Species identification of European forest pathogens of the genus Milesina (Pucciniales) using urediniospore morphology and molecular barcoding including M. woodwardiana sp. nov.

By

Bubner, Ben
Buchheit, Ramona
Friedrich, Frank
Kummer, Volker
Scholler, Markus

Type

Article

Date of Publication

2019

Original Publication

NeoBiota : advancing research on alien species and biological invasions

Volume

48

Pages

Jan-40

Subjects

Abies alba , Europe , GBOL , germ pores , host alternation , Polypodiales , Uredinopsis

Contributed by

Pensoft Publishers

Language

English

Published by

Sophia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/mycokeys.48.30350

 

Abstract

Species of rust fungi of the genusMilesina(Pucciniastraceae, Pucciniales) are distributed mainly in northern temperate regions. They host-alternate between needles of fir (Abiesspp.) and fronds of ferns (species of Polypodiales).Milesinaspecies are distinguished based on host taxonomy and urediniospore morphology. In this study, 12 species ofMilesinafrom Europe were revised. Specimens were examined by light and scanning electron microscopy for urediniospore morphology with a focus on visualising germ pores (number, size and position) and echinulation. In addition, barcode loci (ITS, nad6, 28S) were used for species delimitation and for molecular phylogenetic analyses. Barcodes of 72Milesinaspecimens were provided, including 11 of the 12 species.Whereas urediniospore morphology features were sufficient to distinguish all 12Milesinaspecies except for 2 (M.blechniandM.kriegeriana), ITS sequences separated only 4 of 11 species. Sequencing with 28S and nad6 did not improve species resolution. Phylogenetic analysis, however, revealed four phylogenetic groups withinMilesinathat also correlate with specific urediniospore characters (germ pore number and position and echinulation). These groups are proposed as new sections withinMilesina(sectionsMilesina,VogesiacaeM. Scholler & Bubner,sect. nov.,ScolopendriorumM. Scholler & Bubner,sect. nov.andCarpaticaeM. Scholler & Bubner,sect. nov.). In addition,MilesinawoodwardianaBuchheit & M. Scholler,sp. nov.onWoodwardiaradicans, a member of the type section Milesina, is newly described. An identification key for EuropeanMilesinaspecies, based on urediniospore features, is provided.