A peer-reviewed open-access journal ZooKeys 1099: | 11—121 (2022) fs Bee eA Tee #ZooKeys https:/ / ZOO keys. pensoft.net Launched to accelerate biodiversity research Two new species of the subgenus Reticularisus (Lepidoptera, Limacodidae, Rhamnosa) from China, with a checklist of the genus Rhamnosa Fixsen, 1887 Jun Wu', Ting-Ting Zhao', Hui-Lin Han'?? I School of Forestry, Northeast Forestry University, Harbin, 150040, China 2. Key Laboratory of Sustainable Forest Ecosystem Management, Ministry of Education, Northeast Forestry University, Harbin, 150040, China 3 Northeast Asia Biodiversity Research Center, Northeast Forestry University, Harbin, 150040, China Corresponding author: Hui-Lin Han (hanhuilin@aliyun.com) Academic editor: Andrew Mitchell | Received 5 October 2021 | Accepted 31 January 2022 | Published 3 May 2022 http://zoobank.org/8 18 DEAD3-4D0A-4D62-A990-C3188D8D7CB6 Citation: Wu J, Zhao T-T, Han H-L (2022) Two new species of the subgenus Reticularisus (Lepidoptera, Limacodidae, Rhamnosa) from China, with a checklist of the genus Rhamnosa Fixsen, 1887. ZooKeys 1099: 111-121. https://doi. org/10.3897/zookeys.1099.76163 Abstract Two new species of the subgenus Reticularisus Wu, Wu & Han, 2022 of the genus Rhamnosa Fixsen, 1887, Rhamnosa (Reticularisus) chenjuni sp. nov. and Rh. (R.) mangshanensis sp. nov., are described from the provinces of Hunan and Guangdong, China. The adults and genital structures of the new species and similar examined species are illustrated. A checklist of the genus is provided. Keywords Guangdong, Hunan, slug caterpillar moths, taxonomy, Zygaenoidea Introduction The genus Rhamnosa Fixsen, 1887 was erected based on the type species Rh. angulata Fixsen, 1887 from “Korea”. Since then, approximately ten new species have been described and reported (Fixsen 1887; Hering 1931, 1933; Matsumura 1931; Okano and Copyright Jun Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 112 Jun Wu etal. / ZooKeys 1099: 111-121 (2022) Pak 1964; Wu 2008; Wu and Fang 2009). Solovyev and Witt (2009) divided Rhamnosa into two subgenera, Rhamnosa Fixsen, 1887 and Caniodes Matsumura, 1927, based on the external features and the male genitalia. Later, Solovyev and Dubatolov (2015) clarified the exact taxonomic position of some species and provided a distribution map for Rh. angulata Fixsen, 1887. Solovyev (2017) established a third subgenus, Rhamnopsis Matsumura, 1931, for the endemic species R/. arizanella (Matsumura, 1931) from Taiwan. However, RA. arizanella was misplaced in the subgenus Rhamnosa by Wu et al. (2022), which is corrected in this paper. A fourth subgenus, Reticularisus Wu, Wu & Han, 2022, was established based on overall appearance and male genitalic characters. This subgenus contains two species: R/. (R.) henanensis Wu, 2008 and Rh. (R.) shierbeihoua Wu, Wu & Han, 2022 (Wu et al. 2022). To date, the genus included nine described species belonging to four subgenera, all of which have been recorded in China. In this study, two new species of the subgenus Reticularisus, Rh. (R.) chenjuni sp. nov. and Rh. (R.) mangshanensis sp. nov., collected from the Hunan and Guangdong provinces of China, are described. Materials and methods ‘The specimens were collected with a 220V/450W mercury vapour lamp and DC black light lamps at Mangshan National Nature Reserve and Nanling National Forest Park, respectively in the Hunan and Guangdong provinces of China. Standard methods for dissection and preparation of the genitalia slides were used (Kononenko and Han 2007). The specimens were photographed using a Nikon D700 camera, whereas the genitalia slides were photographed with an Olympus photo microscope and processed using the Helicon Focus software and Adobe Photoshop CS6. All type materials of the new