A new substance to relax polychaete worms (Annelida) prior to morphological study

By

Bonyadi Naeini, Alieh
Rahimian, Hassan
Glasby, Christopher John

Type

Article

Date of Publication

2016

Original Publication

ZooKeys

Volume

594

Pages

1-9

Subjects

Carvacrol , narcotize , Polychaeta , specimen preparation

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.594.8061

 

Abstract

A variety of chemical substances have been used to relax and/or immobilize polychaete worms, and other invertebrates, prior to specimen preparation for morphological examination. To solve difficulties encountered during the study of nereidid polychaetes (Annelida: Phyllodocida), an experiment was designed and carried out to investigate a new relaxing agent to immobilize nereidid specimens and stimulate pharynx eversion. The new substance, Dentol® (Khoraman laboratory, Iran), a dental anesthetic and antiseptic medicine containing 10% Carvacrol as the effective ingredient, was used for the first time and compared with other substances that have been used traditionally in polychaete studies. Crosstab analysis showed significant differences between different treatment groups, with Dentol® providing much better results for all considered criteria.