ZooKeys 207: 65-78 (20 | 2) A peer-rev iewed open-access journa I
doi: 10.3897/zookeys.207.3419 RESEARCH ARTICLE #7Z,00Ke y

www.zookeys.o rg Launched to accelerate biodiversity research

Three new species of Atopsyche Banks
(Trichoptera, Hydrobiosidae) from Brazil

Allan P. M. Santos'*3t, Ralph W. Holzenthal?*

| Departamento de Zoologia, Instituto de Biologia, Universidade Federal do Rio de Janeiro, RO. box 68044,
Rio de Janeiro, RJ 21941-971, Brazil 2 CAPES Foundation, Ministry of Education of Brazil, Brasilia, DF
70040-020, Brazil 3 Department of Entomology, University of Minnesota, 219 Hodson Hall, 1980 Folwell
Ave., St. Paul, Minnesota, 55108, USA

T urn:lsid:zoobank.org:author:FDBB2390-430F-4769-A3A3-E8DD1 CF36EBC
* urn:lsid:zoobank. org:author:C27A I C49-F372-4232-A49C-B8B5F753CC70

Corresponding author: Allan P M. Santos (a.santos@ufrj.br)

Academic editor: Christy Geraci | Received 25 May 2012 | Accepted 20 June 2012 | Published 11 July 2012
urn:lsid:zoobank. org:pub:F391D3A7-0F39-4818-87D7-5AFDID9I20C3B

Citation: Santos APM, Holzenthal RW (2012) Three new species of Atopsyche Banks (Trichoptera, Hydrobiosidae) from
Brazil. ZooKeys 207: 65-78. doi: 10.3897/zookeys.207.3419

Abstract

Three new species of Atopsyche Banks (Hydrobiosidae) from Brazil are described and illustrated: Atopscyhe
(Atopsaura) blahniki sp. n., Atopsyche (Atopsyche) parauna sp. n., and Atopsyche (Atopsaura) galharada sp.
n. Additional illustrations of the male genitalia of A. urumarca Schmid are provided, including its popula-
tional variation. Also, we provide new state records for 2 species: A. (Atopsyche) urumarca from Sao Paulo,
and A. (Atopsaura) plancki Marlier from Santa Catarina.

Keywords
Atlantic forest, Atopsyche, Brazil, caddisfly, Hydrobiosidae, Neotropics

Introduction

Atopsyche Banks constitutes the most diverse genus in the family Hydrobiosidae, with
over 120 described species (Holzenthal and Cressa 2002, Holzenthal et al. 2007).
The genus occurs from the southwestern United States to northern Argentina, but it

Copyright A.PM. Santos, R.W. Holzenthal. This is an open access article distributed under the terms of the Creative Commons Attribution License
3.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

66 Allan P M. Santos & Ralph W. Holzenthal / ZooKeys 207: 65—78 (2012)

is replaced by other genera of Hydrobiosidae in the Chilean subregion (Holzenthal
and Cressa 2002). Schimd (1989) provided a world revision of the family Hydro-
biosidae, including comments on the classification and phylogeny of Atopsyche and
descriptions of several new species. Currently, 19 species are recorded from Brazil, of
which only 2 were recorded after Schmid (1989): A. chirihuana Schmid, originally
described from Ecuador; and A. erigia Ross, originally described from Mexico (Blah-
nik et al. 2004). Following Schmid (1989), Atopsyche species are divided into three
subgenera, based on features of the male genitalia: Atopsaura Ross, Atopsyche Banks,
and Dolochorema Banks.

In this paper, we describe 3 new species of Atopsyche from southeastern Brazil.
These additional species bring the number of known caddisflies species from Brazil
to 569, but many species of this and other genera remain undescribed. In addition,
we illustrate variations in the male genitalia of A. urumarca Schmid, and provide
new state distributional records of A. urumarca from Sao Paulo and A. plancki
Marlier from Santa Catarina.

