and ventral margin smooth. Uropod I (Fig. 7U1) peduncle with four robust se- tae in one row; inner ramus subequal in length to outer Zoosyst. Evol. 98 (2) 2022, 285-303 MxX2 \ 291 Figure 5. Floresorchestia amphawaensis sp. nov. holotype. Scale bars: 0.1 mm (LL, LMD, MP, MX1-—2, RMD, UL). ramus, inner ramus with five marginal robust setae, api- cal with three robust setae; outer ramus without marginal robust setae, apical with three robust setae. Uropod 2 (Fig. 7U2) peduncle with three robust setae; inner ra- mus subequal in length to outer ramus; inner ramus with two marginal robust setae, apical with four robust se- tae; outer ramus with one marginal robust seta, apical with two robust setae. Uropod 3 (Fig. 7U3) uniramous, peduncle with four robust setae; ramus subequal to pe- duncle, without marginal robust setae and three apical robust setae. Telson (Fig. 7TL) as long as broad, weakly cleft, dor- sal mid-line at least halfway, with one marginal, three apical robust setae and two plumose setae. Female (Sexually dimorphic characters) (Fig. 8). Type. Allotype, female, 8.9 mm, THNHM-lv-18764. Pereon. Gnathopod I (Fig. 8G1) merus lacking tu- mescent lobe, posterior margin with three robust setae; propodus without tumescent protuberance, anterior mar- gin with three clusters of robust setae, posterior margin with six robust setae; palm slightly transverse, dactylus inner lateral posterior margin with four robust setae. zse.pensoft.net 292 Suklom, A. et al.: Two Floresorchestia from Thailand Figure 6. Floresorchestia amphawaensis sp. nov. holotype. Scale bars: 0.25 mm (P3—P7). Gnathopod 2 (Fig. 8G2) mitten-shaped; posterior margin of carpus and propodus each with lobe covered in palmate setae; carpus well developed, posterior lobe projecting between merus and propodus; nearly twice as long as wide; palm obtuse, lined with 10 robust setae, posterodistal corner with two robust setae; dactylus not modified distally, blunt. Remarks. Floresorchestia amphawaensis sp. nov. is similar to F! malayensis with which they share characteristics such as: (1) left mandible lacinia mobilis with 4-dentate; (2) gnathopod 1 carpus longer by 1.5x than propodus; (3) gnathopod 2 dactylus posterior margin smooth; (4) gnathopod 2 propodus 1.4* as long as wide; (5) uropod 1 peduncle with 4-6 robust setae; (6) uropod 3 ramus without marginal setae; (7) telson with four robust setae per lobe; (8) epimera 2—3 with slits. zse.pensoft.net However, F? amphawaensis sp. nov. may be distinguished from F; malayensis by the following [Characters of F: malayensis in brackets]: (1) gnathopod 2 palm reaching 34% [palm reaching 30%]; (2) uropod | inner ramus with five marginal setae [four marginal setae]; (3) uropod 1 peduncle with four robust setae [with five robust setae]; (4) uropod 3 peduncle with four robust setae [two robust setae]; (5) telson slightly longer than broad [broader than long]. Floresorchestia pongrati sp. nov. https://zoobank.org/FF30E55E-21A0-419A-AB32-D8A6C027654F Type material. Holotype. 3, 10 mm, THNHM-ly-19369, THAILAND, Amphawa District, Samut Songkhram, Thailand (13°26'03.8"N, 100°00'36.2"E), 10 April Zoosyst. Evol. 98 (2) 2022, 285-303 293 Figure 7. Floresorchestia amphawaensis sp. nov. holotype. Scale bars: 0.1 mm (PL1—PL3, U1, U2); 0.2 mm (EP, TL, U3). 2021, Piyangkun Lueangjaroenkit; THNHM-lv-19370, Paratype, 1 3 and 5 & collected with holotype. Type locality. Moist area near Mae Klong Canal banks, (13°26'03.8"N, 100°00'36.2"E), Muang Samut Songkhram District, Samut Songkhram, Thailand. Habitat. Riparian-hoppers living in the moist area near Mae Klong canal banks of Amphawa District, Samut Songkhram, Thailand. Etymology. The species is named in honour of Assis- tant Professor Pongrat Dumrongrojwattana from Burapha University, Thailand for his contribution on terrestrial Floresorchestiinae diversity in Thailand. Ecological type. Riparian-hoppers (edges of lakes un- der stones or in very wet vegetation, near (or in) streams, rivers, creeks, cascades and waterfalls). Description. Based on holotype, male, 10 mm, THNHM-lv-19369. Head. (Fig. 3FP) Eye medium (1/3 the length of the head). Antenna I (Fig. 9A1) short, rarely longer than article 4 of antenna 2 peduncle. Antenna 2 (Fig. 9A2) shorter than half body length; articles slender, article 5 longer than article 4; flagellum with 18 articles, final arti- cle cone-shaped with an apical cluster of setae. Upper