A DNA barcode library for ground beetles of Germany: the genus Amara Bonelli, 1810 (Insecta, Coleoptera, Carabidae)

By

Raupach, Michael
Hannig, Karsten
Moriniere, Jerome
Hendrich, Lars

Type

Article

Date of Publication

2018

Original Publication

ZooKeys

Volume

759

Pages

57-80

Subjects

Central Europe , cytochrome c oxidase subunit I , German Barcode of Life , mitochondrial DNA , molecular specimen identification , Zabrus

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.759.24129

 

Abstract

The genusAmaraBonelli, 1810 is a very speciose and taxonomically difficult genus of the Carabidae. The identification of many of the species is accomplished with considerable difficulty, in particular for females and immature stages. In this study the effectiveness of DNA barcoding, the most popular method for molecular species identification, was examined to discriminate various species of this genus from Central Europe. DNA barcodes from 690 individuals and 47 species were analysed, including sequences from previous studies and more than 350 newly generated DNA barcodes. Our analysis revealed unique BINs for 38 species (81%). Interspecific K2P distances below 2.2% were found for three species pairs and one species trio, including haplotype sharing betweenAmaraalpina/AmaratorridaandAmaracommunis/Amaraconvexior/Amaramakolskii. This study represents another step in generating an extensive reference library of DNA barcodes for carabids, highly valuable bioindicators for characterizing disturbances in various habitats.