Karyotyping and single-gene detection using fluorescence in situ hybridization on chromosomes of Hydra magnipapillata (Cnidaria: Hydrozoa)

By

Anokhin, B. A. (Boris Aleksandrovich)
Hemmrich-Stanisak, G
Bosch, Thomas C. G.

Type

Article

Date of Publication

2010

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

4

Series / Issue

Issue: 2

Pages

97-110

Subjects

Hydra magnipapillata, karyotype, chromosomes, fluo

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/compcytogen.v4i2.41

 

Abstract

The fresh water polyp Hydra L., 1758 (Cnidaria, Hydrozoa) plays a key role as a model organism in modern evolutionary and developmental biology. A complete genome sequence has been published recently for Hydra magnipapillata Ito, 1947 and molecular data are rapidly accumulating in the literature, but little information is available on its chromosomes. In this study, an efficient fluorescence in situ hybridization (FISH) method is described for H. magnipapillata which not only allows identification of the chromosomes but also visualization of the location of individual genetic loci. Together with cDNA and genomic sequencing this may provide the foundation for increasingly precise genetic and physical mapping in this basal metazoan model organism.