lip (Fig. 10UL) broad, deep, apex rounded, without robust setae. Lower lip (Fig. 1OLL) present; with- out inner plates. Left Mandible (Fig. 1OLMD) incisor 6-dentate; left lacinia mobilis 5-dentate and four plumose setae in one row; molar strong with 28 striations and one plumose seta. Right mandible (Fig. 1ORMD) incisor 5-dentate; lacinia mobilis with numerous dents, three ro- bust setae and cluster of fine setae; molar strong with 22 striations and one plumose seta. Maxilla I (Fig. 1|OMX1) inner plate slender with two terminals plumose setae; the outer plate with eight robust serrated setae, with small l-articulate palp. Maxilla 2 (Fig. 1OMX2) plates narrow, inner plate slightly shorter than outer; inner plate with 16 subapical robust setae, one plumose seta and seven facial setae; outer plate with 26 apical robust setae in two rows. Maxilliped (Fig. 1OMP) inner plate apical and subapical with plumose setae, robust setae and two large conical robust setae; outer plate with two rows subapical setae and plumose setae; palp article 2 distomedial lobe well developed with numerous setae; article 4 reduced, but- ton-shaped. Pereon. Gnathopod I (Fig. 9G1) sexually dimorphic; sub-chelate, coxa smaller than coxa 2, ventral margin lined with five robust setae, anterior margin straight; basis slightly expanded, anterior margin with five setae, pos- terodistal margin with two setae; posterior of merus, car- pus and propodus each with lobe covered in palmate se- tae; carpus longer by 1.2x than propodus, anterior margin with one robust seta and three distal robust setae, posterior zse.pensoft.net 294 Suklom, A. et al.: Two Floresorchestia from Thailand Figure 8. Floresorchestia amphawaensis sp. nov. allotype, female 8.9 mm (THNHM-Iv-18764) Amphawa District, Samut Songkh- ram, Thailand. Scale bars: 0.25 mm (G1, G2, TL); 1 mm for habitus. margin with three robust setae; propodus subtriangular with well-developed posterior lobe, anterior side with five robust setae, posterior lobe with five robust setae, palm slightly serrated, lined with six robust setae; dactylus cus- pidactylate, shorter than palm, with two robust setae. zse.pensoft.net Gnathopod 2 (Fig. 9G1) sexually dimorphic; subche- late; coxa anterior margin with 13 robust setae; basis an- terior margin smooth, slightly expanded, posterior mar- gin with four robust setae; ischium with rounded lobe on anterior margin; posterior of merus, carpus and propodus 295 Zoosyst. Evol. 98 (2) 2022, 285-303 Figure 9. Floresorchestia pongrati sp. nov. holotype. Scale bars: 0.25 mm (A1, A2, G2); 0.5 mm (G1). without lobe covered in palmate setae; carpus triangular, reduced, enclosed by merus and propodus, posterior lobe absent; propodus subovate, 1.4< as long as wide, palm reaching about 30%, posteromedial surface of propodus with groove, anterior side naked, palm lined with 19 robust setae; dactylus longer than palm fitted with facial groove, attenuated distally, inner margin lined with 11 robust setae. Pereopod 3 (Fig. 11P3) coxa longer than broad with pos- terior process, anterior margin with nine robust setae; basis slightly expanded, anterior margin with four robust setae and posterior margin with four robust setae, with two posterodis- tal robust setae; ischium shortest; anterior and posterior mar- gin of merus lined with four clusters of robust setae; carpus and propodus slightly expanded; carpus posterior margin with three cluster of robust setae; propodus slender and lon- ger than carpus; dactylus without notch on posterior margin. Pereopod 4 (Fig. 11P4) slightly shorter than pereo- pod 3; coxa longer than broad, anterior margin with zse.pensoft.net 296 If) [tf | \/ fa | if / HIN K /| | idl \ NN, \ | HI \V ING) VW) /, i \ Suklom, A. et al.: Two Floresorchestia from Thailand Figure 10. Floresorchestia pongrati sp. nov. holotype. Scale bars: 0.1 mm (LL, LMD, MP, MX1, MX2, RMD, UL). six robust setae and with small posterior process; basis longest posterior margin with five robust setae; ischium shortest, posterodistal with three robust setae; merus and carpus shorter than those of pereopod 3; merus distally expanded, longer than carpus and propodus, anterior and posterior margin with three and four clusters of robust se- tae, respectively; carpus shorter than propodus, posterior margin with one cluster of robust setae; propodus slender, with four clusters of robust setae in posterior side; dacty- lus