MycoKeys 94: | 7-35 (2022) er-reviewed open-access journal doi: 10.3897/mycokeys.94.9630 | < MycoKkeys https://mycokeys.pensoft. net Launched to accelerate biodiversity research Soil-borne Calonectria (Hypocreales, Nectriaceae) associated with Eucalyptus plantations in Colombia Nam Q. Pham', Seonju Marincowitz’, ShuaiFei Chen?*4, Carlos A. Rodas”°, Michael J. Wingfield! | Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa 2 Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa 3 Research Institute of Fast-growing Trees (RIFT), Chinese Academy of Forestry (CAF), Zhanjiang, Guangdong Province, China 4 China Eucalypt Research Centre (CERC), Chinese Academy of Forestry (CAF), Zhanjiang, Guangdong Province, China § Forestry Health Protection Programme, SmurfitKappa Colombia, Yumbo, Colombia Corresponding author: Nam Q. Pham (Nam.Pham@fabi.up.ac.za) Academiceditor:N. Wijayawardene| Received 16 October 2022 | Accepted 3 1 October2022 | Published30 November2022 Citation: Pham NQ, Marincowitz S, Chen SE, Rodas CA, Wingfield MJ (2022) Soil-borne Calonectria (Hypocreales, Nectriaceae) associated with Eucalyptus plantations in Colombia. MycoKeys 94: 17-35. https://doi.org/10.3897/ mycokeys.94.96301 Abstract Eucalyptus spp. are widely planted in Colombia as an important component of a growing paper and pulp industry. Leaf and shoot blight caused by Calonectria spp. was one of the first disease problems to emerge in these plantations. A survey of Eucalyptus plantations in four forestry regions of Colombia during 2016 resulted in a large number of Calonectria isolates from soil samples collected in the understories of trees having symptoms of Calonectria leaf and shoot blight. The aim of this study was to identify and resolve the phylogenetic relationships for these isolates using DNA sequence comparisons of six gene regions as well as morphological characters. From a collection of 107 isolates, seven Calonectria species residing in three species complexes were identified. Two of these represented undescribed species, namely C. exiguispora sp. nov. and C. guahibo sp. nov. Calonectria parvispora and C. spathulata were the most commonly iso- lated species, each of which accounted for approximately 30% of the isolates. The results suggest that Colombia has a wide diversity of Calonectria spp. and that these could challenge Eucalyptus plantation forestry in the future. Copyright Nam Q. Pham et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 18 Nam Q. Pham et al. / MycoKeys 94: 17-35 (2022) Keywords Calonectria leaf and shoot blight, Cylindrocladium, multi-gene phylogeny, taxonomy, two new taxa Introduction Colombian plantation forestry is based primarily on non-native Pinus and Eucalyptus species, which have been widely deployed as an important component of the growing wood and paper industry. These plantations are based on short rotations, and in the case of Eucalyptus, clonal propagation has been established rapidly during the course of the last decade. There are currently approximately 540 000 ha of commercially man- aged plantations, of which Eucalyptus makes up a substantial component (20%) of this resource (MADR; https://www.minagricultura.gov.co/). As plantation forestry has grown globally, damage due to insect pests and microbial pathogens has become increasingly important (Wingfield et al. 2008, 2015; Paine et al. 2011). Relevant diseases of planted Eucalyptus in Colombia include stem canker caused by species of Cryphonectriaceae and Botryosphaeriaceae (van der Merwe et al. 2001; Rodas et al. 2009), wilt and dieback caused by Ceratocystis neglecta (Rodas et al. 2008), Myrtle rust caused by Austropuccinia psidii (Rodas et al. 2015; Granados et al. 2017), as well as leaf and shoot blight caused by Calonectria species (Rodas et al. 2005). Of these, Calonectria leaf and shoot blight was amongst the first disease problems to emerge (Rodas et al. 2005; Rodas and Wingfield 2020). Species of Calonectria (Hypocreales, Nectriaceae) have a wide distribution globally, especially in tropical and sub-tropical regions (Crous 2002; Lombard et al. 2010b; Marin-Felix et al. 2017). These fungi represent some of the most aggressive pathogens of agricultural, forestry, horticultural and ornamental plants (Crous 2002; Lombard et al. 2010b). Calonectria spp. are best known as root, shoot and foliar pathogens and can be associated with various disease symptoms, including damping-off, seedling blight, leaf and shoot blight, leaf spot, stem lesions, collar and