Comparative efficacy of natural juvenile hormones extracted from Aphis craccivora and A. gosypii and 5 juvenoids, i.e., methoprene, Neporex, OMS 3007, OMS 3019 and DPE-28 on the development of Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus have been evaluated. OMS 3007, OMS 3019, DPE-28 and Neporex show species specific related activities, while methoprene and aphid extracts do not show such activity against these mosquito species. Treatment of mosquito eggs with an EC50 dose of these compounds caused mortality while an EC90 dose completely ceased adult emergence. The fecundity and fertility rates of mosquitoes emerged shows significant reduction (P less than 0.001) when treated with EC50 doses of all the compounds.