slender and longer than that of pereopod 3, thickened proximally with a notch mid-way along posterior margin. Pereopod 5 (Fig. 11P5) coxa bilobed, anterior lobe distinctly large than posterior lobe, posterior lobe with zse.pensoft.net five robust setae on ventral margin; basis anterior margin with four robust setae and two anterodistal robust setae, posterior margin with serrate and small setae; merus and carpus distally expanded; carpus shorter than propodus; propodus slender, longer than merus and carpus. Pereopod 6 (Fig. 11P6) coxa bilobed, posterior lobe larger than anterior; basis expanded, anterior margin with 10 robust setae, posterior margin with row of tiny setae; ischium shortest; merus distally expanded, anterior side with four clusters of robust setae; carpus expanded less than merus, anterior margin lined with five clusters of robust setae; propodus slender, longer than merus and carpus; dactylus slender without subapical robust setae. Zoosyst. Evol. 98 (2) 2022, 285-303 297 Figure 11. Floresorchestia pongrati sp. nov. holotype. Scale bars: 0.25 mm (P3—P7). Pereopod 7 (Fig. 11P7) coxa reduced; posterior mar- gin of basis with distinct minute serration (each serrate with small seta), anterior margin with 11 robust setae; ischium distally expanded; merus and carpus expanded; propodus longer than merus and carpus; dactylus slender with subapical robust setae. Pleon. (Fig. 12) Pleopods all well developed. Pleopod I (Fig. 12PL1) peduncle without marginal se- tae; biramous, outer ramus shorter than peduncle; inner ramus with 13 articles; outer ramus with 10 articles, first article lined with four plumose setae. Pleopod 2 (Fig. 12PL2) peduncle without marginal setae; bira- mous, outer ramus shorter than peduncle, inner ramus subequal to outer ramus, shorter than peduncle; inner ramus with 10 articles; outer ramus with 12 articles. Pleopod 3 (Fig. 12PL3) peduncle without marginal setae; biramous, inner ramus subequal to outer ramus, shorter than peduncle; inner ramus with 11 articles; out- er ramus 10 articles, first article lined with five plumose setae, shorter than peduncle. zse.pensoft.net 298 Suklom, A. et al.: Two Floresorchestia from Thailand Figure 12. Floresorchestia pongrati sp. nov. holotype. Scale bars: 0.1 mm (TL, U1—U3); 0.2 mm (PL1I-PL3, EP). Epimera (Fig. 12EP), epimeron I posterodistal cor- ner slightly protrudes, without slit. Epimera 2 and 3 each with slits above ventral margin with 27 and 29 slits, re- spectively. Epimeron 3 posteroventral corner and ventral margin smooth. Uropod I (Fig. 12U1) peduncle with six robust se- tae in one row; inner ramus subequal in length to outer ramus, inner ramus with marginal five robust setae in one row, apical with four robust setae; outer ramus with- out marginal robust setae, apical with four robust setae. Uropod 2 (Fig. 12U2) peduncle with five robust setae; inner ramus subequal in length to outer ramus; inner ra- mus with four marginal robust setae, apical with three robust setae; outer ramus with two marginal robust se- tae, apical with three robust setae. Uropod 3 (Fig. 12U3) uniramous, peduncle with one robust seta; ramus shorter than peduncle, with one marginal robust seta and three apical robust setae. Telson (Fig. 12TL) longer than broad, weakly cleft, dorsal mid-line at less than halfway, with four marginal and three apical robust setae. Female (Sexually dimorphic characters) (Fig. 13). Type. Paratype, female, 8.5 mm, THNHM-lv-19370. zse.pensoft.net Pereon. Gnathopod I (Fig. 13G1) merus lacking tu- mescent lobe, posterior margin with five robust setae; propodus without tumescent protuberance, anterior mar- gin with two robust setae, posterior margin with three clusters of robust setae; palm slightly transverse, dactylus inner lateral posterior margin with five robust setae. Gnathopod 2 (Fig. 13G2) mitten-shaped; posterior margin of carpus and propodus each with lobe covered in palmate setae; carpus well developed, posterior lobe projecting between merus and propodus; nearly twice as long as wide; palm obtuse, lined with 10 robust setae, posterodistal corner with two robust setae; dactylus not modified distally, blunt. Telson (Fig. 13TL) as broad as long, weakly cleft, dorsal mid-line less than halfway, with two marginal and three apical robust setae. Remarks. Floresorchestia pongrati sp. nov. 1s closely similar to F) papeari which has synapomorphic character