root rot, fruit rot, and cutting rot (Sharma et al. 1984; Mohanan and Sharma 1985; Crous et al. 1991, 1998; Ferreira et al. 1995; Crous 2002; Old et al. 2003; Lombard et al. 2010b; Lopes et al. 2018). In Colombia, the first outbreak of Calonectria leaf and shoot blight in Eucalyptus plantations occurred in 1998, where Calonectria spathulata was shown to be the pre- dominant pathogen (Rodas et al. 2005). High humidity and abundant free moisture in this region result in conditions highly conducive to disease outbreaks (Crous 2002; Rodas et al. 2005). Infections by Calonectria spp. have consequently resulted in severe defoliation and significant negative impacts on the growth of susceptible genotypes (Rodas et al. 2005). Calonectria spp. are typically soil-borne fungi and many of these move between the soil environment and the leaf canopy of host trees (Crous 2002; Li et al. 2022). Previ- ous studies of Calonectria leaf and shoot blight on Eucalyptus in Colombia considered only isolates from infected leaves (Rodas et al. 2005; Rodas and Wingfield 2020). In Soil-borne Calonectria spp. from Colombia 19 order to provide a more comprehensive overview of Calonectria species associated with Eucalyptus in Colombia, soil samples were collected from Eucalyptus plantations in Colombia, resulting in a large number of isolates. The aim of this study was to identify and resolve the phylogenetic relationships for these isolates using multi-gene DNA sequence comparisons as well as morphological characteristics. Materials and methods Sampling and fungal isolations During 2016, surveys of Eucalyptus plantations were conducted in different for- estry farms located across four provinces of Colombia, namely, Cauca, Risaralda, Valle del Cauca, and Vichada (Fig. 1; Suppl. material 2). Soil samples were taken in the understories of Eucalyptus trees having symptoms of Calonectria leaf and shoot blight. In addition, random soil samples were collected from the native vegetation surrounding the Eucalyptus plantations in these regions. Soils were packed in plastic bags and transferred to the laboratory for isolation. The samples were baited with germinating alfalfa (Medicago sativa) seeds following the method recommended by Crous (2002). A dissection microscope was used to locate conidiophores and conidia typical of Calonectria on the infected alfalfa sprouts. These were lifted from the infected tissues using a sterile hypodermic needle and transferred to Petri dishes containing 2% (w/v) malt extract agar (MEA; 20 g malt extract, Biolab, Midrand, South Africa; 20 g Difco agar, Becton Dickinson, Maryland, USA; 1 L deionised water). Primary isolations were incubated for 3—7 d at 25 °C to allow fungal growth. Single hyphal tips were cut from the fungal colonies, transferred to fresh MEA plates, and incubated at 25 °C to ob- tain pure cultures. These cultures were deposited in the culture collection (CMW) of the Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, South Africa. Representative cultures, including the ex-type strains of novel taxa, were deposited in the CMW-IA (the culture collection of Innovation Africa, University of Pretoria, Pretoria, South Africa). Dried-down specimens of sporulating cultures were deposited in the PRU (H.G.W.J. Schweickerdt Herbarium of the University of Preto- ria, Pretoria, South Africa). DNA extraction, PCR amplification and sequencing Prepman Ultra Sample Preparation Reagent (Thermo Fisher Scientific, Waltham, MA, USA) was used to extract the total genomic DNA from 7-d-old isolates grown on 2% MEA, following the manufacturer's suggested protocols. A fragment of the ac- tin (ACT), calmodulin (CIVDA), histone H3 (47/53), translation elongation factor l-alpha (TEF/), 8-tubulin (7UB2), and DNA-directed RNA polymerase II second largest subunit (RPB2) gene regions were amplified using the primers ACT-512F and 20 Nam Q. Pham et al. / MycoKeys 94: 17-35 (2022) ACT-783R (Carbone and Kohn 1999), CAL-228F and CAL-2Rd (Carbone and Kohn 1999; Groenewald et al. 2013) CYLH3F and CYLH3R (Crous et al. 2004), EF1- 728F and EF2 (O’Donnell and Cigelnik 1997; Carbone and Kohn 1999), T1 and CYLTUBI1R (O’Donnell and Cigelnik 1997; Crous et al. 2004), and fRPB2-5F and fRBP2-7cR (Liu et al. 1999), respectively. The PCR reactions and conditions were the same as those used by Pham et al. (2019) and Liu et al. (2020). ExoSAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA) was used to purify the Amplicons. Cleaned-up amplified fragments were sequenced