High-resolution gradient polyacrylamide electrophoresis of isozymes of the mosquito, Aedes aegypti.

Type

Article

Date of Publication

1985

Original Publication

Journal of the American Mosquito Control Association

Volume

1

Series / Issue

Issue: 3

Pages

353-356

Contributed by

American Mosquito Control Association

Notes

Author(s) - Novak RJ.Moss D.Byrd H.

 

Abstract

A procedure for high resolution electrophoresis of isozymes of the mosquito Aedes aegypti using nonlinear (2.5%-20%) polyacrylamide gradient gel slabs is described. Crude mosquito homogenates were electrophoresed on gradient and on homogeneous (7%) polyacrylamide gels and stained for esterase (EST), isocitrate dehydrogenase (IDH) and malate dehydrogenase (MDH). The resulting zymograms were compared to demonstrate the high resolving power of the polyacrylamide gradient gel system.

Notes

Author(s) - Novak RJ.Moss D.Byrd H.