states such as: (1) left mandible lacinia mobilis 5-dentate; (2) gnathopod 2 dactylus posterior margin smooth; (3) gna- thopod 1 carpus significantly longer (1.2) than propodus; (4) gnathopod 2 propodus (1.4) as long as wide; (5) uropod 3 ramus with marginal setae; (6) telson longer than broad Zoosyst. Evol. 98 (2) 2022, 285-303 299 Figure 13. Floresorchestia pongrati sp. nov. paratype, female 8.5 mm (THNHM-Iv-19370 Amphawa District, Samut Songkhram, Thailand. Scale bars: 0.1 mm (TL); 0.25 mm (G1, G2); 1 mm for habitus. zse.pensoft.net 300 @ Beach-hoppers ve Marsh-hoppers A Forest-hoppers v Field-hoppers @ Riparian-hoppers Suklom, A. et al.: Two Floresorchestia from Thailand OF seringat —~ Z malayensis a Figure 14. Distribution of the genus Floresorchestia spp. and (7) epimera 2—3 with slits. They are distinguished by the following [characters of F’ papeari in brackets]: 1) an- tenna 2 less than half body length [shorter than head and first three pereonites]; (2) gnathopod 2 dactylus posterior mar- gin smooth, proximal tooth absent [with proximal tooth]; (3) gnathopod 2 distally acute or attenuated [not modified distally, blunt]; (4) gnathopod 2 palm without protuberance near dactylar hinge [with rounded protuberance near dactyl- ar hinge]; (5) uropod 1 inner ramus with five marginal setae [four marginal setae]; (6) uropod 3 peduncle with one robust seta [five robust setae]; (7) telson with seven robust setae per lobe [five robust setae per lobe]. Discussion The genus Floresorchestia is one of the talitrid amphi- pods that can occupy both coastal and terrestrial habitats (Bousfield 1984; Lowry and Springthorpe 2015, 2019). Moreover, in terrestrial habitats, they also occupy a large range of habitats including marsh-hoppers, beach-hop- pers, field-hoppers, riparian-hoppers and forest-hoppers (Lowry and Springthorpe 2015). The beach-hoppers are the largest group with 13 species, followed by forest-hop- pers with five species and field-hoppers represented by three species. The present study increases the number of riparian-hoppers to four species. The wide variety of habitats implies the high adaptation ability which many studies presumed that the terrestrial species had a coast- al Floresorchestia ancestor (Bousfield 1984; Lowry and Springthorpe 2015, 2019). Floresorchestia amphawaensis sp. nov. and F. pongrati sp. nov. are sympatric. However, both species present different adaptations. F’ amphawaensis sp. nov. amphawaensis sp. nov. inhabits dry areas including the coconut plantation, man-made ditch and the man-made zse.pensoft.net pond in the Bang Prom temple, while F pongrati sp. nov. is limited to the river bank and attached to aquatic plants during high tide instead of migrating into drier places in the urban areas. Currently, a total of 15 species are distributed in the Southeast Asia region which presents more than 50% of known Floresorchestia in the world. As shown in Fig. 14, it is clear that coastal Floresorchestia species are more dominant than their terrestrial counterparts (and predominantly inhabit oceanic islands). Only F: kongsemae, F: boonyanusithii, F: malayensis and F: xue- /i were recorded in the mainland and some of them can establish their population in urban areas which are clas- sified as field- and forest-hoppers (Wongkamhaeng et al. 2016; Lowry and Springthorpe 2019; Suklom et al. 2021; Tong et al. 2021). Previous collecting efforts were substantially focused along the marine coastline while overlooking the inland areas that could certainly lead to low numbers of terrestrial species in the regions. Lowry and Springthorpe (2015) mentioned that each terrestrial species independently appeared on land and there is no phylogenetic link between the species. During our ob- servation, both F’ amphawaensis sp. nov. and F. pongrati sp. nov. occasionally attached to floating substrates, in- dicating their possible mode of dispersal. This strategy implies that further observation along the river basins might fit with the terrestrial species migratory pattern (Barnard 1971). At the beginning of this study, Azman et al. (2022) reported a total of three species (F boonyanusithii, FE. buraphana and F. kongsemae) from Thailand; two additional new ones (F. amphawaensis sp. nov. and FE. pongrati sp. nov.) are here described, bringing the number of known species from Thailand up to five species, respectively (Table 1). The Thai Floresorchestia spp. have character states such as: (1) gnathopod 1 palm angle Zoosyst. Evol. 98 (2) 2022, 285-303 Table 1. Summary of diagnostic characteristic of terrestrial amphipods in Thailand. 