Calcaridorylaimus castaneae sp. n. (Nematoda, Dorylaimidae) from Bulgaria with an identification key to the species of the genus

By

Nedelchev, Sevdan
Elshishka, Milka
Lazarova, Stela
Radoslavov, Georgi
Hristov, Peter
Peneva, Vlada

Type

Article

Date of Publication

2014

Original Publication

ZooKeys

Volume

410

Pages

41-61

Subjects

18S and D2-D3 rRNA genes , compendium , Morphology , taxonomy

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.410.6955

 

Abstract

An unknown species belonging to the genus Calcaridorylaimus Andrássy, 1986 was collected from the litter of broadleaf forests dominated by Castanea sativa Mill. and mixed with Quercus daleshampii Ten. and Fagus sylvatica L. on Belasitsa Mountain, south-western Bulgaria. Calcaridorylaimus castaneae sp. n. is characterised by its long body (1.4–2.1 mm), lip region practically not offset, vulva transverse, short odontostyle (14.5–16 µm) and tail (75.5–110.5 µm, c=14.7–23.6; c’=2.9–4.4) in females and 38–46 µm long spicules with small spur before their distant end in males. It is most similar to C. andrassyi Ahmad & Shaheen, 2004, but differs intransverse vs pore-like vulva and shorter spicules (38–46 µm vs 52–57 µm). An identification key to the species of the genus Calcaridorylaimus is proposed. Phylogenetic analyses were performed on 18S and D2-D3 expansion domains of 28S rRNA genes by Neighbor-Joining, Maximum Likelihood and Bayesian Inference methods. The phylograms inferred from 18S sequences showed closest relationships of the new species with some species belonging to the genus Mesodorylaimus. However, insufficient molecular data for members of both genera do not allow the phylogenetic relationships of Calcaridorylaimus and the new species described herein to be elucidated.