Laboratory bioassay of the PG-14 isolate of Bacillus thuringiensis var. morrisoni (serotype 8a:8b) against early fourth instar larvae of 8 species of mosquitoes revealed a range of susceptibilities similar to the susceptibilities of these species to Bacillus thuringiensis var. israelensis (serotype 14). The most susceptible species were: Culex quinquefasciatus, Cx. salinarius, Anopheles albimanus and Aedes aegypti. The least susceptible species tested was An. quadrimaculatus. Separate bioassays of PG-14 against the four instars of Ae. aegypti demonstrated a strong negative correlation (R = -0.97) between larval age and susceptibility. Temperature significantly affected the stability of larvicidal toxin in aqueous suspensions of PG-14. Larvicidal activity of a bacterial suspension was nearly completely eliminated after 132 days of storage at 31 degrees C, but was essentially unchanged for those suspensions stored at 4 degrees C.