Material and methods

Morphological terminology used in this paper follows that presented by Schmid
(1989). The lactic acid method (Blahnik et al. 2007) was used for specimen prepara-
tion. Genital structures were observed and illustrated with a compound microscope,
equipped with a drawing tube. Pencil sketches were scanned and placed into an
Adobe Illustrator (v. 13.0.0, Adobe Systems Inc.) document to produce a digital
illustration. Descriptions provided for new species were made using the software
DELTA (Dallwitz et al. 1999). Holotypes are deposited in the Museu de Zoologia,
Universidade de Sao Paulo, Sao Paulo, Brazil (MZSP). Paratypes and other mate-
rial examined are deposited in the MZSP and also in the University of Minnesota
Insect Collection, St. Paul, Minnesota, USA (UMSP), the National Museum of
Natural History, Smithsonian Institution, Washington DC, USA (NMNH), and
the Colecao Entomoldgica Prof. José Alfredo Pinheiro Dutra, Universidade Federal
do Rio de Janeiro, Rio de Janeiro, Brazil (DZRJ).

Taxonomy

Atopsyche (Atopsaura) blahniki sp. n.

urn:lsid:zoobank.org:act: 17398E47-24AC-45A6-8FEF-CF2B3A9BBFE2
http://species-id.net/wiki/Atopsyche_blahniki

Figs 1, 4, 7

Diagnosis. ‘This new species is most similar to Atopsyche zernyi Flint, also described from
Brazil. Both species have an apical process on the first article of the inferior append-

Three new species of Atopsyche Banks (Trichoptera, Hydrobiosidae) from Brazil 67

Figure |. Atopsyche (Atopsaura) blahniki sp. n. Male genitalia: A lateral B parapod, lateral C parapod and
preanal appendage, dorsal D inferior appendage, ventral E phallic apparatus, lateral F phallic apparatus,
dorsal. Abbreviations: aed aedeagus fil filipod inf app inferior appendage par parapod phal phallotheca
pt app preanal appendage proc proctiger IX abdominal segment IX.

age as long as the second article, and a phallotheca with long paired processes, apically
upturned and narrow. However, Atopsyche blahniki sp. n. differs from A. zernyi by the
narrower parapod, longer filipod (exceeding length of parapod), and in the apices of the
paired processes from the phallotheca, which bear several spines on their lateral edges.

68 Allan P M. Santos & Ralph W. Holzenthal / ZooKeys 207: 65-78 (2012)

Male. Forewing length 5.5 mm (n=1). Overall body color brown; antennal scape
brown, with long brown setae, pedicel brown, basal flagellomeres yellow, apical flag-
ellomeres brown; setae of palps yellow; frons and vertex of head with long, erect
brown and whitish setae; legs yellowish brown, coxae and femora of forelegs darker
brown. Forewings brown; erect setae on veins forming irregular pattern of alternate
dark brown and yellow setae; apex of wing with fringe of alternating patches of dark
brown and yellow setae. Forewing venation complete (Fig. 4A); R1 branched; stem
of fork I about twice its length; fork II long, sessile; stem of fork III equal to its
length; fork IV long, sessile; stem of M almost straight between m-cu crossvein and
first fork of M; fork V long, narrow; Cu2 long, converging near fused anal veins;
apex of fused anal veins very short. Hind wing (Fig. 4B) with Sc and R1 fused api-
cally; forks I, III, and V present, the first with a short stem, the last long with a short
stem, stem of fork II equal to its length; forks I] and IV absent; M3+4 not reaching
wing margin; Cu2 long and almost straight; 1A long and sinuate. Nygmas indistinct
in both wings. Tergum II with pair of prominent glands at posterolateral margin
(Fig. 7A); tergum III with pair of prominent gland at anterolateral margin, lined at
opening with minute setae (Figs 7A, 7B). Sternum V with pair of small, rounded
glands on anterolateral margins (Fig. 7C). Sterna VI and VII with prominent spine-
like ventral processes on posteromesal margins.