in both directions using an ABI PRISM 3100 DNA sequencer (Thermo Fisher Scientific, Waltham, MA, USA) at the Sequencing Facility of the Faculty of Natural and Agricultural Sciences, University of Pretoria. Geneious Prime 2022.1.1 was used to assemble and edit the raw sequences (https:// www.geneious.com). Sequences obtained in this study were deposited in GenBank (hetp://www.ncbi.nlm.nih.gov). Phylogenetic analyses The sequences generated in this study were compared with those for previously published species of Calonectria sourced from the GenBank database (http://www. ncbi.nlm.nih.gov/) and subjected to phylogenetic analyses. Alignments of all se- quences were assembled using the online version of MAFFT v. 7 (http://mafft.cbre. jp/alignment/server/) (Katoh and Standley 2013) and then confirmed manually in MEGA v. 7 (Kumar et al. 2016). Maximum likelihood (ML) and Bayesian inference (BI) analyses were performed on data sets for each individual gene region and the combined data set. The most appropriate models were obtained using the software jModeltest v. 1.2.5. (Posada 2008). ML analyses were conducted using RaxML v. 8.2.4 on the CIPRES Science Gateway v. 3.3 (Stamatakis 2014) with a default GTR substitution matrix and 1,000 rapid bootstraps. BI analyses were performed using MrBayes v. 3.2.6 (Ronquist et al. 2012) on the CIPRES Science Gateway v. 3.3. Four Markov Chain Monte Carlo (MCMC) chains were run from a random start- ing tree for five million generations, and trees were sampled every 100" generation. The first 25% of trees sampled were eliminated as burn-in, and the remaining trees were used to determine the posterior probabilities. Sequences for two isolates (CBS 109167 and CBS 109168) of Curvicladiella cignea were used as the outgroup taxa in all phylogenetic analyses. Phylogenetic trees were viewed using MEGA v. 7 (Kumar et al. 2016). Morphology The isolates were grown on synthetic nutrient-poor agar (SNA) (Nirenberg 1981) or together with alfalfa sprouts to induce the production of the asexual structures. Fruit- ing structures were initially mounted in water and replaced with 85% lactic acid for observation. Crosses between single hyphal tip isolates on minimal salt agar (MSA) Soil-borne Calonectria spp. from Colombia 21 Guajira Atlantico | Risaralda < Sucre Bolivar ; S 1 i \ \ Cordoba . 4 Nortede’ | Vichada Santander 4 Valle del Cauca Antioquia Santander Arauca fr 4 " ie Choco : 5 Boyaca Caldas Casanare Risarak'a Cundinamarca i i Vichada Quindio Tolima P Wales Guania Guaviare Caqueta . Putumayo Amazonas @ Calonectria parvispora & Calonectria brachiatica e Calonectria pini & Calonectria colombiana & Calonectria spathulata & Calonectria exiguispora sp. nov. Scale: 1:3,000,000 & Calonectria guahibo sp. nov. 70 380 70 140 210 280 Km Figure |. Geographic location of the sampling sites in Colombia, indicated as blue dots on the map, and the diversity of Calonectria spp. isolated from each region. were made to induce the production of a sexual state, as described by Pham et al. (2019). Nikon microscopes (Eclipse Ni, SMZ 18, Tokyo, Japan) were used to study the morphological characteristics. Images were captured using a Nikon DS-Ri2 camera mounted on the microscopes using the NIS-Elements BR program. Up to fifty meas- urements were made of all characteristic structures whenever possible. Dimensions were presented as minimum-maximum and with average = standard deviation for the key morphological characteristics. 22 Nam Q. Pham et al. / MycoKeys 94: 17-35 (2022) Colony characteristics were observed on 6-d and 30-d-old cultures on 2% MEA. Colours were described using the charts of Rayner (1970). Three replicates for each species were prepared to determine the optimum growth temperature. A mycelial plug (5 mm diam) from the margins of actively growing 4 d-old cultures was transferred to the centres of Petri dishes containing MEA. These cultures were grown at temperatures ranging from 5—35 °C at 5 °C intervals. Colony diameters perpendicular to each other were measured when colony growth reached the edges of Petri dishes at an optimum temperature, and averages were computed. Results Fungal isolates A total of 107 isolates having morphological characteristics typical of Calonectria spp. were obtained from the soil samples (Suppl. material 2). Of these, 46 were from Cauca, 38 from Risaralda, 14 from Valle del Cauca, and nine from Vichada. Up to four different Calonectria spp. were detected in each of these regions (Figs 1, 2). 