PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera

By

Wahlberg, Niklas
Peña, Carlos
Ahola, Milla
Wheat, Christopher
Rota, Jadranka

Type

Article

Date of Publication

2016

Original Publication

ZooKeys

Volume

596

Pages

129-141

Subjects

Lepidoptera , molecular systematics , phylogenetics , phylogenomics

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.596.8399

 

Abstract

We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.