Studies on the bivalve family Sphaeriidae in North Africa are very limited at the surface water level, but even more for caves. During an expedition in 2019 to the Ait M’hamed cave (Oum Er Rabia Basin), six specimens of the genus Pisidium were collected. Morphometric and genetic analyses showed that these individuals belong to the species Pisidium subtruncatum Malm, 1855. This work is the first step towards future exploration of cave Sphaeriidae in North Africa.