Five Anophelines viz., Anopheles annularis, An. culicifacies, An. fluviatilis, An. stephensi and An. subpictus, were studied with respect to non-specific esterases using polyacrylamide gel electrophoresis. Male specific esterases were found in An. stephensi and An. subpictus and not in the other three Anophelines tested. In both the species, bands were localized in the accessory glands. In An. stephensi, the male specific esterase band appeared first in 18-20 hr old pupae while in An. subpictus they appeared in 0-1 hr old pupae. These bands persisted during the adult life of the male mosquitoes.