Male genitalia. Segment IX, in lateral view, long (Fig. 1A). Parapod narrow,
simple, with small dorsolaterally directed tooth-like projections and short, stout se-
tae (Figs 1A, 1B, 1C). Filipod long, slender, with elongate setae along length, apex
attenuate (Fig. 1A). Preanal appendage short, rounded, setose (Figs 1A, 1C). Inferior
appendage, first article long, slightly widened apically, otherwise relatively narrow
and of uniform width, with subtriangular apical process, nearly equaling second
article in length (Figs 1A, 1D); second article small, with subacute apex and small
rounded carina ventrally (Figs 1A, 1D). Proctiger, in lateral view, broadly widened
apically, with truncate apical margin, covered with minute setae, apicodorsal mar-
gin with long setae (Fig. 1A). Phallic apparatus complex (Fig. 1E, 1F); phallotheca
broadly rounded basally, with narrow ventral process articulating with inferior ap-
pendages (Fig. 1E); posteriorly divided into long, paired processes (Fig. 1F); pro-
cesses apically upturned and narrow, covered with minute setae and bearing spines
on lateral edges (Figs 1E, 1F); aedeagus an elongate, stout, spine-like structure, with
slight ventral curvature near base (Fig. 1E).

Holotype male. BRAZIL: Rio de Janeiro: Cachoeiras de Macacu, Rio Souza,
16°26.567'S, 42°37.957'W, 150 m, 16.iii.1996, Holzenthal, Rochetti & Oliveira
(pinned) (UMSP000031906) (MZSP).

Paratypes. BRAZIL: Rio de Janeiro: same data as holotype, 23 females (pinned)
(17 females, UMSP; 2 females, NMNH; 2 females, MZSP; 2 females, DZRJ).

Etymology. This new species is named in honor of Dr. Roger Blahnik, who identi-

fied this new species.

Three new species of Atopsyche Banks (Trichoptera, Hydrobiosidae) from Brazil 69

Atopsyche (Atopsyche) parauna sp. n.
urn:lsid:zoobank.org:act:D90947CD-CF4E-44EA-98 19-D47E65CCF7F6
http://species-id.net/wiki/Atopsyche_parauna

Figs 2,356

Diagnosis. This new species is most similar to A. jaba Blahnik and Gottschalk, de-
scribed from Costa Rica. These two species share a similar mesal process on the first ar-
ticle of the inferior appendage and the complex phallotheca, with paired processes pos-
teriorly. Atopsyche parauna sp. n. can be distinguished from A. jaba and other Atopsyche
species by the broad parapod, in lateral view, with the dorsolateral margin serrate and
with a midlateral spinose projection, whereas in A. jaba, the parapod has 2 prominent
spines. In addition, the second article of the inferior appendage is shorter and slightly
hooked in the new species, and the phallotheca is posteriorly divided into 2 long,
paired processes, the dorsal one birfucate apically and the ventral one curved mesally.
Male. Forewing length 5.0—5.5 mm (n=10). Overall body color brown; antennal
scape brown, with long stramineous setae, pedicel brown, basal flagellomeres yellow, api-
cal flagellomeres brown; setae of palps yellow; frons and vertex of head with long, erect
brown and whitish setae; legs yellowish brown, coxae of all legs darker brown. Forewings
brown; erect setae on veins forming distinct mottled pattern of alternate dark brown and
yellow setae, with dark brown setae along costal margin; apex of wing with fringe of al-
ternating patches of dark brown and yellow setae. Forewing venation complete (Fig. 5A);
R1 branched; stem of fork I equal to its length; fork II long, sessile; stem of fork HI equal
to its length; fork IV long, sessile; stem of M slightly curved between m-cu crossvein and
first fork of M; fork V long, narrow; Cu2 long, converging near fused anal veins, crossvein
near apex forming small cell on posterior margin of wing; apex of fused anal veins very
short. Hind wing (Fig. 5B) with Sc and R1 fused apically; forks I, HI, and V present,
the first with a short stem, the last long with a short stem, stem of fork III longer than
its length; forks I] and IV absent; M3+4 reaching wing margin; Cu2 long and almost
straight; 1A long and strongly curved, with row of elongate setae along its length. Nyg-
mas indistinct in both wings. Terga III and IV with pair of prominent rounded glands at
anterolateral margin, lined internally with minute setae (Fig. 8A). Sternum V with pair
of small rounded glands on anterolateral margins, with a keel-like projection (Fig. 8B).
Sterna VI and VII with prominent spine-like ventral processes on posteromesal margins.
Male genitalia. Segment IX, in lateral view, short (Fig. 2A). Parapod broad, with
dorsolateral margin serrate, setose, and with small, midlateral spinose projection (Figs
2A, 2B). Filipod long, slender, with elongate setae along length, apex somewhat capi-
tate (Fig. 2A). Preanal appendage short, rounded, setose (Figs 2A, 2B). Inferior ap-
pendage, first article long, slightly constricted mesally, otherwise relatively narrow and
of uniform width, without apical process, but mesally with small process at midlength,
bearing short spines (Figs 2A, 2C); second article small, slightly hooked apically (Figs
2A, 2C). Proctiger, in lateral view, broadly widened apically, with angulate apical mar-

