Karyotype characterization of planthopper species Hysteropterum albaceticum Dlabola, 1983 and Agalmatium bilobum (Fieber, 1877) (Homoptera: Auchenorrhyncha: Issidae) using AgNOR-, C- and DAPI/CMA3 -banding techniques

By

Kuznetsova, Valentina G.
Maryańska-Nadachowska, Anna
Nokkala, Seppo

Type

Article

Date of Publication

2009

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

3

Series / Issue

Issue: 2

Pages

111-123

Subjects

Agalmatium bilobum , Auchenorrhyncha , C-heterochromatin , chiasmata , DAPI/CMA3 -staining , Homoptera , Hysteropterum albaceticum , Issidae , NORs , polymorphism

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/compcytogen.v3i2.18

 

Abstract

Males of Hysteropterum albaceticum Dlabola, 1983 and Agalmatium bilobum (Fieber, 1877) display a chromosomal complement of 2n = 26 + X, which is a basic one of the tribe Issini (Issidae). In the present study, silver staining, C-banding and a base specific CMA3 -and DAPI-banding were used with the aim of identifying possible cytogenetic markers and distinguishing between karyotypes with the same chromosome number and no detectable inter-species differences in karyotype structure. We characterized the species studied in terms of the distribution and molecular structure of C-heterochromatin regions and the location of nucleolus organizing regions (NORs). The species are shown to differ considerably in the amount of heterochromatin, its distribution pattern along the karyotypes and its stain ability with DAPI and CMA3.