301 Species Antenna Left Male Male Uropod 1 Uropod 3 Slit on Telson 2 mandible| gnathopod 1 | gnathopod | gnathopod 2 | gnathopod epimera lacinia 1 carpus 2 propodus 2 and 3 mobilis Floresorchestia Less than | 4-dentate | Palmate lobe Carpus Palm Propodus | Inner ramus Peduncle 21 and | Telson as long amphawaensis half body on merus, slightly extending | 1.4x aslong| with four with four 10 slits as broad, sp. nov. length carpus and longer between 34 as wide marginal | robust setae, four robust propodus each | (1.2x) than | and 39% along robust setae | rami without setae and two covered in propodus posterior marginal setae plumose setae palmate setae margin per lobe Floresorchestia Shorter | 5-dentate | Palmate lobe Carpus Palm Propodus | Inner ramus | Peduncle with | 27 and | Telson longer pongrati sp. nov. than head on merus, slightly extending |1.4x aslong| with five one robust | 29 slits | than broad, and first carpus and longer between 30 as wide marginal | seta, rami with eight robust three propodus each | (1.2x) than | and 36% along robust setae} one marginal setae per lobe pereonite covered in propodus posterior seta palmate setae margin Floresorchestia Less than | 4-dentate | Palmate lobe Carpus Palm Propodus | Inner ramus Peduncle 27 and | Telson longer boonyanusithii half body on merus, weakly extending | 1.5-1.6x as | with four with two 20 slits | than broad, Wongkamhaeng, length carpus and longer between 31 | long as wide | marginal | robust setae, four robust Damrong rojwattana propodus each | (1.5x) than | and 35% along robust setae} rami without setae per lobe & Pattaratum rong, covered in propodus posterior marginal setae 2016 palmate setae margin Floresorchestia Less than | 5-dentate | Palmate lobe Carpus Palm Propodus | Inner ramus Peduncle 25 and | Telson longer buraphana half body on merus, slightly extending | 1.5-1.6x as | with three with two 15 slits | than broad, five Wongkamhaeng, length carpus and longer between 36 | long as wide | marginal | robust setae, robust setae Damrong rojwattana propodus each | (1.2x) than | and 40% along robust setae} rami without per lobe & Pattaratum rong, covered in propodus posterior marginal setae 2016 palmate setae margin Floresorchestia Shorter | 5-dentate | Palmate lobe Carpus |Palmreaching} Propodus | Inner ramus | Peduncle with | 23 and | Telson longer kongsemae Suklom, Danai sawadi & Wong kamhaeng, 2021 than head and first three pereonites on merus, carpus and propodus each covered in palmate setae subequal in length to propodus about 33% along posterior margin 1.5-1.6x as long as wide with three marginal robust setae three robust setae, rami with or without marginal setae 16 slits than broad, four robust setae per lobe Gazia samroiyodensis | Less than Male Posterior lobe Carpus Palm reaching Propodus Inner ramus Peduncle with 21 and Telson as long Azman, Dam half body | 4-dentate, | on carpus and slightly about 1.4x as long | with four two robust | 13 slits | as broad, five rongrojwattana & length female |propodus, each} — longer 32% along as wide marginal setae, rami robust setae Wongkamhaeng, 6-dentate | covered in (1.2x) than posterior robust setae} —_ with two per lobe 2014 palmate setae | propodus margin marginal setae transverse; (2) gnathopod 1 merus with well-developed palmate lobe; (3) gnathopod 2 palm reaching 30-40%; (4) gnathopod 2 dactylus posterior margin smooth, proximal tooth absent; (5) gnathopod 2 palm without protuberance near dactylar hinge; (6) gnathopod 2 distally attenuated; (7) pereopod 4 dactylus slightly to strongly thickened proximally, with notch mid-way along posterior margin and (8) epimera 2-3 with slits. On the other hand, they can be separated by nine combination characteristics (abbreviations in brackets represent species following; FE. amphawaensis sp. nov. [FAM], F boonyanusithii [FBO], F) buraphana [FBU], F’ kongsemae [FKO] and FE. pongrati sp. nov. [FPO]), consisting of: (1) antenna 2 less than half body length [FAM, FBO, FBU], shorter than head and first three pereonite [FKO, FPO]; (2) left mandible lacinia mobilis four dentate [FAM, FBO], five dentate [FBU, FKO, FPO]; (3) male gnathopod 1 carpus slightly longer (1.2) than propodus [FAM, FBU, FPO], weakly longer (1.5x) than propodus [FBO]; subequal in length to propodus [FKO]; (4) male gnathopod 2 propodus 1.4x as long as wide [FAM, FPO], 1.5—1.6* as long as wide [FBO, FBU, FKO]; (5) uropod 1 inner ramus with three marginal robust setae [FBU, FKO], with four marginal robust setae [FAM, FBO], with five marginal robust setae [FPO]; (6) uropod 3 peduncle with one robust seta [FPO], with two robust setae [FBO, FBU], with three robust setae [FKO], with four robust setae [FAM]; (7) uropod 3 rami without marginal setae [FAM, FBO, FBU, FKO], with marginal setae [FPO, FKO]; (8) telson as long as broad (FAM), longer than broad [FBO, FBU, FKO, FPO]; and (9) telson with four robust setae per lobe [FAM, FBO, FKO], with 5—7 robust setae per lobe [FBU, FPO]. There has been concern about the status of Floresorchestia xueli which was described from Yunnan, China (Tong et al. 2021) and the F) vitilevana (J.L. Banard 1960) from Nandaivatu and Mt. Victoria, Fiji (Lowry and Springthorpe 2015). The authors mentioned the distinct characteristics of the species on coxal gills complexly lobed and convoluted; and epimeral plates without slits. According to the diagnostic description of the subfamily Floresorchestiinae, all members including the genus Austropacifica, Floresorchestia and Gazia present maxilliped palp article 2 with distomedial lobe; article 4 reduced, button-shaped or fused to article 3 and epimera 1-3 slits present on at least one plate (Myers and Lowry 2020). Even other characteristics of F’ xueli fit with the diagnosis of the genus Floresorchestia, except for the epimera 1—3 without slit. For this reason, F’ xueli should be reclassified and assigned to another subfamily and genus. zse.pensoft.net 302 Suklom, A. et al.: Two Floresorchestia from Thailand Key to species of the Floresorchestia in Southeast Asia and neighbouring regions 1 Gnathopodilacarous subequallor lessethansely 7 autos GropOGUs..t.r2 se. 225 Mie ece desea shlfe das adc nbd alts sees xeddisalba sé SMlege des caesoadlsDah fe 2 - Ghathopod™ «carous. lonsercl. 7 x-thai Pho pOGusS.. .as.2.dzede caso mee tha mee ece eee A ate F, thienemanni (Schellenberg, 1931) 2(1) Gnathoped Ii carpus signiticanthyionger €12-15 x) than Pro pOdus sexs Gow. eg oan ck eee an sees ee ih weer 3 - Ghatopoc. iecakoussubed Wal Iter toro pOctSe re. 8 mts ke core hee ee ks be ey ce wey pro ee) oes en re eek ee eee Fld as 12 3(2) Ghathoped: l-carpus-sisniticantly longer (ieAx) TNaMaproPOGUS i... Tiksts sso cne acsangebel beaded damegnssastered bebdaedha opens checmmnebediog 4 - Gnathopod 1 carpus significantly longer (1.5) than PropOduS ..............ccccccece cece eeeeeeeeceeeeeeeeee cece eeseeeeeeeeaeeeseeeseeeeaees 7 4(3) Gnathopod 2 propodus (1.3-1.4x) as long as wide, Gnathopod 2 propodus 1.4~x as long as wide ...............::::::006 5 - Ghathopoce2: prepodUssGl Sl GX)PAS OMS AS WIS. ii chiee pac celleeetagiennanderinle reed eden qae'egeae attilae det toler Une aay ew tebbiev nna seddieonawan 7 5(4) Uropod 1 peduncle without robust Setae ...............ccccecseccsceceeseessecnescaeeceeseesecsareeeneeess F, malayensis (Tattersall, 1922) - MODOC L DECUNCle witht4—S FOBUST SOlaGt ater re et ese ope beckon Wehr. cer I9 roel our soiwe a Nie 2 Ohi cn steven 000) 8 oe bP og Vo reeteeS eer) 6 6(5) Ws Ac Koln als rae SINUS ieoh10( <1 011: Nee kek ne Seen ne a a ERA ee beck ee SP a REE aR F, pongrati sp. nov. - Pet aC tee TODS wit eat GlSr teal bes Sa: 2c cee nkty ee Bess eect Al ene Oe 5 Ra a abl ace ue ee caehl F, amphawaensis sp. nov. 7(A) Left lacinia mobilis 5-dentate; Uropod 1 peduncle without marginal setae; Uropod 3 ramus without marginal robust SOLAS neck canteen ttetiita dnatrcnan ser eaath Ae a dm mawene seat chins tieten nada eaavca ae oe ude senat hee aoe an F. yehyuensis Miyamoto & Morino, 2008 - Left lacinia mobilis 4-dentate; Uropod 1 peduncle bearing more than six robust setae; Uropod 3 ramus with one INEST VANa SOUL Bi psd ite Seca enal rp ating ndash F. buraphana Wongkamhaeng, Damrongrojwattana & Pattaratumrong, 2016 &(3) POT eee CMM S11 Se eben S22 abel sean TB aan Bed Saban Tyee Whats seh nhe Maes sat Meaney oe reas tMe ae La EMU nn SOT PEE MELE ee Se Ne es cee Te 8 ~ EDIRIST abl —SrPMtwSIt weltelle) ees Ci teusk teens cet even asedel ys sage PND URR soon ns bi,5 F. laurenae Lowry & Springthorpe, 2015 - EDL Sa SSW ORM SENDER iia condense cantina ue Content etc Mtcan eee. APOE, ARUBA