species are deposited in the collection of the Northeast Forestry University (NEFU), Harbin, China. Material from the National Zoological Museum of China, Institute of Zoology, Chinese Academy of Sciences, Beijing, China (IZCAS) was also examined in this study. Taxonomic account Genus Rhamnosa Fixsen, 1887 Rhamnosa Fixsen, 1887: 339. Type species: Rhamnosa angulata Fixsen, 1887, by mono- typy. Type locality: “Korea”. Caniodes Matsumura, 1927: 91. Type species: Caniodes takamukui Matsumura, 1927. Type locality: “Formosa” (Horisha). Rnamnopsis Matsumura, 1931: 101. Type species: Rhamnopsis arizanella Matsumura, 1931. Type locality: “Formosa” (Arisan). Two new species of the subgenus Reticularisus from China jas Subgenus Reticularisus Wu, Wu & Han, 2022 Reticularisus Wu, Wu & Han, 2022: 138. Type species: Rhamnosa henanensis Wu, 2008, by original designation. Type locality: Henan Province, China. Notes. The subgenus is characterized by the forewing being pale yellow in ground col- our and covered with reddish-brown scales. The antemedial and postmedial lines are entire, not parallel, straight or slightly curved, darkish, running from the wing margin near the apex to the inner margin. The venation of the forewing is usually of an obvi- ous dark brownish red colour. The species are not sexually dimorphic; the females are usually slightly larger, with filiform or slightly bipectinated antennae. The male genitalia are diagnostic: apical part of juxta with massive numbers of tiny spines; basal part flat, with a pair of lateral processes that can be strongly sclerotized or not; saccus visible or simply present; valva without basal processes; aedeagus slender and always more or less spiral-shaped near the coecum. Rhamnosa (Reticularisus) chenjuni sp. nov. http://zoobank.org/ DF5 1 CFAB-D023-45C3-8B5 1-AOEF7CBOD55F Figg lo? 7 slid Holotype. ¢, Curna, Hunan Province, Chenzhou City, Yizhang County, Mangshan National Nature Reserve, Jiangjunzhai scenic spot, 30.VII-7.VIII.2021, leg. J. Wu and Q. Lin, genit. prep. WuJ-583-1 (NEFU). Paratypes. 17, 49, same date as for holotype, genit. prep. WuJ-582-1, WuJ- 584-2, WuJ-585-2 (NEFU). 24, Cuina, Guangdong Province, Shaoguan City, Ruy- uan County, Nanling National Forest Park, 24—27.V.2021, leg. MR. Li and G. Fu, genit. prep. WuJ-594-1, WuJ-595-1 (NEFU). Diagnosis. The new species Rh. chenjuni sp. nov. (Figs 1, 2) can be distinguished from the other three species (Figs 3-6) in the subgenus Reticularisus by the forewing patterns. The antemedial line of the forewing is barely visible in the region near the apex and does not intersect with the subterminal line, but in Rb. mangshanensis sp. nov. (Figs 3, 4), Rh. shierbeihoua (Fig. 5), and Rh. henanensis (Fig. 6), the antemedial lines are entire and intersect with the subterminal lines at the wing margin near the apex. In the male genitalia, Rb. chenjuni sp. nov. (Fig. 7) is most similar to the other new species Rb. mangshanensis sp. nov. (Fig. 8), but the diagnostic features are the short and stout gnathos, the lateral processes of the juxta strongly sclerotized and gradually diverging into 3—7 long, acuate spines, and the saccus is small and triangular rather than tongue-shaped. However, in Rh. mangshanensis sp. nov. the enathos is slender and curved at the middle; the lateral processes of the juxta bear a strongly sclerotized, long, hook-shaped process, and the saccus is tongue-shaped. R/. chenjuni sp. nov. differs from Rh. shierbeihoua (Fig. 9) and Rh. henanensis (Fig. 10) by the following characteristics of the male genitalia: the sacculus of the valva is wavy; 114 Jun Wu et al. / ZooKeys 1099: 111-121 (2022) the juxta bears a pair of lateral processes that are strongly sclerotized and gradually diverging into several long spines; the