35 3.7% 1.9% r C. spathulata A > een ' & C. brachiatica \ @ C. guahibo @ C pini e C. colombiana & C. exiguispora 30 &) RIsARALDA {I} vate DEL cauca & cauca VICHADA LL} NUMBER OF ISOLATES Y NS N SQ C. spathulata C.parvispora C. brachiatica C. guahibo C. pini C.colombiana_ C. exiguispora Figure 2. Relative occurrence of Calonectria species associated with Eucalyptus plantations in Colombia. Different species are represented by different colours. Isolates obtained from different regions are repre- sented by different patterns in the bar chart. Soil-borne Calonectria spp. from Colombia 23 Two of the most commonly isolated species each accounted for approximately 30% of the isolates (Fig. 2). The remaining isolates represented 1.9—-17.8% of any one species (Fig. 2). All isolates were fast growing on SNA and MEA, producing abun- dant aerial mycelia, and scarce numbers of sclerotia, chlamydospores or fruiting structures in 3—4 w. Phylogenetic analyses Sequence data were generated for all 107 isolates, which were approximately 250 bp for the ACT gene region, 660 bp for the CVDA, 430 bp for the H/S3, 1000 bp for the RPB2, 500 bp for the TEFI, and 560 bp for the TUB2. For the phylogenetic analyses of each individual data set, the HKY+G model was selected for ACT, the GTR+G model for CMVDA, the GTR+G for H/S3, the TIM2ef+G for RPB2, the TPM1uf+G for TUB2, and the TPM3uf+I+G for TEFI. The ML tree for each individual gene region with bootstrap support values of ML and posterior probabilities of BI are pre- sented in Suppl. material 1. The combined sequence data set used in the phylogenetic analyses included 191 ingroup taxa and 3 315 characters, including alignment gaps. Concatenated sequence alignments of the six gene regions together with closely related Calonectria species were deposited in Zenodo (10.5281/zenodo.7195911). Topologies of the trees result- ing from the ML and BI analyses were concordant and showed similar phylogenetic relationships between taxa. The ML tree with bootstrap support values for the ML and the posterior probabilities obtained from BI is presented in Fig. 3. Isolates considered in this study were all in the Prolate Group (Liu et al. 2020) and resided in either the C. brassicae, C. candelabrum or C. pteridis species complex. The majority of the isolates resided in the C. brassicae species complex. Fifty-eight isolates in this complex clustered in three different clades. Of these, 32 isolates grouped in the same clade with the ex-type isolate of C. parvispora, 19 isolates clustered together with C. brachiatica, and seven with C. pini. In the C. candelabrum species complex, 40 isolates clustered in three groups. Of these, four isolates grouped together with C. colombiana, 34 isolates with C. spathulata, and two isolates resided in a well-supported clade (ML/BI = 100/1.00) distinct from any known species in this complex and thus represent a novel taxon. The remaining nine isolates residing in the C. pteridis species complex were closely related to C. gordoniae but formed an independent clade (ML/BI = 100/1.00) distinct from C. gordoniae, as well as other species in this complex. ‘These isolates represent an undescribed taxon in Calonectria. Taxonomy Based on phylogenetic analyses and morphological observations, isolates collected from soils in Eucalyptus plantations and their adjacent native vegetation in Colombia represented five previously described species, namely, C. brachiatica, C. colombiana, 24 Nam Q. Pham et al. / MycoKeys 94: 17-35 (2022) TEF1+TUB2+HIS3+CMDA+ACT+RPB2 CMW 49483 CMW 49484 CMW 49646 CMW 49490 CMW 49477 7210-52 CMW 49645 CMW 49485 CMW 49643 CMW 49478 CMW 49476 CMW 49479 C. parvispora CBS 111465° C. parvispora CMW 30981 CMW 49471 CMW 49498 CMW 49468 - CMW 49486 pes C. parvispora CMW 49469 77") CMW 49497 CMW 49647 CMW 49765 CMW 49766 CMW 49508 */0.9911 CMW 49507 CMW 49506 CMW 49641 CMW 49481 98/1.00/4] CMW 49489 CMW 49488 CMW 49482 CMW 49480 CMW 49472 CMW 49491 C. brachiatica CMW 25298" C. brachiatica CMW 25302 C. brachiatica CMW 25307 CMW 49763 87/1-09F7 CMW 49764 */0.947" CMW 49762 CMW 49496 CMW 49474 CMW 49741 CMW 49751 fs 2 cMw 49734 C. brachiatica 99/1.00) CMW 49470 CMW 49492 CMW 49750 CMW 49475 CMW 49501 igs cMW 49642 CMW 49749 CMW 49473 CMW 49487 CMW 49740 CMW 49739 C. brassicae CBS 111869° C. pseudobrassicae CBS 134662" 100/1.00' ¢_ pseudobrassicae CBS 134661 100/1.00 C. orientalis CMW 20273 100/1.00 i 79/0.99 71/0.90. 