A molecular study of Neophyllaphis varicolor (Hemiptera, Aphididae) in Costa Rica

By

Zuñiga-Centeno, Adonay
Sandoval-Carvajal, Izayana
Montero-Astúa, Mauricio
Villalobos Muller, William
Nguyễn, Bảo Quốc
Pérez Hidalgo, Nicolás

Type

Article

Date of Publication

2019

Original Publication

ZooKeys

Volume

865

Pages

123-135

Subjects

Aphids , cytochrome c oxidase subunit I , DNA barcoding , elongation factor I , integrative taxonomy , phylogenetic analysis , Podocarpus

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.865.35213

 

Abstract

The genusNeophyllaphis(Takahashi) (Aphididae: Neophyllaphidinae) is composed of 18 species; however, in the Americas only nine species have been reported previously. A new species,NeophyllaphisvaricolorMiller & Halbert, was described in 2014 in USA. Colonies resembling those of this new species have been observed in Costa Rica onPodocarpusspp. In order to determine ifN.varicoloris also present in Costa Rica, we sampledNeophyllaphiscolonies fromPodocarpusfalcatusandP.chinensis. Additionally, we sampled individuals fromPodocarpussp. in Spain and Vietnam. DNA of each sample was extracted and used to amplify and sequence the cytochromecoxidase subunit I (COI) and elongation factor I (EF-1α) partial regions. According to morphological characteristics, sequences comparisons done in GenBank and BOLD, and phylogenetic analyses, the colonies collected fromPodocarpusspp. in Costa Rica and the colony from Vietnam corresponded to the speciesN.varicolor. To the best of our knowledge this is the first report of the presence ofN.varicolorin Central America and Vietnam.