saccus is visible, small, triangular. However, in Rh. shierbeihoua, the sacculus of the valva is smoothly arc-curved, the lateral processes of juxta are short, not sclerotized, covered with massive numbers of spinules, and the saccus is not visible. In RA. henanensis, the sacculus of the valva is straight, the juxta bears a pair of sawblade-shaped and strongly sclerotized lateral processes, and the saccus is short and broad. In the female genitalia, Rb. chenjuni sp. nov. (Fig. 11) differs from Rh. mangshanensis sp. nov. (Fig. 12) by its strongly swollen genital chamber, highly modified lamella postvaginalis, less spiraled ductus bursae, larger corpus bursae, and the upper position of the signum. Figures 1-6. Adults of Rhamnosa (Reticularisus) spp. | Rh. chenjuni sp. nov., male, holotype (NEFU) 2 Rh. chenjuni sp. nov., female, paratype (NEFU) 3 R/. mangshanensis sp. nov., male, holotype (NEFU) 4 Rh. mangshanensis sp. nov., female, paratype (NEFU) 5 Rd. shierbeihoua Wu, Wu & Han, 2022, male, holotype, Guizhou, China (NEFU) 6 RA. henanensis Wu, 2008, male, holotype, Henan, China (IZCAS). Scale bar: 1 cm. Two new species of the subgenus Reticularisus from China 115 Description. Adult (Figs 1, 2). Forewing length 11-13 mm, wingspan 23- 29 mm in male (13-15 mm and 29-34 mm in female). Head brown; labial palpus short, brown; antennae bipectinated almost to the apex in male, filiform in female. Forewing ground colour ochreous to pale brown, with two distinct, slightly sinuous, dark brown antemedial and subterminal lines running from costal margin near apex, and reaching inner margin at ca. 1/3 and 2/3 distance from the wing base, respec- tively; antemedial line barely visible near apical region; a conspicuous dentiform tuft located at middle of inner margin; fringe ochreous with black terminally. Hindwing pale yellow, mixed with a little brown. Scales on legs ochreous to pale yellow. Abdo- men pale yellow. Male genitalia (Fig. 7). Uncus triangular, with a strongly sclerotized apical spur. Gnathos hook-shaped, slightly thinner terminally. Valva of almost equal width, up- per half part covered with dense hairs; sacculus obviously waved; cucullus broad and rounded. Juxta flattened, rounded, slightly divided apically; lateral process plate- shaped, bearing a strongly sclerotized process gradually diverging into 3—7 (normally of 5) long, acuate spines. Saccus visible, as a small triangle. Aedeagus slender, slightly spiral-shaped near coecum; cornuti of vesica not obvious. Female genitalia (Fig. 11). Papillae anales ear-shaped, covered with dense setae on surface, margins with a dorsal and ventral lobe and several deep clefts in the middle. Postvaginal plate strongly sclerotized, nearly square. Anterior apophysis short; posterior apophysis long and slender, ca. 4x length of anterior apophysis. Ostium bursae strongly sclerotized. Ductus bursae very long, strongly spiral-shaped in basal part. Corpus bursae pear-shaped, covered with dense spines on the outside and with a spindle-shaped signum that is strongly sclerotized and almost as long as corpus bursae. Distribution (Fig. 13). China (Hunan: Mangshan; Guangdong: Nanling). Etymology. The species name is dedicated to Mr. Jun Chen, who works in the Mangshan State-owned Forestry Administration in Hunan Province, China. He was of great assistance to us when we were collecting in Mangshan National Nature Reserve. Remarks. This new species differs clearly in appearance from the other three spe- cies in the subgenus Reticularisus, mainly in having antemedial line not visible near the apical region and forewing lacking distinctive marks other than the antemedial and submarginal lines. It shares some similarities with Rb. (Rhamnosa) hatita (Druce, 1896); however, because it highly matches the characters of the subgenus Reticularisus for the male genitalia, i.e., valva without a basal process, juxta with a pair of distinct lateral processes, saccus visible, and aedeagus spiraled near coecum, it is provisionally placed in this subgenus. These moths fly from late May to August. The specimens were collected by 220V/450W mercury light and DC black light at 570—1,265 m a.s.L; the collecting site in Hunan province is located close to mixed coniferous and broad-leaved forests (Figs 14, 16). 