Cytogenetic study on antlions (Neuroptera, Myrmeleontidae): first data on telomere structure and rDNA location

By

Kuznetsova, Valentina G.
Khabiev, Gadzhimurad
Anokhin, B. A. (Boris Aleksandrovich)

Type

Article

Date of Publication

2016

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

10

Series / Issue

Issue: 4

Pages

647-656

Subjects

Acanthaclisis occitanica , Ascalaphidae , Distoleon tetragrammicus , fluorescence in situ hybridization , Libelloides macaronius , Myrmeleontidae , Neuroptera , Palpares libelluloides , rDNA , TTAGG

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/CompCytogen.v10i4.10775

 

Abstract

Myrmeleontidae, commonly known as “antlions”, are the most diverse family of the insect order Neuroptera, with over 1700 described species (in 191 genera) of which 37 species (in 21 genera) have so far been studied in respect to standard karyotypes. In the present paper we provide first data on the occurrence of the “insect-type” telomeric repeat (TTAGG)n and location of 18S rDNA clusters in the antlion karyotypes studied using fluorescence in situ hybridization (FISH). We show that males of Palpares libelluloides (Linnaeus, 1764) (Palparinae), Acanthaclisis occitanica (Villers, 1789) (Acanthaclisinae) and Distoleon tetragrammicus (Fabricius, 1798) (Nemoleontinae) have rDNA clusters on a large bivalent, two last species having an additional rDNA cluster on one of the sex chromosomes, most probably the X. (TTAGG)n - containing telomeres are clearly characteristic of P. libelluloides and A. occitanica; the presence of this telomeric motif in D. tetragrammicus is questionable. In addition, we detected the presence of the (TTAGG)n telomeric repeat in Libelloides macaronius (Scopoli, 1763) from the family Ascalaphidae (owlflies), a sister group to the Myrmeleontidae. We presume that the “insect” motif (TTAGG)n was present in a common ancestor of the families Ascalaphidae and Myrmeleontidae within the neuropteran suborder Myrmeleontiformia.