Evolutionary trends in the family Curimatidae (Characiformes): inferences from chromosome banding

Sampaio, Tatiane Ramos; Pires, Larissa; Venturelli, Natalia; Usso, Mariana Campaner; Rosa, Renata da; Dias, A. L.

doi:10.3897/CompCytogen.v10i1.6316

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Article: Evolutionary trends in the family Curimatidae (Characiformes): inferences from chromosome banding

Title

Evolutionary trends in the family Curimatidae (Characiformes): inferences from chromosome banding

By

Sampaio, Tatiane Ramos
Pires, Larissa
Venturelli, Natalia
Usso, Mariana Campaner
Rosa, Renata da
Dias, A. L.

Type

Article

Date of Publication

2016

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

Series / Issue

Issue: 1

Pages

77-95

Subjects

fluorochromes , heterochromatin , karyotype evolution , Pisces , rDNA

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/CompCytogen.v10i1.6316

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License Type URL: https://creativecommons.org/licenses/by/4.0/
Rights: https://biodiversitylibrary.org/permissions
Copyright Status: In copyright. Digitized with the permission of the rights holder.
Rights Holder: Copyright held by individual article author(s).

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expand Abstract

The family Curimatidae is a fish group usually considered chromosomally conserved in their diploid number. However, some studies show small changes in the karyotype microstructure, and the presence of B chromosomes, indicating a chromosomal diversification within the group, even if structural changes in the karyotypes are not visible. Few studies associate this trait with an evolutionary pattern within the family. This study aimed to characterize the karyotype, nucleolus organizer regions (NORs), and heterochromatin distribution of six species of Curimatidae of the genera Cyphocharax Fowler, 1906 and Steindachnerina Fowler, 1906: C. voga (Hensel, 1870), C. spilotus (Vari, 1987), C. saladensis (Meinken, 1933), C. modestus (Fernández-Yépez, 1948), S. biornata (Braga et Azpelicueta, 1987) and S. insculpta (Fernández-Yépez, 1948) and contribute data to a better understanding of the mechanisms involved in the chromosomal evolution of this group of fish. All specimens had 2n=54, m-sm, and B microchromosomes. Five species exhibited single NORs, except for S. biornata, which showed a multiple pattern of ribosomal sites. NORs were chromomycin A3 positive (CMA3+) and 4’-6-diamino-2-phenylindole (DAPI-) negative, exhibiting differences in the pair and chromosomal location of each individual of the species. FISH with 5S rDNA probe revealed sites in the pericentrometic position of a pair of chromosomes of five species. However, another site was detected on a metacentric chromosome of C. spilotus. Heterochromatin distributed both in the pericentromeric and some terminal regions was revealed to be CMA3+/DAPI-. These data associated with the previously existing ones confirm that, although Curimatidae have a very conservative karyotype macrostructure, NORs and heterochromatin variability are caused by mechanisms of chromosome alterations, such as translocations and/or inversions, leading to the evolution and diversification of this group of fish.