Acquisition of the J. Pedro Duret mosquito collection by the Smithsonian Institution is reported. The collection contains more than 14,000 individually pinned and labeled adult mosquitoes, including 39 primary type specimens. It also includes some 7800 male genitalia preparations and 900 larval and pupal exuviae on microscope slides. A complete list of Duret species-group names is included, with label data for holotype specimens contained in the collection.