116 Jun Wu etal. / ZooKeys 1099: 111-121 (2022) Figures 7-12. Genitalia of Rhamnosa (Reticularisus) spp. 1 Rh. chenjuni sp. nov., male, holotype (NEFU) 8 RA. mangshanensis sp. nov., male, holotype (NEFU) 9 RA. shierbeihoua Wu, Wu & Han, 2022, male, holotype, genit. slide WuJ-301-1 (NEFU) 10 RA. henanensis Wu, 2008, male, holotype (IZCAS) I 1 RA. chenjuni sp. nov., female, paratype, genit. slide WuJ-585-2 (NEFU) 12 Rh. mangshanensis sp. nov., female, paratype, genit. slide WuJ-593-2 (NEFU). Scale bars: 1 mm. Two new species of the subgenus Reticularisus from China By Rhamnosa (Reticularisus) mangshanensis sp. nov. http://zoobank.org/72F98AFB-557A-49B0-BB6A-68FAB1D4009F Figs 3, 4, 8, 12 Holotype. ¢, Curna, Hunan Province, Chenzhou City, Yizhang County, Mangshan National Nature Reserve, Jiangjunzhai scenic spot, 30.VII—7.VIII.2021, leg. J. Wu and Q. Lin, genit. prep. WuJ-581-1 (NEFU). Paratypes. 275, same date as for holotype, genit. prep. WuJ-579-1, WuJ-580- 1 (NEFU); 1¢, 19, Cuina, Guangdong Province, Shaoguan City, Ruyuan County, Nanling National Forest Park, 24—27.V.2021, leg. MR. Li and G. Fu, genit. prep. WuJ-592-1, WuJ-593-2 (NEFU). Diagnosis. Three of the species in the subgenus Reticularisus, Rh. mangshanensis sp. nov. (Figs 3, 4), Rhamnosa. shierbeihoua (Fig. 5), and Rh. henanensis (Fig. 6), are very similar in appearance. Rh. mangshanensis sp. nov. can be distinguished from Rh. shierbeihoua by the point of emergence of the two oblique antemedial and subterminal lines (running from the costal margin near the apex in Rb. mangshanensis sp. nov. but from the outer margin near the apex in RA. shierbeihoua), and by the ground colour of the body (pale brownish-yellow in Rb. mangshanensis sp. nov. but pale yellow in Rh. shierbeihoua). However, it is hard to distinguish it from the k/. henanensis only by its external appearance. The male genitalia of R4. mangshanensis sp. nov. (Fig. 8) are clearly distinguishable from those of Rh. shierbeihoua (Fig. 9) and Rh. henanensis (Fig. 10). The gnathos of Rh. mangshanensis sp. nov. is slender and up-curved at an obtuse angle at the middle, the sacculus of the valva is distinctly waved, and the lateral processes of the juxta are strongly sclerotized with a long, slender, hook-shaped, basally serrated and terminally forked process. In Rh. shierbeihoua (Fig. 9) and Rh. henanensis (Fig. 10) the gnathos are shorter and thicker, the sacculi are straight or smoothly arc-curved, the lateral processes of the juxta are short, nearly plate-shaped, without a long slender process at apex. The differences in external appearance and genitalia between Rb. mangshanensis sp. nov. and Rh. chenjuni sp. nov. are listed under the latter species. Description. Adult (Figs 3, 4). Forewing length 11-12 mm, wingspan 24—27 mm in male (14 mm and 29 mm in the single studied female). Head dark brown; la- bial palpus short, dark brown; male antennae bipectinated almost to apex, female antennae also bipectinated but extremely thinner than male’s. Thorax brownish yel- low; patagium reddish-brown; tegula brownish-yellow. Forewing ground colour pale brownish-yellow; costal margin dark brown to black near apex; two distinct, oblique, dark brown antemedial and subterminal lines running from costal margin near apex to inner margin: antemedial line straight, reaching to ca. 1/3 from wing base, subter- minal line slightly curved towards outer margin, reaching to ca. 2/3 from wing base; a mixed brownish-yellow and dark brown dentiform tuft is located between these two lines along the inner margin; venation visible in forewing, brown, veins at margins of cell dark brown; fringe dark brown to black. Hindwing pale yellow; fringe dark brown at apex, remainder pale yellow. Scales on legs brown to pale yellow. Abdomen brownish-yellow. 