Cytogenetic studies in four cultivated Amaranthus (Amaranthaceae) species

By

Bonasora, Marisa
Poggio, Lidia
Greizerstein, Eduardo

Type

Article

Date of Publication

2013

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

7

Series / Issue

Issue: 1

Pages

53-61

Subjects

Amaranthus , constitutive heterochromatin , DAPI-CMA3 , Fish , NORs

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/compcytogen.v7i1.4276

 

Abstract

In the present study, the chromosomes numbers were confirmed, 2n = 34 for Amaranthus cruentus Linnaeus, 1759, and 2n = 32 for A. hypochondriacus Linnaeus, 1753, A. mantegazzianus Passer, 1864, and A. caudatus Linnaeus, 1753. The distribution and variability of constitutive heterochromatin were detailed using DAPI-CMA3 banding technique. The position of the nucleolus organizer region (NOR) was observed using Ag-NOR banding (active loci) and fluorescent in situ hybridization (rDNA-FISH) in the four Amaranthus species. Variations in the amount of constitutive heterochromatin were detected both within the species and between them, with DAPI-CMA3 stain. One chromosome paira NOR was found in each studied accession, with exception of A. caudatus cv. EEA INTA Anguil. This accession presented four rDNA loci (FISH), being active two of them (Ag- banding).