es Dade See il F. xueli Tong & Hou, 2021 9(8) GiietIepOd=2 PrOPOCUS wh “aS HOMO ASW Ces ee he eww Pee ed Aes deh een Soe ee) Be eer Pod 8 Pe ce oR ad Re 9 - Ghathopod'2 propodus=l.5—1 6x -as lOne AS WIS facts ss Ahawetes saaed debideddhysonetachemaneeeceubonhdoumedsns SuaedAebbodd hd sagmkscdiemeiartet 10 10(9) Telson broader than long; Gnathopod palm without protuberance near dactylar hinge; Uropod 3 ramus without mar- SIEM SEC IOUISURSOLCC.,. Sanur eras fice deck wey eaat hyd Se ees OA eas. cue EE ns ee, Poe yy Pe F. hanoiensis Hou & Li, 2003 - Telson longer than broad; Gnathopod palm with rounded protuberance near dactylar hinge; Uropod 3 ramus with rahe Nal CO MUSH SETAC | Gat Ras. 8c niebetdans dnathcase tae sag ls Muse yah Added achat inst sedans acta tse ish teeieldend: F. floresiana (Weber, 1892) Ilo). rGhabnepod.paliim-feacniae 40—SOSpens wren ay. = clever Be, Pris Cried a) oe sro Bem conse te Sor PUN, Ceres 0) ote Peed c let tack oN 11 - Gnathopod palm reaching 30-40% .. F. boonyanusithii Wongkamhaeng, Damrongrojwattana & Pattaratumrong, 2016 12¢lhS..Uropede2-outer tamus withtwe-marginal setaGaa. ..2.22-..cec0bsceenneg eeeeeress F. anpingensis Miyamoto & Morino, 2008 - Wropocg:2-ouler ramus With two: Margimal STAC... iscietsssceetcictanntd edtedaidawnctess F. oluanpi Lowry & Springthorpe, 2015 13(2) Telson about as long as broad; Antenna 2 longer than head and first three Pereonites .............c cece cece eeeeeeeeeeeeeeeeeaeees Pa dcrahiath deste P Aoi Baas Pedra: MRT Sahat ede ia ht ese Tee ARCs EN aot cells ety F. seringat Lowry & Springthorpe, 2015 - Telson longer than broad; Antenna 2 shorter than head and first three pereonites................c.cseceecceeeceeseeceeeceeseeeoeeees Se ore one reteset ee errs a Ree aPC ee ee eee bore, I F. kongsemae Suklom, Danaisawadi & Wongkamhaeng, 2021 Acknowledgements References This work was financially supported by the Office of the Ministry of Higher Education, Science, Research and Innovation; and Thailand Science Research and Innovation through the Kasetsart University Reinventing University Program 2021. This research is funded by Kasetsart University through the Graduate School Fellowship Program, Kasetsart University (Bangkok, Thailand) and grant from Kasetsart University Research and Development Institute (KURDI), Kasetsart University, Bangkok, Thailand. We would like to express our sincere thanks to the house of Lueangjaroenkit for all their kind supported during field trips. Thanks also go to Mr. Prapanth Iamwiriyakul for his invaluable advice on the nomenclature of the new taxa. We are grateful to the Department of Zoology, Faculty of Science, Kasetsart University for the laboratory facilities. zse.pensoft.net Azman AR, Sivajothy K, Shafie BB, Ja’afar N, Wongkamhaeng Kk, Bussarawit S, Alip AE, Lee YL, Metillo EB, Won MEQ (2022) The amphipod (Crustacea: Peracarida) of the Southeast Asia and the neighbouring waters: an updated checklist with new records of en- demic species. Research Bulletin — Phuket Marine Biological Center 79(1): 42-84. Barnard JL (1960) Crustacea: Amphipoda (strand and terrestrial Talitri- dae). Insects of Micronesia 4: 13-30. Barnard JL (1971) Keys to the Hawaiian marine Gammaridea, 0—30 meters. Smithsonian Contributions to Zoology 58(58): 1-135. https://doi.org/10.5479/s1.00810282.58 Bichang’a JS, Kioko EN, Liu H, Li S, Hou Z (2021) Two species of Talitridae (Crustacea, Amphipoda) from Kenya. Zootaxa 4927(3): 331-358. https://doi.org/10.11646/zootaxa.4927.3.2 Bousfield EL (1970) Terrestrial and aquatic amphipod Crustacea from Rennell Island. The Natural History of Rennell Island. British Solo- mon Islands 6: 155-168. Zoosyst. Evol. 98 (2) 2022, 285-303 Bousfield EL (1971) Amphipoda of the Bismarck Archipelago and adjacent Indo-Pacific islands (Crustacea). Steenstrupia |: 255—293. Bousfield EL (1984) Recent advances in the Systematics and Biogeog- raphy of Landhoppers (Amphipoda: Talitridae) of the Indo-Pacific Region. Bishop Museum Special Publications 72: 169-205. Chevreux E (1901) Crustacés Amphipodes. Mission scientifique de M. Ch. Alluaud aux Iles Séchelles (Mars, Avril, Mai 1892). Mémoires de la Société Zoologique de France 14: 388-438. Hou ZE, Li S (2003) Terrestrial talitrid amphipods (Crustacea: Amphipoda) from China and Vietnam: studies on the collection of IZCAS. Journal of Natural History 37(20): 2441-2460. https://doi. org/10.1080/00222930210144343 