Development of new cytogenetic markers for Thinopyrum ponticum (Podp.) Z.-W. Liu & R.-C. Wang

By

Kroupin, Pavel Yu.
Kuznetsova, Victoria M
Nikitina, Ekaterina
Martirosyan, Yury
Karlov, Gennady
Divashuk, Mikhail

Type

Article

Date of Publication

2019

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

13

Series / Issue

Issue: 3

Pages

231-243

Subjects

copy number , Cytogenetic Markers , fluorescence in situ hybridization , quantitative PCR , repetitive DNA , Thinopyrum ponticum

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/CompCytogen.v13i3.36112

 

Abstract

Thinopyrum ponticum(Podpěra, 1902) Z.-W. Liu & R.-C.Wang, 1993 is an important polyploid wild perennial Triticeae species that is widely used as a source of valuable genes for wheat but its genomic constitution has long been debated. For its chromosome identification, only a limited set of FISH probes has been used. The development of new cytogenetic markers forTh. ponticumchromosomes is of great importance both for cytogenetic characterization of wheat-wheatgrass hybrids and for fundamental comparative studies of phylogenetic relationships between species. Here, we report on the development of five cytogenetic markers forTh. ponticumbased on repetitive satellite DNA of which sequences were selected from the whole genome sequence ofAegilops tauschiiCosson, 1849. Using real-time quantitative PCR we estimated the abundance of the found repeats: P720 and P427 had the highest abundance and P132, P332 and P170 had lower quantity inTh. ponticumgenome. Using fluorescencein situhybridization (FISH) we localized five repeats to different regions of the chromosomes ofTh. ponticum. Using reprobing multicolor FISH we colocalized the probes between each other. The distribution of these found repeats in the Triticeae genomes and its usability as cytogenetic markers for chromosomes ofTh. ponticumare discussed.