77/0.90 97/1.00 CALONECTRIA BRASSICAE SPECIES COMPLEX C. orientalis CMW 20291' C. paraensis CBS 134669" C. paraensis LPF 429 C. pini CBS 125523 CMW 49505 84/") | CMW 49504 CMW 49502 CMW 49503 92/1.00] CMW 49500 CMW 49510 C. pini CMW 31209" CMW 49499 C. clavata CMW 30994 100/1.00! C. clavata CMW 23690° 100/1.00f C. gracilis CBS 111807" 100/1.00 C. gracilis CBS 111284 C. quinqueramosa CBS 134654" C. quinqueramosa CBS 134655 98/1.00] C. pseudoecuadoriae CBS 111402' 100/1.00 C. robigophila CBS 134652" */"] C. robigophila CBS 134653 C. duoramosa LPF 453 100/1.00'— C. duoramosa CBS 134656" C. octoramosa CBS 111423" */1.00| [ C. ecuadoriae CMW 23677" */0.98 C. pauciphialidica CMW 30980° */4 C. ecuadoriae CBS 111706 96/0.99' C. ecuadoriae CBS 114164 93/1.0D-] 95/1.00 */1.00 77/099 100/1.00} 73/1.00 Figure 3. Phylogenetic tree based on maximum likelihood (ML) analysis of a combined DNA data set of ACT, CMDA, HIS3, RPB2, TEFI and TUB2 sequences for Calonectria spp. Bootstrap values = 70% for ML analyses and posterior probabilities values > 0.90 obtained from Bayesian inference (BI) are indicated at the nodes as ML/BI. Bootstrap values < 70% or probabilities values < 0.90 are marked with “*”, and nodes lacking the support values are marked with “-”. Isolates representing ex-type material are marked with “I”. Curvicladiella cignea (isolate CBS 109167 and CBS 109168) represents the outgroup. Soil-borne Calonectria spp. from Colombia */0.9 74/1.00 99/1.00} */0,99 88/1.00} 94/1 100/1.00) 94/1.00 78/1.00| 95/- 100/0.974+—] 00 95/1.00 | C. glaebicola CBS 134852" *f4 C. glaebicola CBS 134853 */. C. eucalypticola CBS 134847" 90/1.00 C. eucalypticola CBS 134846 2 C. venezuelana CBS 111052" 99/0.9a| C. candelabrum CMW 31000 C. candelabrum CMW 31001 C. brasiliana CBS 111485 C. brasiliana CBS 111484" C. metrosideri CBS 133603" C. metrosideri CBS 133604 72/-}100/1.0g C. putriramosa CBS 111449° C. putriramosa CBS 111470 C. putriramosa CBS 111477 C. pseudometrosideri CBS 134845" 100/1.00" C. pseudometrosideri CBS 134843 C. brassiana CBS 134856 100/0.97" C. brassiana CBS 134855" C. piauiensis CBS 134851 100/1.00°— C. piauiensis CBS 134850" CMW 49752" 100/1.00° CMW 49753 100/1.00] C, colombiana CBS 115638 76/100 C. colombiana CBS 115127" 100/1.00-.y CMW 49720 */4.00) "CMW 49721 CMW 49716 CMW 49717 C. brevistipitata CBS 110837 98/1.00L] C. brevistipitata CBS 110928 100/100 C. brevistipitata CBS 115671" 100/1.00 C. pauciramosa CBS 138824" C. pauciramosa CMW 2151 100/1.00) C. nemuricola CBS 134837" C. nemuricola CBS 134838 100/1.001_1C. silvicola CBS 135237" 100/1.00' C. silvicola CBS 134836 100/1.00) C, pseudospathulata CBS 134841" C. pseudospathulata CBS 134840 C. fragariae CBS 133607' 100/1.00| C. fragariae LPF 141.2 C. fragariae LPF 141.1 CMW 49746 CMW 49730 CMW 49747 CMW 49731 CMW 49748 __ | CMW 49728 “I CMW 49729 CMW 49774 CMW 49733 CMW 49732 CMW 49745 CMW 49718 CMW 49719 CMW 49775 CMW 49771 100/1.00|| CMW 49727 CMW 49773 CMW 49723 CMW 49767 CMW 49761 C. spathulata CMW 16744" CMW 49744 CMW 49743 CMW 49758 */-| C. spathulata CBS 112513 CMW 49742 CMW 49756 CMW 49760 CMW 49754 CMW 49755 CMW 49757 CMW 49759 CMW 49724 CMW 49725 CMW 49726 “/-" CMW 49772 A /- 99/0.9 C. colombiana 86/1.00} 88/4 C. spathulata 93/0.91| 80/*] C. amazonica CBS 116250" 90/1.00" C. amazonica CBS 116242 100/1.00} 100/1.00} 100/1.00 79/0.92| 100/1.00 100/1.00 95/1.00| 100/100 100/1.00,| CMW 49787 0.01 Figure 3. Continued. C. amazonica CBS 115486 C. amazonica CBS 116271 C. amazonica CBS 115438 C. amazonica CBS 115440 C. pseudopteridis CBS 163.28" C. pteridis CBS 111793" C. terricola CBS 116247" C. ovata CMW 30979 C. ovata CMW 16724" C. pseudovata CBS 134675 98/1.00" C. pseudovata CBS 134674" C. gordoniae CMW 23694" CMW 49782 CMW 49783 CMW 49784 CMW 49785 ms a Calonectria guahibo sp. nov. CMW 49788 CMW 49789 CMW 49790 CMW 49791" Curvicladiella cignea CBS 109168 100/1.00'‘Cyrvicladiella cignea CBS 109167" | Calonectria exiguispora sp. nov. CALONECTRIA PTERIDIS CALONECTRIA CANDELABRUM SPECIES COMPLEX SPECIES COMPLEX 25 C. parvispora, C. pini and C. spathulata, and two novel species. One of these novel taxa resided in the C. candelabrum species complex and the other in the C. pteridis species complex. Descriptions for these species are provided as follows. 