118 Jun Wu et al. / ZooKeys 1099: 111-121 (2022) Male genitalia (Fig. 8). Uncus triangular, elongated, with a strongly sclerotized apical spur. Gnathos slender, hook-shaped, up-curved at an obtuse angle at middle. Valva broad at base; sacculus obviously waved; cucullus slightly narrower, rounded. Juxta highly modified, covered with massive numbers of tiny spines in upper part; ba- sal part with a pair of strongly sclerotized, long, slender, hook-shaped lateral processes, with a row of teeth at base and terminally forked. Vinculum narrow. Saccus strongly sclerotized, tongue-shaped. Aedeagus slender, slightly spiral-shaped near coecum, scle- rotized at apex; vesica with dense, tiny cornuti. Female genitalia (Fig. 12). Papillae anales ear-shaped, covered with dense setae on surface, margins with several small clefts. Anterior apophysis short but robust, pointed apically, with an obvious tongue-shaped process next to it; posterior apophysis long and slender, slightly enlarged subapically, ca. 4.5x length of anterior apophysis. Genital chamber strongly sclerotized and obviously swollen, with a pair of rounded processes below it. Lamella postvaginalis highly modified, oval-shaped, densely covered with short hairs, with a pair of small hairy processes. Ductus bursae long, membranous, thick and strongly spiral-shaped at base. Corpus bursae large, oval-shaped, densely covered with tiny sclerotized flecks, with a spindle-shaped, strongly sclerotized, erect signum situated in upper 2/3. Distribution (Fig. 13). China (Hunan: Mangshan; Guangdong: Nanling). Etymology. The new species is named after its type locality, the Mangshan Na- tional Nature Reserve of Hunan Province, China. Remarks. These moths fly from late May to August. The specimens were col- lected by 220V/450W mercury light and DC black light at 570-1,265 m a.s.L.; the collecting site in Hunan province is close to mixed coniferous and broad-leaved forests (Figs 15, 16). Checklist of species of the genus Rhamnosa Fixsen, | 887 Subgenus Rhamnosa Fixsen, 1887 Rh. (R.) angulata Fixsen, 1887 Rh. (R.) hatita (Druce, 1896) = Rh. angulate kwangtungensis Hering, 1931 = Rh. (R.) kwangtungensis Hering, 1931 Rh. (R.) dentifera Hering & Hopp, 1927 Rh. (R.) convergens Hering, 1931 Subgenus Caniodes Matsumura, 1927 Rh. (C.) uniformis (Swinhoe, 1895) = Cania notodonta Hampson, 1897 = Caniodes takamukui Matsumura, 1927 = Rh. uniformis rufina Hering, 1931 Rh. (C.) uniformoides Wu & Fang, 2009 Two new species of the subgenus Reticularisus from China ES) Subgenus Rhamnopsis Matsumura, 1931 Rh. (R.) arizanella (Matsumura, 1931) = Rhamnopsis arizanella Matsumura, 1931 = Rh. arizanella (Matsumura, 1931) Subgenus Reticularisus Wu, Wu & Han, 2022 Rh. (R.) henanensis Wu, 2008 Rh. (R.) chenjuni sp. nov. Rh. (R.) mangshanensis sp. nov. Rh. (R.) shierbeihoua Wu, Wu & Han, 2022 Figures 13-16. 13 collecting sites of the two new species: Hunan Province, Mangshan National Nature Reserve (red dot a); Guangdong Province, Nanling National Forest Park (red dot b) 14, 15 adult male, living habitus: 14 RA. chenjuni sp. nov. 15 Rh. mangshanensis sp. nov. 16 the biotope of these two new species in Hunan is close to a mixed coniferous and broad-leaved forest. 