Polymerase chain reaction assay to identify all immature stages of two species of the Anopheles quadrimaculatus sibling species complex (Diptera: Culicidae).

Type

Article

Date of Publication

1999

Original Publication

Journal of the American Mosquito Control Association

Volume

15

Series / Issue

Issue: 4

Pages

573-575

Contributed by

American Mosquito Control Association

Notes

Author(s) - Rutledge CR.Wesson DM.Meek CL.

 

Abstract

A polymerase chain reaction assay that detects differences in the 2nd internal transcribed spacer of ribosomal DNA was tested as a means to identify all immature stages of 2 species in the Anopheles quadrimaculatus sibling species complex. The technique was successful in amplifying DNA from eggs, 1st-4th instars, and pupae of An. quadrilmaculatus Say and An. inundatus Reinert.

Notes

Author(s) - Rutledge CR.Wesson DM.Meek CL.