Use of Polymerase Chain Reaction Technique to Confirm VecTest™ Screening Results in Plasmodium falciparum and Plasmodium vivax VK 210 Laboratory-Infected Anopheles stephensi Mosquitoes

Type

Article

Date of Publication

2004

Original Publication

Journal of the American Mosquito Control Association

Volume

20

Series / Issue

Issue: 3

Pages

265-271

Contributed by

American Mosquito Control Association

Notes

Author(s) - Patricia D. Santos-Ciminera, Nicole L. Acheé, Gerald V. Quinnan, Jr., Donald R. Roberts

 

Abstract

We evaluated polymerase chain reaction (PCR) to confirm immunoassays for malaria parasitesin mosquito pools after a failure to detect malaria with PCR during an outbreak in which pools tested positiveusing VecTest™ and enzyme-linked immunosorbent assay (ELISA). We combined VecTest, ELISA, and PCR todetect Plasmodium falciparum and Plasmodium vivax VK 210. Each mosquito...

Notes

Author(s) - Patricia D. Santos-Ciminera, Nicole L. Acheé, Gerald V. Quinnan, Jr., Donald R. Roberts