Reduction in susceptibility to Brugia malayi of F2 progeny of Aedes togoi treated with ethyl methanesulfonate.

Type

Article

Date of Publication

1999

Original Publication

Journal of the American Mosquito Control Association

Volume

15

Series / Issue

Issue: 4

Pages

458-462

Contributed by

American Mosquito Control Association

Notes

Author(s) - Rodriguez PH.Castillon R.Wilson P.Benoit D.Nasr-Schirf D.

 

Abstract

The susceptibility to Brugia malayi infection was tested in F2 female progeny derived from male and female Aedes togoi treated with ethyl methanesulfonate (EMS). Three-day-old males and females were treated with 0.025, 0.050, and 0.075, 0.10, 0.15, or 0.20% EMS by allowing them to feed for 5 days on sugar cubes containing EMS and then mated at random. Percentage of susceptibility and mean number of infective larvae (L3) in F2 females were analyzed over a 2-wk period. Reductions in susceptibility were significant in the F2 populations arising from the 3 highest EMS concentrations. F2 infections were reduced by 80%, indicating that EMS-induced mutations affect loci associated with filarial nematode susceptibility.

Notes

Author(s) - Rodriguez PH.Castillon R.Wilson P.Benoit D.Nasr-Schirf D.