26 Nam Q. Pham et al. / MycoKeys 94: 17—35 (2022) Calonectria exiguispora N.Q. Pham, Marinc. & M.J. Wingf., sp. nov. MycoBank No: 846456 Figs 4, GA, B Etymology. “exiguus” (Latin) = small + “spora” (Latin) = spores, referring to the small macroconidia produced by this species. Diagnosis. Phylogenetically close to C. piauiensis and C. brassianae but differs in having smaller macroconidia. Type. Cotompia: Risaralda, Quinchia. Soils in Eucalyptus plantation. August 2016. C.A. Rodas. (Holotype PRU(M) 4501, stored in a metabolically inactive state; ex-holotype CMW 49752, CMW-IA 160). GenBank: OP796405 (ACT); OP822275 (CMDA); OP822382 (HIS3); OP822489 (RPB2); OP822168 (TEFI): OP822596 (TUB2). Description. Sexual morph not observed. Conidiophores scarce on SNA, consisting of conidiogenous apparatus and stipes, branched or simple. Stipes infre- quent, elongated, septate, 75-273 um long, 2-5 um wide near base, tapering towards apex, simple or occasionally dichotomously branched, mostly being part of conid- iogenous apparatus; vesicles terminal, slightly inflate to ellipsoidal, 2-5 um wide. Conidiogenous apparatus hyaline, simple or branched in 1-3 (—4) tiers, uncommon- ly developing from stipes; main axis upright, septate, 20-275 x 3-7 um; branches doliiform to cylindrical, primary branches 8-39 x 3-7 um, secondary branches 8—24 x 2-6 um, tertiary branches 10—23 x 2—5 um, quarternary branches 10-14 x 3-4 um. Conidiogenous cells holoblastic, hyaline, discrete, cylindrical to tapered above, often constricted near base, with periclinal thickening, 8—20 x 3-5 (11.8 = 2.71 x 3.2 £0.5) um. Macroconidia hyaline, cylindrical, round at apex, 1-septate, septum sub-median or median, guttulate, 21-40 x 3-4 (30.9 + 4.09 x 3.5 + 0.23) um. Chlamydospores present, scarce, in clumps or in chains. Mega- and microconidia not observed. Colonies on 2% MEA in the dark for 6 d, white on surface, pale luteous in re- verse, with moderate amount of aerial mycelium, with entire edges. Optimal growth temperature at 25 °C reaching 65.2 mm in 6 d, followed by 20 °C (57.3 mm), 15 °C (39.8 mm), 10 °C (19.7 mm), 5 °C (8.2 mm), no growth at 30 °C and 35 °C. Colonies kept at 30 °C and 35 °C being relocated to 25 °C for another 6 d revived (30 °C) and showed no growth (35 °C). Colonies on 2% MEA in the dark for 30 d, white to umber on surface, umber to dark brick in reverse, with flat mycelia. Distribution. Colombia. Material examined. Cotomsia: Risaralda, Quinchia. Soils in Eucalyptus sp. plan- tation. August 2016. C.A. Rodas. (PRU(M) 4502, stored in a metabolically inactive state; culture CMW 49753, CMW-IA 161). Notes. Calonectria exiguispora is a member of the C. candelabrum species complex (Liu et al. 2020). It shares some characteristics with other species in the complex, such as 1-septate macroconidia and ‘ellipsoidal to obpyriform’ shape vesicle. However, it can be distinguished from most species in the complex by its smaller conidial dimensions (21-40 x 3-4 um, avg. 30.9 x 3.5 um) except for C. brevistipitata (29-35 x 3-4 um, avg. 31 x 3.5 um, isolated from Mexican soil) and C. stipitate (27-37 x 3-6, avg. 32 x 4 um, Soil-borne Calonectria spp. from Colombia 27 A conidiophores formed on SNA B stipes and vesicles C conidiogenous apparatus D chlamydospores E conidia (x 400) F conidia (x1 000). Scale bars: 100 um (A); 50 um (D, E); 25 um (C); 10 um (B, F). isolated from Colombian Eucalyptus sp.) (Lombard et al. 2016). Nevertheless, these two species are distantly related to C. exiguispora (Fig. 3). Recently Liu et al. (2020) reduced C. stipitata to synonymy with C. spathulata, the conidial dimensions of which range from 48—100 x 4—6 um (avg. 80 x 6 um). They regarded the smaller conidial dimensions of C. ‘stipitata” as representing intraspecific variation. Calonectria exiguispora is phyloge- netically closely related to C. piauiensis and C. brassianae, which were isolated from soils associated with Eucalyptus brassiana trees in Brazil (Alfenas et al. 2015). These two spe- cies, however, have much larger conidial dimensions: C. piauiensis (38-60 x 3—5 um, avg. 49 x 4.5 um) and C. brassianae (35-65 x 3-5 um, avg. 53 x 4 um) (Alfenas et al. 2015). It can be differentiated from its most closely related species by sequences of ACT, CMDA, HIS3, RPB2, TEFI and TUB2 gene regions. Calonectria guahibo N.Q. Pham, Marinc. & M.J. Wingf., sp. nov. MycoBank No: 846457 Figs’>.6E, Etymology. Name refers to the indigenous people, Guahibo, native to Vichada, Colombia. Diagnosis. Closely related to C. gordoniae but differs in having smaller macroconidia. Type. Cotompia: Vichada, Cumaribo. Soils in Eucalyptus plantation. August 2016. C.A. Rodas. (Holotype PRU(M) 4503, stored in a metabolically inactive 28 Nam Q. Pham et al. / MycoKeys 94: 17-35 (2022) state; ex-holotype CMW 49791, CMW-IA 162). GenBank: OP796480 (ACT); OP822350 (CMDA); OP822457 (HIS3); OP822564 (RPB2); OP822243 (TEFI); OP822671 (TUB2). Description. Sexual morph not observed. Conidiophores scarce on SNA, com- posed of conidiogenous apparatus and stipes. Stipes part of conidiogenous appara- tus, elongated, septate, 81-223 um long, 2—5 um wide near base, tapering towards apex, simple, infrequently branched; vesicles slightly inflated to clavate, 2-5 um wide. Conidiogenous apparatus hyaline, branched irregularly in 2—3 (—4) tiers; main axis upright, septate, 25-83 x 4—6 um; branches doliiform to cylindrical, primary branches 11—23 x 4—6 um, secondary branches 7—16 x 3—5 pm, tertiary branches 9-11 x 3-4 um. Conidiogenous cells holoblastic, hyaline, discrete, cylindrical to ovoid, tapering to- wards apex, with perclinal thickening, 6-12 x 2-4 (9.3 = 1.46 x 3.0 = 0.52) um. Macroconidia hyaline, cylindrical with round ends, 1-septate, straight, septum me- dian or sub-median, 26-42 x 3-4 (31.7 + 3.59 x 3.2 + 0.19) um. Chlamydospores present in clumps or in chains. Mega- and microconidia not observed. Colonies on 2% MEA after 6 d in the dark, growing circular, with fluffy aerial mycelia, above white to pale luteous towards centre, reverse luteous to umber towards centre. Optimal growth temperature at 30 °C reaching 61 mm, followed by 25 °C (57.5 mm), 20 °C (48.3 mm), 15 °C (21.8 mm), and no growth at 5, 10, and 35 °C. Colonies kept at 5, 10, and 35 °C revived after being relocated to 25 °C. Colonies on 2% MEA in the dark for 30 d, with cottony mycelia filled entire Petri dish, above saf- fron to umber with patches of white, reverse dark brick to sepia. Figure 5. Micrographs of Calonectria guahibo sp. nov. (ex-holotype: CMW 49791 = CMW-IA 162). A conidiophores formed on SNA B stipes and vesicles C conidiogenous apparatus D chlamydospores E conidia (x 400) F conidia (x1 000). Scale bars: 100 um (A); 50 um (D); 25 um (C, E); 10 um (B, F). Soil-borne Calonectria spp. from Colombia 29 95°C ee ies: °C in the dark for 6 d (A, C, E, G) and 30 d (B, E, F, H) at its optimum temperature A, B CMW 49752 (ex-holotype) C, D CMW 49753 E, F CMW 49791 (ex-holotype) G, H CMW 49782. Distribution. Colombia. Material examined. Cotomsia: Vichada, Cumaribo. Soils in Eucalyptus sp. plan- tation. August 2016. C.A. Rodas, CMW 49782. Notes. Calonectria guahibo forms part of the C. pteridis species complex as a sister taxon to C. gordoniae. Calonectria gordoniae was reported from Florida, USA, caus- ing leaf spots and blotches on loblolly bay (Gordonia lasianthus) and is known to produce macroconidia (45-81 x 4—6 um, avg. 61.7 x 5 um) and microconidia (20- 42 x 3-4 um, avg. 32.5 x 3.6 um) (Leahy et al. 2000). Leahy et al. (2000) reported slightly curved conidia which were not observed in C. guahibo. Calonectria guahibo can be distinguished by its smaller conidia (26-42 x 3-4 um, avg. 31.7 x 3.2 um) from other closely related species, i.e. C. ovata (50-110 x 4-6 um, avg. 70 x 5 pm) (Victor et al. 1997), C. pseudovata (55-50 x 4-7, avg. 69 x 5 um) (Alfenas et al. 2015), and C. terricola (40-53 x 3-6 um, avg. 46 x 4.5 um) (Lombard et al. 2016). It can be dif- ferentiated from its most closely related species by sequences of ACT, CMDA, HIS3, RPB2, TEF1 and TUB2 gene regions. Discussion A relatively large number of Calonectria species were discovered from soils collected in Eucalyptus plantations in four forestry regions of Colombia. All of the isolates were identified based on DNA sequence comparisons for six gene regions and supported by morphological characteristics. Seven species residing in three species complexes were identified. These include five previously described species, C. brachiatica, C. parvispora and C. pini in the C. brassicae species complex, and C. colombiana and C. spathulata 30 Nam Q. Pham et al. / MycoKeys 94: 17—35 (2022) in the C. candelabrum species complex and two novel taxa for which the names C. exiguispora and C. guahibo have been provided. Calonectria parvispora was one of the most commonly isolated species (29.9%) and was recovered from two forestry regions (Fig. 1). This species has previously been found in soils collected from Brazil and Colombia (Marin-Felix et al. 2017), but this is the first record of this species from soils associated with Eucalyptus. Interestingly, C. brachiatica and C. pini in the C. brassicae species complex were also found, which were previously isolated from Pinus cuttings displaying collar and root rot symptoms in Colombian nurseries (Lombard et al. 