Evaluating mating compatibility within fruit fly cryptic species complexes and the potential role of sex pheromones in pre-mating isolation

By

Juárez, María Laura, 1980-
Devescovi, Francisco
Břízová, Radka
Bachmann, Guillermo
Segura, Diego Fernando
Kalinová, Blanka
Fernández, Patricia
Ruiz, María Josefina
Yang, Jianquan
Teal, Peter E. A.
Caceres, Carlos
Vreysen, M. J. B.
Hendrichs, Jorge
Vera, M. Teresa

Type

Article

Date of Publication

2015

Original Publication

ZooKeys

Volume

540

Pages

125-155

Subjects

 Ceratitis fasciventris ,  Ceratitis rosa , Anastrepha fraterculus , Bactrocera dorsalis , Ceratitis anonae , field cages , species delimitation , Tephritidae

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.540.6133

 

Abstract

The study of sexual behavior and the identification of the signals involved in mate recognition between con-specifics are key components that can shed some light, as part of an integrative taxonomic approach, in delimitating species within species complexes. In the Tephritidae family several species complexes have received particular attention as they include important agricultural pests such as the Ceratitis fasciventris (Bezzi), Ceratitis anonae (Graham) and Ceratitis rosa Karsch (FAR) complex, the Bactrocera dorsalis (Hendel) complex and the Anastrepha fraterculus (Wiedemann) complex. Here the value and usefulness of a methodology that uses walk-in field cages with host trees to assess, under semi-natural conditions, mating compatibility within these complexes is reviewed, and the same methodology to study the role of chemical communication in pre-mating isolation among A. fraterculus populations is used. Results showed that under the same experimental conditions it was possible to distinguish an entire range of different outcomes: from full mating compatibility among some populations to complete assortative mating among others. The effectiveness of the methodology in contributing to defining species limits was shown in two species complexes: A. fraterculus and B. dorsalis, and in the case of the latter the synonymization of several established species was published. We conclude that walk-in field cages constitute a powerful tool to measure mating compatibility, which is also useful to determine the role of chemical signals in species recognition. Overall, this experimental approach provides a good source of information about reproductive boundaries to delimit species. However, it needs to be applied as part of an integrative taxonomic approach that simultaneously assesses cytogenetic, molecular, physiological and morphological traits in order to reach more robust species delimitations.