Multidirectional chromosome painting in Synallaxis frontalis (Passeriformes, Furnariidae) reveals high chromosomal reorganization, involving fissions and inversions

By

Kretschmer, Rafael
Lima, Vanusa Lilian Camargo de
de Souza, Marcelo Santos
Costa, Alice Lemos
O'Brien, Patricia C. M.
Ferguson-Smith, Malcolm
Herculano Corrêa de Oliveira, Edivaldo
Gunski, Ricardo José
Garnero, Analía del Valle

Type

Article

Date of Publication

2018

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

12

Series / Issue

Issue: 1

Pages

97-110

Subjects

Avian cytogenetics , chromosome fission , chromosome painting , intrachromosomal rearrangements , macrochromosome syntenies

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/CompCytogen.v12i1.22344

 

Abstract

In this work we performed comparative chromosome painting using probes fromGallusgallus(GGA) Linnaeus, 1758 andLeucopternisalbicollis(LAL) Latham, 1790 inSynallaxisfrontalisPelzeln, 1859 (Passeriformes, Furnariidae), an exclusively Neotropical species, in order to analyze whether the complex pattern of intrachromosomal rearrangements (paracentric and pericentric inversions) proposed for Oscines and Suboscines is shared with more basal species.S.frontalishas 82 chromosomes, similar to most Avian species, with a large number of microchromosomes and a few pairs of macrochromosomes. We found polymorphisms in pairs 1 and 3, where homologues were submetacentric and acrocentric. Hybridization of GGA probes showed syntenies in the majority of ancestral macrochromosomes, except for GGA1 and GGA2, which hybridized to more than one pair of chromosomes each. LAL probes confirmed the occurrence of intrachromosomal rearrangements in the chromosomes corresponding to GGA1q, as previously proposed for species from the order Passeriformes. In addition, LAL probes suggest that pericentric inversions or centromere repositioning were responsible for variations in the morphology of the heteromorphic pairs 1 and 3. Altogether, the analysis of our data on chromosome painting and the data published in other Passeriformes highlights chromosomal changes that have occurred during the evolution of Passeriformes.