Molecular and morphological identification of Biomphalaria species from the state of São Paulo, Brazil

By

Palasio, Raquel
Guimarães, Marisa
Ohlweiler, Fernanda
Tuan, Roseli

Type

Article

Date of Publication

2017

Original Publication

ZooKeys

Volume

668

Pages

Nov-32

Subjects

Biomphalaria , COI , DNA barcoding , morphological taxonomy , schistosomiasis , species identification

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.668.10562

 

Abstract

DNA barcoding and morphological characters were used to identify adult snails belonging to the genus Biomphalaria from 17 municipalities in the state of São Paulo, Brazil. The DNA barcode analysis also included twenty-nine sequences retrieved from GenBank. The final data set of 104 sequences of the mitochondrial cytochrome oxidase I (COI) gene was analyzed for K2P intraspecific and interspecific divergences, through tree-reconstruction methods (Neighbor-Joining, Maximum Likelihood and Bayesian inference), and by applying different models (ABGD, bPTP, GMYC) to partition the sequences according to the pattern of genetic variation. Twenty-seven morphological parameters of internal organs were used to identify specimens. The molecular taxonomy of Biomphalaria agreed with the morphological identification of specimens from the same collection locality. DNA barcoding may therefore be a useful supporting tool for identifying Biomphalaria snails in areas at risk for schistosomiasis.