70 Allan PR M. Santos & Ralph W. Holzenthal / ZooKeys 207: 65-78 (2012)

Figure 2. Azopsyche (Atopsyche) parauna sp. n. Male genitalia: A lateral B parapod and preanal append-
age, dorsal C inferior appendage, ventral D phallic apparatus, lateral E phallic apparatus, dorsal.

gin, covered with minute setae, apicodorsal margin with long setae (Fig. 2A). Phallic
apparatus complex (Figs 2D, 2E); phallotheca broadly rounded basally, with short
rounded ventral process articulating with inferior appendages (Fig. 2D); posteriorly
divided into two long, paired processes, dorsal one longer and bifurcate apically, ven-
tral one curved mesally (Figs 2D, 2E); aedeagus an elongate, stout, spine-like structure,
with ventral curvature near base (Fig. 2D).

Three new species of Atopsyche Banks (Trichoptera, Hydrobiosidae) from Brazil a

Holotype male. BRAZIL: Minas Gerais: Rio Paratina, 3 km S Santana do Ria-
cho, 19°10.986'S, 43°43.485'W, 650 m, 11.xi.2001, Holzenthal, Paprocki, Blahnik
& Amarante (pinned) (UMSP000080716) (MZSP).

Paratypes. BRAZIL: Minas Gerais: same data as holotype, 2 males, 3 fe-
males (pinned) (UMSP); Cardeal Mota, Cachoeira Véu da Noiva, 19°18.912'S,
43°36.260'W, 800 m, 12.xi.2001, Holzenthal, Paprocki, Blahnik & Amarante, 2
males (pinned) (DZRJ); Rio Cipé, Cachoeira de Baixo, 19°20.553'S, 43°38.531'W,
750 m, 10.xi.2001, Holzenthal, Paprocki, Blahnik & Amarante, 3 males (pinned) (2
males, NMNH; 1 male, UMSP).

Etymology. This species is named after the river where the holotype was collected,
that means “black river” in the Tupi-guarani language.

Atopsyche (Atopsaura) galharada sp. n.
urn:lsid:zoobank.org:act:4664284A-65D7-4F52-8ADB-8E66C1EAF4C2
http://species-id.net/wiki/Atopsyche_galharada

