A diagnostic polymerase chain reaction assay for species A and D of the Anopheles dirus (Diptera: Culicidae) species complex based on ribosomal DNA second internal transcribed spacer sequence.

Type

Article

Date of Publication

1998

Original Publication

Journal of the American Mosquito Control Association

Volume

14

Series / Issue

Issue: 4

Pages

385-389

Contributed by

American Mosquito Control Association

Notes

Author(s) - Xu X.Xu J.Qu F.

 

Abstract

A polymerase chain reaction assay based on differences in the internal transcribed spacer regions of ribosomal DNA was developed for distinguishing 2 members of the Anopheles dirus sibling species complex. This assay distinguished An. dirus species A from species D by producing diagnostic bands, 374 base pairs (bp) in length for species A and 663 bp in length for species D. Both laboratory colonies and field collections from Hainan and Yunnan provinces of China were identified with 100% accuracy.

Notes

Author(s) - Xu X.Xu J.Qu F.