120 Jun Wu et al. / ZooKeys 1099: 111-121 (2022) Discussion The genus Rhamnosa includes four subgenera with a total of eleven species, all 11 species are recorded in China based on the literature. The subgenus Reticularisus was established for its unique characteristics of the forewing and male genitalia. In the four known species of the subgenus, the antemedial and subterminal lines of the forewing always intersect near the apex (the antemedial line of RA. (R.) chenjuni sp. nov. is not visible anteriorly so the two lines do not intersect, but the two lines would cross if both visible), and the aedeagus is always more or less spiral-shaped near the coecum, so these two characters may be considered as apomorphies of the subgenus. Acknowledgements The present study was supported by the National Natural Science Foundation of Chi- na (No. 31872261) and the Fundamental Research Funds for the Central Universities (No. 2572021DJ08, 2572019CP11). We sincerely thank Mr. Jun Chen, who works at the Mangshan State-owned Forestry Administration in Hunan Province, for his great assistance during collecting in Mangshan National Nature Reserve; and the colleagues from our laboratory for collecting a part of the material of these two new species. References Fixsen C (1887) Lepidoptera aus Korea. In: Romanoff NM (Ed.) Mémoires sur les lépidopteéres, Tom III. St. Pe’tersbourg, Imprimerie de M. M. Stassuléwitch, 233-356. [pl. 15] Hering M (1931) Limacodidae (Cochliopodidae). In: Seitz A (Ed.) Die Gross-Schmetterlinge der Erde. Vol. 10. Alfred Kerner Verlag, Stuttgart, 665-728. Hering M (1933) Familie: Limacodidae (Cochliopodidae). In: Seitz A (Ed.) Die Gross-Schmet- terlinge der Erde. Supplement 2. Die Palaearktischen Spinner und Schwarmer. Alfred Kernen Verlag, Stuttgart, 201-209. Kononenko VS, Han HL (2007) Atlas Genitalia of Noctuidae in Korea (Lepidoptera). In: Park K-T (Ed.) Insects of Korea (Series 11). Junhaeng-Sa, Seoul, 464 pp. Matsumura S (1927) New species and subspecies of moths from the Japanese Empire. The jour- nal of the College of Agriculture, Tohoku Imperial University, Sapporo, Japan 19(1): 1-91. Matsumura S (1931) Descriptions of some new genera and species from Japan, with a list of species of the family Cochlidionidae. Insecta Matsumurana 5(3): 101-116. Okano M, Pak SW (1964) A revision of the Korean species of the family Heterogeneidae (Lepidoptera). Annual report of the College of Leberal Arts. University of Lwate 22: 1-10. Solovyev AV (2017) Limacodid moths (Lepidoptera, Limacodidae) of Taiwan, with descriptions of six new species. Entomological Review 97(8): 1140-1148. https://doi.org/10.1134/ S0013873817080140 Two new species of the subgenus Reticularisus from China 18 Solovyev AV, Dubatolov VV (2015) Rhamnosa angulata Fixsen, 1887 (Lepidoptera, Limacodi- dae) — a new record of slug moths for the Russian fauna, with a review of species recorded near the Russian border. Evraziatskii Entomologicheskii Zhurnal 14(1): 63-69. [in Rus- sian with English summary] Solovyev AV, Witt ThJ (2009) The Limacodidae of Vietnam. Entomofauna (Supplement 16): 33-229. Wu CS (2008) A new species of the genus Rhamnosa Fixsen from Henan Province (Lepidoptera: Limacodidae). In: Shen XC, Lu CT (Eds) The fauna and taxonomy of insects in Henan, vol. 6: Insects of Baotianman National Nature Reserve, China Agricultural Science and Technology Press, Beijing, 7—8. [in Chinese] Wu CS, Fang CL (2009) A review of Rhamnosa from China (Lepidoptera: Limacodidae). Oriental Insects 43(1): 253-259. https://doi.org/10.1080/00305316.2009.10417586 Wu J, Wu CS, Han HL (2022) Reticularisus Wu, Wu & Han, a new subgenus of the genus Rhamnosa Fixsen, 1887 from China, with description of a new species (Lepidoptera: Lima- codidae). SHILAP Revista de Lepidopterologia 50(197): 137-144.