2009; Lombard et al. 2010a). Calonectria pini was previously collected in Valle da Cauca (Lombard et al. 2010a), and its appearance in this study suggests that it has a relatively wide distribution in Colombia. Calonectria exiguispora, described in this study, has extended the total number of species of the C. candelabrum species complex to 20 (Liu et al. 2020; Sanchez-Gonzalez et al. 2022). In addition, two previously described species in the C. candelabrum spe- cies complex, C. colombiana and C. spathulata, were also found. The latter species rep- resented the majority of the isolates (31.8%). ‘This is relevant because C. spathulata is a well-known pathogen commonly associated with leaf and shoot blight on Eucalyptus, and it has been reported from plantations in tropical regions of South America (Crous and Kang 2001; Crous 2002; Rodas et al. 2005). In this study, C. spathulata was also isolated from soils collected in natural rainforests surrounding Eucalyptus plantations in Risaralda, where the first outbreak of the disease occurred. It is possible that it is native to this area, but further studies, including those at a population genetics level, would be required to resolve that question. Calonectria guahibo represents a new addition to the C. pteridis species complex, which now includes eight species (Liu et al. 2020), all of which have 1-septate mac- roconidia and clavate or ovate vesicles. Calonectria guahibo appears to have a limited distribution, with all isolates obtained from soils collected in plantations in Vichada, and interestingly, it was the only species found in this region. Although the C. pteridis species complex incorporates some of the most important pathogens of Eucalyptus (Crous 2002; Graga et al. 2009; Alfenas et al. 2013, 2015), nothing is known regard- ing the pathogenicity of the newly described C. guahibo. Many previous reports of Calonectria spp. are considered to be of dubious significance because identifications were mostly based on morphology. It is now well-recognised that multi-gene markers together with morphological comparisons are required to identify these fungi with confidence. Consequently, this study has provided a more comprehen- sive understanding of the species diversity and distribution of Calonectria in Colombian Eucalyptus plantations. This should contribute to the establishment of an effective man- agement strategy for the diseases caused by these fungi in plantations and nurseries. Results of previous investigations and the present study have shown that soils asso- ciated with commercially propagated Eucalyptus spp. in tropical and subtropical regions represent a niche that is remarkably rich in species of Calonectria (Alfenas et al. 2015; Lombard et al. 2015; Li et al. 2017; Pham et al. 2019, 2022; Wu and Chen 2021). New species of these important fungi will most likely emerge when more extensive Soil-borne Calonectria spp. from Colombia el surveys are extended for the remaining areas in Colombia in the future. Further studies should also be conducted to determine the relative importance of the many Calonectria spp. residing in the soils associated with Eucalyptus plantations in the country. Acknowledgements We acknowledge the financial support of the DST/NRF Centre of Excellence in Plant Health Biotechnology (CPHB), South Africa. We are also grateful to members of the staff of SmurfitKappa Colombia for assistance in collecting soil samples. 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Journal of Fungi 7(2): 73. https://doi.org/10.3390/jof7020073 Supplementary material | Phylogenetic tree based on maximum likelihood (ML) analysis of individual gene region (ACT, CMDA, HIS3, TUB2, TEF1 and RBP2) Authors: Nam Q. Pham, Seonju Marincowitz, ShuaiFei Chen, Carlos A. Rodas, Michael J. Wingfield Data type: (word document) Copyright notice: This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODDbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited. Link: https://doi.org/10.3897/mycokeys.94.96301.suppl1 Soil-borne Calonectria spp. from Colombia 35 Supplementary material 2 Collection details and GenBank accessions of isolates included in the phyloge- netic analyses Authors: Nam Q. Pham, Seonju Marincowitz, ShuaiFei Chen, Carlos A. Rodas, Michael J. Wingfield Data type: table (excel document) Copyright notice: This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODDbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited. Link: https://doi.org/10.3897/mycokeys.94.96301.suppl2