Figs 3, 6, 9

Diagnosis. This is a distinctive new species in the genus, belonging to the A. longipennis
Ulmer group of the subgenus Atopsaura. Atopsyche galharada sp. n. resembles A. ayah-
uaca Schmid and A. plancki Marlier in the absence of filipods and in the simple phal-
lotheca. The new species can be distinguished from the others by the broader parapod
with 2 carinas bearing tooth-like processes. The new species and A. plancki are similar
in the short and broad first article of the inferior appendage, with an apical process, but
in the new species this process is longer and the second article of the inferior append-
age is broad in ventral view, and slightly hooked in lateral view. Also, A. galharada has
the proctiger with a broad lateral sclerotized projection, and the phallotheca posteriorly
divided into long, paired flangelike processes, broadest subapically, in dorsal view.
Male. Forewing length 6.5—-7.5 mm (n=23). Overall body color brown; antennal
scape light brown, with long stramineous setae, pedicel brown, basal flagellomeres
yellow, apical flagellomeres brown; setae of palps dark brown; frons and vertex of
head with long, erect stramineous setae; legs yellowish brown, coxae of all legs darker
brown. Forewings brown; erect setae on veins forming irregular pattern of alternate
dark brown and yellow setae, with dark brown setae along costal margin; apex of wing
with fringe of alternating patches of dark brown and yellow setae. Forewing venation
complete (Fig. 6A); R1 branched; stem of fork I equal to its length; fork II long, sessile;
stem of fork HI equal in length to stem of fork I; fork IV long, sessile; stem of M slight-
ly curved between m-cu crossvein and first fork of M; fork V long, narrow; Cu2 long,
converging near fused anal veins, crossvein near apex forming small cell on posterior
margin of wing; apex of fused anal veins very short. Hind wing (Fig. 6B) with Sc and
R1 fused apically; forks I, III, and V present, the first with a short stem, the last long
with a short stem, stem of fork III equal to its length; forks H and IV absent; M3+4
reaching wing margin; Cu2 long and almost straight; 1A long and sinuate. Nygmas

fib. Allan PR M. Santos & Ralph W. Holzenthal / ZooKeys 207: 65—78 (2012)

De: Sea Sa|

- eee:

wf

fom

—/ oo a ar ae
OILILLIILIEE Yorn:
a ke
re eee

3E

Figure 3. Azopsyche (Atopsaura) galharada sp. n. Male genitalia: A lateral B parapod and preanal append-
age, dorsal C inferior appendage, ventral D phallic apparatus, lateral E phallic apparatus, dorsal.

indistinct in both wings. Sternum V with pair of small rounded glands on anterolateral
margins, with a keel-like projection (Fig. 9). Sterna VI and VII with prominent spine-
like ventral processes on posteromesal margins.

Three new species of Atopsyche Banks (Trichoptera, Hydrobiosidae) from Brazil 73

iyo eV
eS IV 7 M3

M4
TATDA+3A Cu2 Cutb Cuta

4B

6B

Figures 4-6. Azopsyche spp. n. Male wing venation: Figure 4 A. (Atopsaura) blahniki sp. n. 4A forewing
4B hind wing. Figure 5 A. (Atopsyche) parauna sp. n. 5A forewing 5B hind wing. Figure 6 A. (Atopsaura)
galharada sp. n. 6A forewing 6B hind wing.

74 Allan PR M. Santos & Ralph W. Holzenthal / ZooKeys 207: 65—78 (2012)

Figures 7-9. Atopsyche spp. n. Abdominal glands: Figure 7 A. (Atopsaura) blahniki sp. n. TA terga II and
HI, lateral 7B tergum III, dorsal C sternum V, lateral. Figure 8 A. (Atopsyche) parauna sp. n. 8A terga
II and IV, dorsal 8B sternum V, ventral. Figure 9 A. (Atopsaura) galharada sp. n., sternum V, lateral.

Male genitalia. Segment IX, in lateral view, long (Fig. 3A). Parapod broad basally,
narrow apically, with two carinas bearing tooth-like processes along dorsolateral edges
and short, stout setae (Figs 3A, 3B). Filipod absent (Fig. 3A). Preanal appendage short,
rounded, setose (Figs 3A, 3B). Inferior appendage, first article short, uniformly broad,
with narrow apical process, nearly equaling second article in length (Figs 3A, 3C);
second article large, bulging basally and downturned apically, in ventral view, broad
(Figs 3A, 3C). Proctiger, in lateral view, uniformly wide, with broad lateral sclerotized
projection and truncate apical margin, covered with minute setae, apicodorsal margin
with long setae (Fig. 3A). Phallic apparatus simple (Figs 3D, 3E); phallotheca broadly
rounded basally, not articulating with inferior appendages (Fig. 3D); posteriorly di-

Three new species of Atopsyche Banks (Trichoptera, Hydrobiosidae) from Brazil 75

vided into long, paired flangelike processes, broadest subapically, in dorsal view (Fig.
3E); aedeagus an elongate, stout, spine-like structure, with strong basal loop (Fig. 3D).

