A strain of Anopheles stephensi from Pakistan (MalR) was 8.7-fold resistant to malathion and 6.7-fold cross-resistant to phenthoate, but not to other carboxylesters; no cross-resistance to bendiocarb, fenitrothion, permethrin and carbaryl was detected. Resistance was not associated with elevated levels of general esterase activity, as determined by hydrolysis of naphth-l-yl acetate (alpha-NA), but was correlated with higher levels of malathion carboxylesterase (MCE) activity in vitro. The results suggest that a highly specific type of esterase mediated resistance, such as MCE, can best be detected by an enzyme specific assay rather than one for general esterase activity. A new, rapid and sensitive assay for mosquito MCE is given.
