Phylogeny and species delimitations in the entomopathogenic genus Beauveria (Hypocreales, Ascomycota), including the description of B. peruviensis sp. nov.

By

Bustamante, Danilo
Oliva, Manuel
Leiva, Santos
Mendoza, Jani E.
Bobadilla, Leidy
Angulo, Geysen
Calderon, Martha

Type

Article

Date of Publication

2019

Original Publication

MycoKeys

Volume

58

Pages

47--68

Subjects

Beauveria , Fungal diversity , multi-locus phylogeny , Peru , polyphasic approaches , species delimitation

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers 2011-

Identifiers

DOI: https://doi.org/10.3897/mycokeys.58.35764

 

Abstract

The genusBeauveriais considered a cosmopolitan anamorphic and teleomorphic genus of soilborne necrotrophic arthropod-pathogenic fungi that includes ecologically and economically important species. Species identification inBeauveriais difficult because of its structural simplicity and the lack of distinctive phenotypic variation. Therefore, the use of multi-locus sequence data is essential to establish robust species boundaries in addition to DNA-based species delimitation methods using genetic distance, coalescent, and genealogical concordance approaches (polyphasic approaches). In this regard, our study used multilocus phylogeny and five DNA-based methods to delimit species inBeauveriausing three molecular makers. These polyphasic analyses allowed for the delimitation of 20–28 species inBeauveria, confirming cryptic diversity in five species (i.e.B. amorpha,B. bassiana,B. diapheromeriphila, andB. pseudobassiana) and supporting the description ofB. peruviensisas a new taxon from northeastern Peru. The other five species were not evaluated as they did not have enough data (i.e.B. araneola,B. gryllotalpidicola,B. loeiensis,B. medogensis, andB. rudraprayagi). Our results demonstrate that the congruence among different methods in a polyphasic approach (e.g. genetic distance and coalescence methods) is more likely to show reliably supported species boundaries. Among the methods applied in this study, genetic distance, coalescent approaches, and multilocus phylogeny are crucial when establishing species boundaries inBeauveria.