New insights into the karyotype evolution of the genus Gampsocleis (Orthoptera, Tettigoniinae, Gampsocleidini)

By

Kociński, Maciej
Grzywacz, Beata
Chobanov, Dragan
Warchałowska-Śliwa, Elżbieta

Type

Article

Date of Publication

2018

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

12

Series / Issue

Issue: 4

Pages

529-538

Subjects

18S rDNA , Ag-NOR , chromosome evolution , Fish , fluorochrome staining , Gampsocleis , Orthoptera , telomeric repeats

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/CompCytogen.v12i4.29574

 

Abstract

Five species belonging to the genusGampsocleisFieber, 1852 were analyzed using fluorescencein situhybridization (FISH) with 18S rDNA and telomeric probes, as well as C-banding, DAPI/CMA3staining and silver impregnation. The studied species showed two distinct karyotypes, with 2n = 31 (male) and 2n = 23 (male) chromosomes. The drastic reduction in chromosome number observed in the latter case suggests multiple translocations and fusions as the main responsible that occurred during chromosome evolution. Two groups of rDNA distribution were found inGampsocleisrepresentatives analyzed. Group 1, with a single large rDNA cluster on the medium-sized autosome found in four species, carried in the haploid karyotype. Group 2, represented only byG.abbreviata, was characterized by the presence of two rDNA signals. TTAGG telomeric repeats were found at the ends of chromosome arms as expected. The rDNA clusters coincided with active NORs and GC-rich segments.