Holotype male. BRAZIL: Sao Paulo: Campos do Jordao, Parque Estadual de
Campos do Jordao, Rio Galharada, 22°41.662'S, 45°27.783'W, 1530 m, 4—5.iii.1996,
Holzenthal & Guahyba (pinned) (UMSP000031880) (MZSP).

Paratypes. BRAZIL: Sao Paulo: same data as holotype, 2 females (pinned)
(MZSP); same data, except 22.i.1998, Holzenthal, Froehlich & Paprocki,17 fe-
males (pinned) (UMSP); same data, except 13-15.ix.2002, Blahnik, Prather, Melo,
Huamantinco, 1 male, 3 females (alcohol) (UMSP); Parque Estadual de Campos do
Jordao, Campo do Meio, 22°41.750'S, 45°29.448'W, 1500 m, 21.i1.1998, Holzen-
thal, Froehlich & Paprocki, 2 females (pinned) (UMSP); same data, except 6.iii.1996,
Holzenthal & Guahyba, 1 male, 2 females (pinned) (NMNH); Rio Casquilho, 3.4
km NE Parque Estadual de Campos do Jordao, 22°40.29'S, 45°27.87'W, 1550 m,
23.1.1998, Holzenthal, Froehlich & Paprocki, 18 males, 39 females (pinned) (15
males, 34 females, UMSP; 3 males, 5 females, DZRJ); Ribeirao do Casquilho, Bosque
Vermelho, ca. 5 km Parque Estadual de Campos do Jordao, 22°40'S, 45°27.5'W, 1435
m, 16.ix.2002, Blahnik, Prather, Melo & Huamantinco, 3 males (UMSP).

Etymology. This species is named after the river where holotype was collected.

Atopsyche (Atopsyche) urumarca Schmid
http://species-id.net/wiki/Atopsyche_urumarca
Figs 10

Atopsyche (Atopsaura) urumarca Schmid, 1989: 131 [type locality: Brazil, Serra do
Cipd, Rio Capivara; MZSP; male].

Diagnosis. Atopsyche urumarca Schmid was described from Minas Gerais state, Bra-
zil, and placed in the A. bolivari Banks group. According to Schimid (1989), A.
urumarca is most similar to A. pachacutec Schmid, especially in the form of the
parapod and the apex of the phallotheca, but they differ in the structure of the sec-
ond article of the inferior appendage. We have examined material from southeastern
Brazil, including the type locality, and we found an interesting populational varia-
tion in the male genitalia structure. This variation is noticeable in the shape of the
parapod and the apex of phallotheca. In all specimens, the parapod, in lateral view,
is broad basally, with an oblique, mesal U-shaped incision, but this incision is deep-
est in specimens collected in Ipoema, Minas Gerais state, and shallow in specimens
from Sao Paulo state; the parapod also can end in a narrow and rounded apex or
bear a small, dorsal spine. The phallotheca is broadly rounded basally, with 3 paired
processes posteriorly, the dorsal one long and broad, with small dorsal and ventral
projections at its apex. Some specimens have a small, additional spine-like process on
the posterior margin of this dorsal process, and the ventral margin can be rounded,

slightly pointed, or pointed and hooked apically.

76 Allan P M. Santos & Ralph W. Holzenthal / ZooKeys 207: 65—78 (2012)

“

‘ NI
\
ty ij
yi
an os :

10F

Figure 10. Azopsyche (Atopsyche) urumarca Schmd. Variation in male genitalia. A lateral, specimen from
Ipoema, Minas Gerais B—D parapod, lateral B specimen from Serra do Cipdé, Minas Gerais C specimen
from Morro do Pilar, Minas Gerais D specimen from Altindpolis, Sao Paulo E-G phallic apparatus, lat-

eral E specimen from Ipoema F specimen from Morro do Pilar G specimen from Altindpolis.

