Corrigendum for: “Oomycete-specific ITS primers for identification and metabarcoding” published in MycoKeys, doi: 10.3897/mycokeys.14.9244

By

Riit, Taavi
Tedersoo, Leho
Drenkhan, Rein
Runno-Paurson, Eve
Kokko, Harri
Anslan, Sten

Type

Article

Date of Publication

2018

Original Publication

NeoBiota : advancing research on alien species and biological invasions

Volume

41

Pages

119-120

Subjects

community analysis , community barcoding , corrigendum , ITS , next generation sequencing , oomycete , soil community

Contributed by

Pensoft Publishers

Language

English

Published by

Sophia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/mycokeys.41.30558

 

Abstract

The oomycete-specific ITS primers published by Riit et al. (2016) have been put to use in the scientific community working with oomycetes.  Recently, however, it has been brought to our attention that the sequences of the primers ITS1oo and ITS3oo shown in the first Figure of the published manuscript are incomplete, when compared to the sequences of the same primers as listed on the UNITE website. This discrepancy is derived from re-checking primer sequences from tube labels that are restricted to the first 18 bases.Closer examination revealed that the sequence of primer ITS1oo in Figure 1 is missing one nucleotide from the 3’ end and the primer ITS3oo is missing two nucleotides from the 3’ end. These errors are expected to reduce relative primer specificity to Oomycetes, which probably results in lower proportion of this group in metabarcoding studies. We hereby provide the updated figure (Figure 1) with correct information. We apologize to all users of these erroneous primers for their suboptimal performance. We are grateful to Dr. Diana Marčiulynienė and Dr. Sannakajsa Velmala for pointing out these problems.