Monocillium gamsii sp. nov. and Monocillium bulbillosum: two nematode-associated fungi parasitising the eggs of Heterodera filipjevi

By

Ashrafi, Samad
Stadler, Marc
Dababat, Abdelfattah A.
Richert-Pöggeler, Katja R.
Finckh, Maria R.
Maier, Wolfgang

Type

Article

Date of Publication

2017

Original Publication

MycoKeys

Volume

27

Pages

21-38

Subjects

Egg-parasitic fungi , ITS , LSU , molecular phylogeny , new species , Niessliaceae , plant parasitic nematodes , RPB1 , taxonomy , tef

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers 2011-

Identifiers

DOI: https://doi.org/10.3897/mycokeys.27.21254

 

Abstract

Monocillium gamsii sp. nov. (Ascomycota, Hypocreales, Niessliaceae) isolated from eggs of the cereal cyst nematode Heterodera filipjevi is described and illustrated based on morphological and molecular phylogenetic evidence. The new taxon discovered in wheat fields in Turkey destructively parasitises nematode eggs. The infected eggs were readily colonised by the fungus, which produced microsclerotia. The fungus could be grown on artificial media and the parasitism of M. gamsii towards H. filipjevi was reproducible in vitro. Hyphae penetrating the nematode eggs entirely colonised the embryo, developed into multicellular chlamydospore and dictyochlamydospore-like structures eventually forming microsclerotia. Molecular and morphological differences and similarities between M. gamsii and its phylogenetically related species are discussed. Monocillium bulbillosum was found to be closely related to the new species. The pathogenicity of M. bulbillosum against H. filipjevi was also assayed in vitro because of its sister group relationship to M. gamsii revealing that this species was also capable of colonising eggs of H. filipjevi.