Heme peroxidase activity measured in single mosquitoes identifies individuals expressing an elevated oxidase for insecticide resistance.

Type

Article

Date of Publication

1997

Original Publication

Journal of the American Mosquito Control Association

Volume

13

Series / Issue

Issue: 3

Pages

233-237

Contributed by

American Mosquito Control Association

Notes

Author(s) - Brogdon WG.McAllister JC.Vulule J.

 

Abstract

Optimum conditions are described for a simple, rapid, microplate-based assay that indirectly measures the differences in oxidase levels between individual susceptible, resistant, or induced mosquitoes. A small proportion (0.01-0.1) of a single mosquito is used, allowing multiple replicates of the oxidase assay. Cytochrome C is used as a positive control. The levels of oxidase found in sample populations of pyrethroid-susceptible, pyrethroid-resistant, and phenobarbital-induced Anopheles albimanus mosquitoes are characterized with the assay.

Notes

Author(s) - Brogdon WG.McAllister JC.Vulule J.