Recent study of the water scavenger beetle subfamily Acidocerinae in the Neotropical region has uncovered numerous undescribed species that are not able to be placed in existing genera. Here, we describe three new genera to accommodate 17 of these new species from South America:Aulonocharesgen. nov.forAulonochareslingulatussp. nov.(French Guiana, Suriname),Aulonocharesnovoairensissp. nov.(Brazil), andAulonocharestubulussp. nov.(Brazil, Guyana, Suriname, Venezuela);Ephydrolithusgen. nov.forEphydrolithushamadaesp. nov.(Brazil),Ephydrolithusminorsp. nov.(Brazil),Ephydrolithusogmossp. nov.(Brazil),Ephydrolithusspiculatussp. nov.(Brazil), andEphydrolithustelisp. nov.(Brazil); andPrimocerusgen. nov.forPrimoceruscuspidissp. nov.(Venezuela),Primocerusgigassp. nov.(Venezuela),Primocerusneutrumsp. nov.(Guyana, Suriname, Venezuela),Primocerusocellatussp. nov.(Venezuela),Primoceruspetilussp. nov.(Brazil),Primoceruspijiguaensesp. nov.(Venezuela),Primocerusmaipuresp. nov.(Venezuela),Primocerussemipubescenssp. nov.(Guyana), andPrimocerusstriatolatussp. nov.(Suriname). The genusEphydrolithusgen. nov.is currently known to be restricted to seepages in the mountainous regions of the Brazilian Shield.Aulonocharesgen. nov.andPrimocerusgen. nov.are both currently only known from the Guiana Shield, though widespread in that region where they are associated with streams and seeps. We present differential diagnoses, maps, habitat details, and illustrations of all new genera and species here described.