Morphological characterization of immature stages of Habrobracon hebetor (Hymenoptera, Braconidae) ectoparasitoid of Ephestia kuehniella (Lepidoptera, Pyralidae)

By

Pezzini, Cleder
Mundstock Jahnke, Simone
Köhler, Andreas

Type

Article

Date of Publication

2017

Original Publication

Journal of Hymenoptera research

Volume

60

Pages

157-171

Subjects

bionomy , Electro micrographs , parasitoid , Pre-imaginal period

Contributed by

Pensoft Publishers

Language

English

Published by

Washington, D.C International Society of Hymenopterists

Identifiers

DOI: https://doi.org/10.3897/jhr.60.20104

 

Abstract

Habrobracon hebetor (Say) is a cosmopolitan idiobiont braconid which parasitizes Pyralidae larvae, a pest of stored products, such as Ephestia kuehniella (Zeller). The objective was to describe the morphology of immature forms of H. hebetor and morphological changes throughout its development. Mated females of H. hebetor were individualized in Petri dishes containing larvae of E. kuehniella for parasitism for six hours. Then, the females were removed, leaving only the eggs placed on the host. The development was evaluated every 12 hours, recording all stages and changes until the emergence of adults. Using stereoscopic optical and scanning electron microscopy, photographs of immature individuals were taken. The results showed that this parasitoid completes its development between 10–12 days. There were stages overlaps during egg to adult development. Eggs are hymenopteriform, with a smooth surface. According to cephalic capsule and larval length measurements of H. hebetor, it was possible to determine four instars. In general, the instars are similar to each other, differing mainly in the size and shape of segments. Larvae present a gradual loss of transparency, becoming opaquer at each successive instar. Last instar larvae distanced from the host to form the cocoon and to pupate. This study was relevant for a better understanding of the physiological interactions between E. kuehniella and H. hebetor.