Lowry JK, Myers AA (2013) New species of Floresorchestia from Micronesia living in unusual habitats (Crustacea, Amphipoda, Tal- itridae). Zootaxa 3737(5): 576-584. https://doi.org/10.11646/zoot- axa.3737.5.4 Lowry JK, Myers AA (2019) New genera of Talitridae in the revised Superfamily Talitroidea Bulycheva 1957 (Crustacea, Amphipoda, Senticaudata). Zootaxa 4553(1): 100. https://doi.org/10.11646/zoo- taxa.4553.1.1 Lowry JK, Springthorpe RT (2009) The genus Floresorchestia (Amphipoda: Talitridae), on Cocos (Keeling) and Christmas Islands. Memoirs of the Museum of Victoria 66(1): 117-127. https://doi. org/10.24199/). mmy.2009.66.13 Lowry JK, Springthorpe RT (2015) The tropical talitrid genus Floresorchestia (Crustacea, Amphipoda, Talitridae). 3935(1): 1-68. https://doi.org/10.11646/zootaxa.3935.1.1 Lowry JK, Springthorpe RT (2019) Talitrid amphipods from India, East Africa and the Red Sea (Amphipoda, Senticaudata, Talitroidea, Tal- itridae). Zootaxa 4638(3): 351-378. https://doi.org/10.11646/zoot- axa.4638.3.3 Miyamoto H, Morino H (2008) Taxonomic studies on the Talitridae Zootaxa (Amphipoda) From Taiwan, HI. The Genus Floresorchestia Bousfield, 1984. Crustaceana 81(7): 837-860. _ https://do1. org/10.1163/15685400878477 1667 Myers AA, Lowry JK (2020) A phylogeny and classification of the Talitroidea (Amphipoda, Senticaudata) based on interpretation of morphological synapomorphies and homoplasies. Zootaxa 4778(2): 281-310. https://doi.org/10.11646/zootaxa.4778.2.3 Rafinesque CS (1815) Analyse de la nature ou Tableau de I’ Universitet des corps organises. Palerme, 224 pp. https://doi.org/10.5962/bhl. title. 106607 Schellenberg A (1931) XVIII. Amphipoden der Sunda-Expeditionen Thienemann und Rensch. Sonder-Abdruck aus dem Archiv fiir Hy- drobiologie, Suppl.-Bd. I. (“Tropische Binnengewasser, Band I’), S. 493-511. Shorthouse DP (2010) SimpleMappr, an online tool to produce publi- cation-quality point maps. http://www.simplemappr.net [accessed 5 February 2022] Suklom A, Danaisawadi P, Wongkamhaeng K (2021) Floresorchestia kongsemae sp. n. a new species (Crustacea: Amphipoda: Talitridae) from Kasetsart University, Bangkok, Thailand. Biodiversity Data Journal 9: e63197. https://doi.org/10.3897/BDJ.9.e63197 Tattersall WM (1922) Zoological results of a tour in the Far East. Am- phipoda with notes on an additional species of Isopoda. Memoirs of the Asiatic Society of Bengal 6: 435-459. 303 Tong Y, Hao J, Liu H, Li S$, Hou Z (2021) Floresorchestia xueli, a new terrestrial crustacean (Amphipoda, Talitridae) from Yunnan, China. Zootaxa 499(2): 318-330. https://doi.org/10.11646/zootaxa.499 1.2.5 Weber M (1892) Der Susswasser-Crustaceen des Indischen Archipels, nebst bemerkungen uber die Susswasser-Fauna im Allgemeinen. Zoologisclze Ergebnisse einer Reise nach niederlaizdischen Ost-in- dien 2: 528-571. Wongkamhaeng K, Dumrongrojwattana P, Saengsakda Pattaratumrong M (2016) Two new species of Floresorchestia (Crustacea, Amphipoda, Talitridae) in Thailand. ZooKeys 635: 31—51. https:// doi.org/10.3897/zookeys.635.10454 Zimmer A, Araujo PB, Bond-Buckup G (2009) Diversity and ar- rangement of the cuticular structures of Hyalella (Crustacea: Amphipoda: Dogielinotidae) and their use in taxonomy. Zoo- logia (Curitiba) 26: 127-142. https://doi.org/10.1590/S1984- 46702009000100019 Supplementary material | Figure S1 Authors: Anotai Suklom, Tosaphol Saetung Keetapithchayakul, Azman Abdul Rahim, Koraon Wongkamhaeng Data type: TIF file Explanation note: Figure S1. Live Floresorchestia am- phawaensis sp. nov. in their natural habitat. Copyright notice: This dataset is made available under the Open Database License (http://opendatacommons. org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow us- ers to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited. Link: https://do1.org/10.3897/zse.98.83749 suppl 1 Supplementary material 2 Figure S2 Authors: Anotai Suklom, Tosaphol Saetung Keetapith- chayakul, Azman Abdul Rahim, Koraon Wongkam- haeng Data type: TIF file Explanation note: Figure S2. Live Floresorchestia pon- grati sp. nov. in their natural habitat. Copyright notice: This dataset is made available under the Open Database License (http://opendatacommons. org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow us- ers to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited. Link: https://do1.org/10.3897/zse.98.83749 suppl2 zse.pensoft.net