The complete mitochondrial genome of the Chinese Daphnia pulex (Cladocera, Daphniidae)

By

Geng, Xuexia
Cheng, Ruixue
Xiang, Tianyi
Deng, Bin
Wang, Yaling
Deng, Daogui
Zhang, Haijun

Type

Article

Date of Publication

2016

Original Publication

ZooKeys

Volume

615

Pages

47-60

Subjects

Daphnia pulex , gene order , mitochondrial genome , secondary structure

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.615.8581

 

Abstract

Daphnia pulex has played an important role in fresh-water ecosystems. In this study, the complete mitochondrial genome of Daphnia pulex from Chaohu, China was sequenced for the first time. It was accomplished using long-PCR methods and a primer-walking sequencing strategy with genus-specific primers. The mitogenome was found to be 15,306 bp in length. It contained 13 protein-coding genes, two rRNA genes, 22 tRNA genes and a typical control region. This research revealed an overall A+T content of 64.50%. All of the 22 typical animal tRNA genes had a classical clover-leaf structure except for trnS1, in which its DHU arm simply formed a loop. The lengths of small and large rRNA were 744 bp and 1,313 bp, respectively. The A+T-rich region was 723 bp in length, which is longer than that from the North American species (689 bp). In terms of structure and composition, many similarities were found between the Chinese and North American Daphnia pulex.