Material examined. BRAZIL: Minas Gerais: Serra do Cipd, Rio Capivara,
19°20.553'S, 43°38.531'W, 950 m, 11.ii.1998, Holzenthal & Paprocki, 2 males
(pinned) (UMSP); Serra do Cip6, trib. to Rio Capivara, 19°14.396'S, 43°34.939'W,
1000 m, 18.ii.1998, Holzenthal & Paprocki, 3 males (pinned) (MZSP); Rio Tanque,
ca. 12 km (rd) from Ipoema, 19°32.208'S, 43°26.878'W, 750 m, 16.v.1998,
Holzenthal & Paprocki, 2 males, 1 female (pinned) (DZRJ); Rio Santo Anténio,
downstream from Morro do Pilar, 19°08.134'S, 43°21.256'W, 530 m, 17.x.2000,
Paprocki & Ferreira, 1 male (pinned) (UMSP); P.E. de Sao Gongalo do Rio Preto,
Rio Preto, 18°07.841'S, 43°20.246'W, 791 m, 12.x.2000, Paprocki, Amarante, Sal-

Three new species of Atopsyche Banks (Trichoptera, Hydrobiosidae) from Brazil Ta,

gado, 1 male (alcohol) (UMSP); Sao Paulo: Altindpolis, Cachoeira dos Macacos,
20°55.390'S, 47°22.758'W, 758 m, 18.xi.2003, Holzenthal, Paprocki & Calor, 27
males, 15 females (pinned) (UMSP), 9 males, 1 female (alcohol) (DZRJ); Altindépo-
lis, Fazenda Sa0 Joao da Mata, Rio Baguassu, 21°00.588'S, 47°28.900'W, 745 m,
19-21.xi.2003, Holzenthal, Paprocki & Calor, 8 male, 17 females (pinned) (3 males,
5 females, NMNH; 5 males, 12 females, UMSP), 28 males (alcohol) (UMSP); Pe-
dregulho, Sitio Bruninho, 20°09.240'S, 47°30.704'W, 630 m, 17.xi.2003, Holzen-
thal, Paprocki & Calor, 1 male, 1 female (pinned) (UMSP); Pedregulho, Ribeirao
Sao Pedro, 20°09.113'S, 47°30.626'W, 617 m, 16.ix.2003, Holzenthal, Paprocki,
Calor, 1 male (alcohol) (UMSP).

Atopsyche (Atopsaura) plancki Marlier
http://species-id.net/wiki/Atopsyche_plancki

Atopsyche (Atopsaura) plancki Marlier 1964: 2 [type locality: Brazil, Sao Paulo; IRSNB;
male] - Blahnik et al. 2004: 4 [distribution; Brazil: Minas Gerais and Rio de Ja-

neiro states]

Material examined. BRAZIL: Santa Catarina: Parque Ecolégico Spitzkopf, confi.
Rio Ouro & Rio Caeté, 27°00.352'S, 49°06.693'W, 140 m, 25.xi.2003, Holzenthal,
Paprocki, Calor, 1 male (alcohol) (UMSP).

Acknowledgments

We are grateful to Dr. Roger Blahnik for assistance in the laboratory and important com-
ments on this manuscript. We also are grateful to Adolfo Calor, Adriano Melo, Claudio
Froehlich, and Henrique Paprocki for field assistance. APMS was supported by fellow-
ship (PDSE 0085/12-0) from CAPES Foundation (Coordenagao de Aperfeicoamento
de Pessoal de Nivel Superior), Ministry of Education of Brazil. This material is based on
work supported by National Science Foundation grant no. DEB 9971885.

References

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Blahnik RJ, Holzenthal RW, Prather A (2007) The lactic acid method for clearing Trichoptera
genitalia. In: Bueno-Soria J, Barba-Alvarez R, Armitage B (Eds) Proceedings of the XIIth
International Symposium on Trichoptera. The Caddis Press, Columbus, Ohio, 9-14.

Dallwitz MJ, Paine TA, Zurcher EJ (1999) User’s guide to the DELTA Editor. http://deltaint-
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